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Journal of Education, Health and Sport

Effect of an antidisbiotic agent on the biosynthesis of fatty acids of liver lipids of rats which received palm oil on the background of dysbiosis
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  • Effect of an antidisbiotic agent on the biosynthesis of fatty acids of liver lipids of rats which received palm oil on the background of dysbiosis
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  3. Vol. 12 No. 5 (2022) /
  4. Research Articles

Effect of an antidisbiotic agent on the biosynthesis of fatty acids of liver lipids of rats which received palm oil on the background of dysbiosis

Authors

  • A. Levitsky Odessa National Technology University
  • A. Markov Lviv National Medical University named after Danylo Galytskij
  • V. Velichko Odessa National Medical University
  • I. Selivanskaya Odessa National Medical University
  • A. Lapinskaya Odessa National Technology University

DOI:

https://doi.org/10.12775/JEHS.2022.12.05.023

Keywords

palm oil, fatty acids, liver lipids, dysbiosis, antidysbiotic agent

Abstract

Background. To determine the effect of antidysbiotic agent on the biosynthesis of fatty acids of lipids in the liver of rats fed a high-fat diet (HFD) with palm oil against the background of dysbiosis.

Methods. The HFD contained 15% palm oil. In a biological experiment, white rats were used, divided into 4 groups: the 1st group received a fat-free diet (FFD), the 2nd, 3rd, and 4th received HFD. In rats of the 3rd and 4th groups, dysbiosis was reproduced using lincomycin. Rats of the 4th group from the first day of the experiment received an antidysbiotic agent (inulin + quercetin, ADA) with food. The duration of feeding is 39 days. Liver lipids were divided into 3 fractions: neutral lipids (NL), phospholipids (PL), and free fatty acids (FFA). The fatty acid composition of the fractions was determined by gas chromatography. The “activities” of fatty acid synthase, palmitic acid elongase, and stearyl-CoA- desaturase (SCD18 and SCD16) were determined.

Results. The presence of all classes of fatty acids (FA) in the liver lipids of rats treated with FFD was established.  Consumption of HFD with palm oil increased the content of NL in the liver by 6 times (gr. 2), and in group 3 by 8 times.  The introduction of ADA reduces the content of NL almost to the level of the 1st group.  The content of ω-3 polyunsaturated fatty acids (PUFA) decreases in the PL fraction in rats of the 3rd group and is restored in rats of the 4th group. In rats treated with HFD, the "activity" of synthase, SCD18 and very strongly SCD16 are reduced.

Conclusion: The negative effect of palm oil on the background of dysbiosis on the biosynthesis of fatty acids in the liver, leading to hepatic steatosis and deficiency of ω-3 PUFA, can be prevented by the use of an antidysbiotic agent.

References

Levitsky AP, Markov AV, Pupin TI, Zubachik VM. Normalization of the metabolism of essential fatty acids of phosolipids of the liver of rats received with palm oil on the background of disbiosis by the phytopreparation "Kvertulin". Phytotherapy. Magazine. 2021;1:35-39. (In Ukrainian)

Levitsky A. P., Gozhenko A. I., Velichko V. V., Selivanskaya I. A. The effect of dietary fat supplements on the activity of palmitic and stearic acid desaturases based on the results of a study of the fatty acid composition of neutral lipids in blood serum and liver of rats receiving a fat-free diet. Journal of Education, Health and Sport. 2022;12(1):197-206. eISSN 2391-8306

Levitsky AP, Makarenko OA, Levchenko OM [and others]. Bioflavonoid hepatoprotectors. Odessa: KP OGT, 2014: 86. (in Russian)

Levitsky AP, Makarenko OA, Demyanenko SA. Methods of experimental dentistry (teaching aid). Simferopol, Tarpan, 2018:78. (in Russian)

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Khodakov IV, Tkachuk VV, Velichko VI [and others]. The fatty acids composition of liver lipids of rats which received the palm oil and lincomycin. Marine Medicine Bulletin. 2017;1(74):145-152. (in Russian)

Levitsky AP, Makarenko OA, Khodakov IV. Methods to investigate fats and oils. Odessa: KP OGT, 2015:32. (in Russian)

Svendsen K, Olsen T, Nordstrand Rusvik TC, Ulven SM, Holven KB, Retterstøl K, Telle-Hansen VH. Fatty acid profile and estimated desaturase activities in whole blood are associated with metabolic health. Lipids in Health and Disease 2020;19:102. DOI: 10.1186/s12944-020-01282-y

Rybin VG, Blinov IuG. Antimicrobial properties of lipids. News of the Pacific Research Fisheries Center. 2001;129:179-196. (in Russian)

Shipko ES, Duvanova OV. Changing the spectrum of fatty acids as one of the mechanisms of adaptation/persistence of microorganisms. Journal of Microbiol. 2019;5:109-118. (in Russian)

Ruker KM, Daman D, Khansen DM [and others]. The method of obtaining lipids containing polyunsaturated fatty acids (variants and methods of cultivation of microorganisms culturing these lipids). Patent RU 2326171 С2. Published on 10.06.2008. Bulletin no. 16.

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Published

2022-05-31

How to Cite

1.
LEVITSKY, A., MARKOV, A., VELICHKO, V., SELIVANSKAYA, I. & LAPINSKAYA, A. Effect of an antidisbiotic agent on the biosynthesis of fatty acids of liver lipids of rats which received palm oil on the background of dysbiosis. Journal of Education, Health and Sport [online]. 31 May 2022, T. 12, nr 5, s. 292–303. [accessed 29.3.2023]. DOI 10.12775/JEHS.2022.12.05.023.
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Issue

Vol. 12 No. 5 (2022)

Section

Research Articles

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Copyright (c) 2022 A. Levitsky, A. Markov, V. Velichko, I. Selivanskaya, A. Lapinskaya

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0

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