Journal of Education, Health and Sport

Aim. Compare the effect of different pathogens on the development of kidney dysbiosis.

Methods. The pathogens used were hydrazine sulfate, prednisolone, cyclophosphamide, lincomycin, and lipopolysaccharide (LPS). The condition of kidney dysbiosis was assessed in rats by a decrease in lysozyme activity and an increase in urease activity.

Results. A decrease in the activity of lysozyme in the kidneys under the action of pathogens was established, which, when calculated per 1 mg of a pathogen, is hundreds of times stronger in LPS. All pathogens cause an increase in urease activity in the kidneys, which, when calculated per 1 mg of a pathogen, is hundreds of times stronger in LPS. A similar effect is exerted on the degree of dysbiosis, determined by the ratio of the relative activities of urease and lysozyme.

Conclusion. All pathogens (except lincomycin) cause the development of dysbiosis in the kidneys, due to the possible bacteriolysis of tissue gram-negative bacteria with the release of LPS.

Levitsky AP. Disbiotic syndrome: etiology, pathogenesis, clinic, prevention and treatment. Dentistry Bulletin. 2019; 10 (special issue): 14-20. (in Russian)

Dyatlov IA. Actual problems of medical microbiology. ZhMEI. 2013; 1: 88-93. (in Russian)

Shulpekova Y.O. The gut microbiome as a special organ.

RZhGGK. 2014; 6: 82-88. (in Russian)

Levitsky AP, Makarenko OA, Denga OV [and others]. The experimental methods of restoration and estimation of the degree of dysbiosis in oral tissues. Dentistry Bulletin. 2010; 2: 22-23. (in Russian)

Levashov PA, Matolygina DA, Ovchinnikova ED [and others]. New sorbent based on covalently immobilized lysozyme to remove bacterial lipopolysaccharide (endotoxin) from biological fluids. Biochemistry. 2019; 84(1): 100-108. (in Russian)

Pustovoyt IP, Levitsky AP, Knava OE. Lysozyme and elastase activity in kidney tissue of immunodeficient rats under the action of lipopolysaccharide. Bulletin of Marine Medicine. 2013; 3(60): 65-67. (in Russian)

Levitsky AP, Ostafiichuk MA, Uspenskii OE [and others]. The influence of different pathogens on the lysozyme activity into tissues of rat oral cavity. Journal of Education, Health and Sport. 2017; 7(8): 1070-1081.

Levitsky AP, Gozhenko AI, Stepan VT [and others]. Dysbiotic aspects of pathogenesis and antidysbiotic prophylactics of experimental nephropathy. Journal of Education, Health and Sport. 2017; 7(6): 1124-1136.

Levitsky AP. Lysozyme instead of antibiotics. Оdеssа, KP OGT, 2005: 74. (in Russian)

Gavrikova LM, Segen IT. Urease activity of oral liquid in patients with acute odontogenic infection of maxillo-facial part. Stomatology. 1996; The extra issue: 49-50. (in Russian)

Truhacheva NV. Mathematical Statistics in biomedical research using application package Statistica. Moskow, GJeOTAR-Media, 2012: 379. (in Russian)

Levitsky AP, Makarenko OA, Selivanskaya IA [and others]. Enzymatic methods for determination of oral dysbiosis for screening pro- and prebiotics: method guidelines. Кiev, GFC, 2007: 22. (in Russian)

Levitsky AP, Demyanenko SA, Tsiselskiy YuV. The antimicrobic function of liver. Оdеssа, KP OGT, 2011:141. (in Russian)