The study of the prooxidant-antioxidant equilibrium in the kidney tissue and blood on the background of ceruloplasmin administration under the conditions of acute kidney injury

I. Zamorskii, T. Unhuryan, S. Batranovska



Acute kidney injury (AKI) remains a relevant medical problem. The promising direction of the AKI treatment is the use of the antioxidants that inhibit free radical processes and prevent the destruction of cell membranes within pathological processes. Ceruloplasmin (CP) is the main antioxidant of blood plasma, that demonstrates membrane protecting effects and antitoxic activity.

The aim of the study was to establish the influence of a single-dose administration of ceruloplasmin on the processes of the peroxidation in the kidney tissue and blood under the conditions of acute kidney injury.

Materials and methods. The influence of ceruloplasmin on the prooxidant-antioxidant equilibrium in the kidney tissue and blood was studied in an experiment on white non-line rats under the conditions of rhabdomyolysis-induced acute kidney injury. The acute kidney injury was modeled by intramuscular administration of 50% glycerol solution at a dose of 8 mg/kg. Ceruloplasmin was administered intraperitoneally at a dose of 7 mg/kg/day. The level of main indicators of prooxidant-antioxidant balance in blood and kidney tissue was determined in 24 h after glycerol administration for induction of acute kidney injury.

Research results and their discussion. There was a deep disruption of the prooxidant-antioxidant equilibrium as a result of the acute kidney injury. AKI causes an activation of the prooxidant processes by increasing the level of the malonic dialdehyde and the oxidized proteins in the kidney tissue and blood. AKI also reduces the antioxidant protection by decreasing the activity of the antioxidant enzymes in the kidneys and blood plasma.

A single-dose administration of ceruloplasmin improved antioxidant protection and reduced peroxidation of lipids and proteins. There was a decrease in levels of malondialdehyde and oxidative modification of proteins. At the same time, the antioxidant protection intensified due to an increase in the activity of glutathione peroxidase and catalase in the kidneys and blood plasma.

Conclusions. A single administration of ceruloplasmin influences prooxidant-antioxidant balance, reducing the content of lipid and protein peroxidation products and increasing the activity of antioxidant enzymes.


acute kidney injury; oxidative stress; prooxidant-antioxidant balance; antioxidants; ceruloplasmin

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Birben E, Sahiner U.M., Sackesen C., Erzurum S., Kalayci O. (2012) Oxidative stress and antioxidant defense. WAO Journal, 5, 10-19.

Dennis J. M., Witting P. K. (2017) Protective Role for Antioxidants in Acute Kidney Disease. Nutrients., 9, 718. P.25.

Elshama S., Abdalla M.E., Mohamed A.M. (2018) Role of Natural Antioxidants in Treatment of Toxicity. Journal of Toxicological Analysis, 1, 3

Hasanuzzaman M., Bhuyan M.H.M.B., Zulfiqar F., Raza A., Mohsin S.M., Mahmud J.A., Fujita M., Fotopoulos V. (2020) Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants, 9, 681; doi:10.3390/antiox9080681

Golenkina E.A., Viryasova G.M., Galkina S.I., Gaponova T.V., Sud’ina G.F., Sokolov A.V. (2018) Fine Regulation of Neutrophil Oxidative Status and Apoptosis by Ceruloplasmin and Its Derivatives. Cells. 7, 8. doi:10.3390/cells7010008

Linder M.C. (2016) Ceruloplasmin and Other Copper Binding Components of Blood Plasma and Their Functions: An Update. Metallomics, 1-37. doi: 10.1039/C6MT00103C.

Makris K., Spanou L. (2016) Acute Kidney Injury: Definition, Pathophysiology and Clinical Phenotypes. The Clinical Biochemist, 37 (2), 85-98.

Palipoch S. (2013) A review of oxidative stress in acute kidney injury: protective role of medicinal plants-derived antioxidants. Afr. J. Tradit Complement Altern Med., 10 (4), 88-93.

Ramos D., Mar D., Ishida M., Vargas R., Gaite M., Montgomery A. et al. (2016) Mechanism of Copper Uptake from Blood Plasma Ceruloplasmin by Mammalian Cells. PLoS ONE, 11(3), e0149516. doi: 10.1371/journal.pone.0149516

Samygina V.R., Sokolov A.V., Bourenkov G., Petoukhov M.V., Pulina M.O., Zakharova E.T, et al. (2013) Ceruloplasmin: Macromolecular Assemblies with Iron Containing Acute Phase Proteins. Plos one, 7(8), 1-12.

Tamay-Cacha F., Quintana-Pereza J. C., Trujillo-Ferraraa J. G., Cuevas-Hernandeza R.I., Del Valle-Mondragonb L., Garcıa-Trejoc E. M., Arellano-Mendoza M.G. (2016) A review of the impact of oxidative stress and some antioxidant therapies on renal damage. Renal failure, 38 (2), 171–175.

Tsai W.H., Huang S.T., Liu W.C., Chen LW, et al. (2015) High risk of rhabdomyolysis and acute kidney injury after traumatic limb compartment syndrome. Ann Plast Surg. 74, 2, 158-61.

Vashchenko G., MacGillivray R.T.A. (2013) Multi-copper oxidases and human iron metabolism. Nutrients, 5, 2313-25.

Zamorskii I.I., Drachuk V.M., Horoshko O.M. (2016) The nephroprotective effects of taurine in acute kidney injury due to rhabdomyolysis. Biophysics, 6 (61), 1036-38.

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1223 Journal of Education, Health and Sport eISSN 2391-8306 7

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