Cell reactions of inflammation focus in carrageenan secondary chronic inflammation on the background of substance P blockade

O. M. Shevchenko, V. O. Sych

DOI: http://dx.doi.org/10.12775/JEHS.2020.10.11.019


The aim of the study was to determine the features of cellular reactions of the inflammatory focus in carrageenan secondary chronic inflammation against the background of blockade of substance P. Experimental prospective controlled randomized study was performed on 132 WAG rats in series of 6 animals in each series: in control and for 6 hours; 1, 2, 3, 5, 7, 10, 14, 21, 28 days in the conditions of the natural course of carrageenan inflammation and with the use of a pharmacological preparation, aprepitant – an inhibitor of NK-1 receptors. During the observation period of 6 hours to 3 days, neutrophilic, basophilic and eosinophilic leukocytes accumulate (phase of exudation of the inflammatory process). Peaks of neutrophils and basophils occur on day 2 (neutrophils – in the central parts of the inflammatory focus, basophils – in both areas, in the center and on the periphery) with a small predominance of the number of cells in the peripheral parts. The peak number of eosinophils occurs on day 1 and with a slight decrease on day 2 with their predominant location in peripheral areas. From 3–5 days the cellular composition of the infiltrate was characterized by a predominance of lymphocytes, monocytes, macrophages, plasma cells, tissue basophils, cells of the fibroblastic series, which during blockade of substance P in a single amount appear for 2 days, predominate in peripheral areas (phases of proliferation and organization).


secondary chronic inflammation; focus of inflammation; mediators of inflammation

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Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes / X. Liu, Y. Zhu, W. Zheng, T. Qian, H. Wang, X. Hou // Artif Cells Nanomed Biotechnol. — 2019. — Vol. 47, No. 1. — P. 1628–1634.

Suvas S. Role of substance P neuropeptide in inflammation, wound healing, and tissue homeostasis // J. Immunol. — 2017. — Vol. 199, No. 5. — P. 1543–1552.

Substance P activates human eosinophils / M. Raap, U. Rüdrich, S. Ständer [et al.] // Exp. Dermatol. — 2015. — Vol. 24, No. 7. — P. 557–559.

Substance P (SP) induces expression of functional corticotropin-releasing hormone receptor-1 (CRHR-1) in human mast cells / S. Asadi, K. D. Alysandratos, A. Angelidou [et al.] // J. Invest. Dermatol. — 2012. — Vol. 132, No. 2. — P. 324–329.

Interleukin-12 (IL-12) and IL-23 induction of substance P synthesis in murine T cells and macrophages is subject to IL-10 and transforming growth factor β regulation / A. Blum, T. Setiawan, L. Hang [et al.] // Infect. Immun. — 2008. — Vol. 76, No. 8. — P. 3651–3656.

Neuropeptide substance P and the immune response / A. Mashaghi, A. Marmalidou, M. Tehrani [et al.] // Cell Mol. Life Sci. — 2016. — Vol. 73, No. 22. — P. 4249–4263.

An FDA approved neurokinin-1 receptor antagonist is effective in reducing intraabdominal adhesions when administered intraperitoneally, but not orally / R. Lim, J. M. Morrill, S. G. Prushik [et al.] // J. Gastrointest. Surg. — 2008. — Vol. 12. — P. 1754–1761. — doi: 10.1007/s11605-008-0634-4.

8. Substantiation of the model of chronic (secondary chronic) inflammation / N. A. Klimenko, S. V. Tatarko, A. N. Shevchenko, G. I. Gubina-Vakulik // Experimental and clinical medicine. — 2007. — № 2. — P. 24–28. (in Russian)

Belogurova E. A. Inflammation / E. A. Belogurova, I. V. Budnikova. – SPb. : SZGMU Publishing House. II Mechnikov. — 2014. — 24 p. (in Russian)

Rameshwar P. Hematopoietic modulation by the tachykinins / P. Rameshwar, P. Gascón // Acta Haematol. — 1997 — Vol. 98, No. 2. — P. 59–64.

Effects of chronic tobacco smoking on the distribution of tachykinin receptors in rat pial arteries / N. V. Zakharchuk, V. M. Chertok, V. A. Nevzorova, E. Y. Gonchar // Bull Exp Biol Med. — 2017. — Vol. 163, No. 3. — P. 313–316.

Prenatal nicotinic exposure upregulates pulmonary C-fiber НК1Р expression to prolong pulmonary C-fiber-mediated apneic response / L. Zhao, J. Zhuang, N. Zang [et al.] // Toxicol. Appl. Pharmacol. — 2016. — Vol. 290. — P. 107–115.

Impaired IL-1beta-induced neutrophil accumulation in tachykinin NK1 receptor knockout mice / A. Ahluwalia, C. De Felipe, J. O'Brien [et al.] // Br. J. Pharmacol. — 1998. — Vol. 124, No. 6. — P. 1013–1015.

Role of capsaicin-sensitive nerves and tachykinins in mast cell tryptase-induced inflammation of murine knees / É. Borbély, K. Sándor, A. Markovics [et al.] // Inflamm. Res. — 2016. — Vol. 65, No. 9. — P. 725–736.

The neurokinin 1 receptor and its agonists recruited at the DC-T cell synapse are necessary to promote the survival of activated T cells in vivo / A. T. Larregin, T. L. Sumpter, D. M. Rojas [et al.] // J. Immunol. — 2017. — Vol. 198, Suppl. 1. — P. 220.

Weinstock J. V. Substance P and the regulation of inflammation in infections and inflammatory bowel disease // Acta. Physiol. (Oxf). — 2015. — Vol. 213, No. 2. — P. 453–461.

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Journal of Education, Health and Sport formerly Journal of Health Sciences

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1223 Journal of Education, Health and Sport eISSN 2391-8306 7

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