Journal of Education, Health and Sport

We aimed to study the pathomorphological changes in the structure of intervertebral disc in the early stages of the experimental opioid use. This goal was achieved by the use of histological visualization of the intervertebral disc’s structures. Histological specimens were prepared according to the typical methods using such  specimens as hematoxylin, eosin, azan and due to Heidenhain method, McManus PAS-reaction and Steedman alcian blue method.

The results of this study will let form a pathomorphological basis that could be used for comparative characterization of the growth dynamics of pathomorphological changes in the intervertebral disc’s structures in the early stages and compare these changes to pathomorphological signs in the late stages of experimental opioid use. These findings will provide an opportunity to establish the most appropriate time limits when these pathomorphological changes in the structure of the intervertebral disc develop and to observe the dynamics of these changes in the long term experimental opioid use.

Biriuchkov MIu. The specific features of surgery of herniated intervertebral disks. Zh Vopr Neirokhir Im N N Burdenko. 2005 Oct-Dec; 4:22- 4.

Pinchuk SV. Modeling the lumbar spine computer tomography sizes on median-sagittal sections using regression analysis in healthy young men depending on anthropo-somatotypological indicators. Pinchuk SV, Volkov KS, Kryvko YuYa., Shayuk AV. Aktualni pytannya medychnoi nauky ta praktyky. 2015; 82. Р. 136-42.

Pinchuk SV, Shayuk AV. А clinical value and problems of determination of morphometric parameters of lumbar spine in a norm and at pathology. Review of literature. Ukrainskyi medychnyi almanakh. 2013;16.5. Р.101-106.

Gubin AV. The etiology of acute torticollis in children. In: Proceedings of the scientific-practical conference with international participation. Gubin AV, Ulrich EV, Yalfimov AN, Taschilkin AI. Actual problems of children's traumatology and orthope- dics; 2008; Kazan, Russia. Kazan; 2008. Р.170-171.

Dudin MG. On etiopathogenesis of idiopathic scoliosis. Dudin MG, Pinchuk DYu, Bekshaev SS, et.all. Spine Surgery. 2006;(4):18-25.

Dmitriev AE, Tenants VK, Vavilov SB. Computed tomography in the diagnosis of degener- ative lesions of the spine - intervertebral disc herniation. Clinical Medicine. 2009. 4. P. 93-95.

Kostyuk GY, Vlasyuk VL Magnetic resonance imaging in the diagnosis and treatment of herniated discs. Clinical anatomy and operative surgery. 2008;7. 3. P. 53-56.

Hosten N. Computed tomography of the head and spine. 2nd ed. Hosten N, Liebig T. MED press-inform; 2013. 576 p.

Onysko RM, Paltov EV, Fik VB, Vilkhova IV, Kryvko Yu.Ya. ., Yakymiv N.Ya., Fitkalo OS. Pat. №76564 U Ukraine, IPC A 61 K 31/00 Method of modeling physical opioid dependence in rats. Рatent owner: Danylo Halytskyi Lviv National Medical University. 201u201207124; declared 12.06.2012; publ. 10.01.2013, Bull. №1.

Romeis B. Microscopic technique. M. Medicine. 1953: 71 - 72.

Goralsky LP, Khomich VT, Kononsky OI Edited by Goralsky LP Fundamentals of histological technique and morphofunctional research methods in normal and pathology. Tutorial. Kind. III, corrected and supplemented. Zhytomyr: Polissya.2015:286.