Studying fatty acid content in red blood cell membranes in diabetic retinopathy of type 2 diabetes patients

Mykhailo Bykhovets

DOI: http://dx.doi.org/10.12775/JEHS.2020.10.10.005

Abstract


Introduction. Studying pathogenetic pathways that are formed under conditions of hyperglycaemia and trigger further metabolic changes resulting in diabetes complications over time are of special interest both in theoretical and practical medicine. Membrane of the red blood cell that stays in blood constantly and relatively durable is an obvious participant of all metabolic changes and cannot but reflect changes in metabolism. Objective: to study content of fatty acids (FAs) in red blood cell membranes of patients with diabetic retinopathy (DR) with type 2 diabetes (T2D). Materials and methods. The study enrolled 73 subjects maximally matched by age and gender: 50 patients with established long-lasting T2D complicated with DR and 23 patients in the control group. All the biochemical tests were performed at the certified laboratory under the standard methods. Gas chromatography was applied to study FA spectrum. Statistical analysis was performed in IBM SPSS Statistics 23. Results and discussion. The key difference between the lipid metabolism of patients with complicated long-lasting T2D and healthy subjects was a significant difference in FA content redistribution in the red blood cell membranes represented by increased “saturation”. The content of saturated FAs (SAFAs)in patients with DR was higher than in the CG: 1.5-fold for palmitic (С:16), 2-fold for myristic(С:14), pentadecanoic (С:15) and margaric (С:17) (Р < 0.05). The content of saturated stearic (С:18) FA was not changed significantly.The content of unsaturated FAs(USFAs) have changed multidirectionally: the content of linoleic (С18:2) and arachidonic (С20:4) decreased 1.5-1.7-fold, respectively. The content of linolenic (С18:3) increased 2-fold, and content of oleic FA (С18:1) was not changed significantly. Conclusion. Study of the fluctuations of FA levels in red blood cell membranesplays animportant role in the development of insulin resistance, T2D and its microvascular complicationsthat can be applied in creating treatment and prevention strategies ofDRin type 2 diabetes patients.


Keywords


glycosilated haemoglobin; red blood cell membranes; type 2 diabetes; diabetic retinopathy

Full Text:

PDF

References


Balashevich L.I., Izmaylov A.S. Diabeticheskaya oftal'mopatiya. SPb: Chelovek; 2012. 396 s. [in Russia]

Pasechnikova N.V. Diabeticheskaya makulopatiya. Sovremennyye aspekty patogeneza, kliniki, diagnostiki, lecheniya. Kiyev: iz-vo OOO «Karbon LTD». 2010. 154 s [in Russia]

International diabetes federation annual report 2015. https://www.oedg.at/pdf/1606_IDF_ Atlas_2015_UK.pdf

Chernobryvtsev O.P. The endothelial dysfunction factors in diabetes mellitus 2 type. Journal of Education, Health and Sport formely of Health Sciences. 2019;9(1):525-533.

Benarous R, Sasongko MB, Qureshi S, et al. Differential association of serum lipids with diabetic retinopathy and diabetic macular edema. Invest Ophthalmol Vis Sci. 2011 Sep 27;52(10):7464-9. doi: 10.1167/iovs.11-7598.

Cetin E. N., Bulgu Y., Ozdemir S., et al. Association of serum lipid levels with diabetic retinopathy. Int J Ophthalmol. 2013; 6(3): 346–349.

Rema M, Srivastava BK, Anitha B, et al. Association of serum lipids with diabetic retinopathy in urban South Indians--the Chennai Urban Rural Epidemiology Study (CURES) Eye Study-2. Diabet Med. 2006 Sep;23(9):1029-36.

Koehrer P. Erythrocyte Phospholipid and Polyunsaturated Fatty Acid Composition in Diabetic Retinopathy/ P. Koehrer, S.Saab, O. Berdeaux //2014 journal.pone 0106912

Petrenko O. V. , Natrus L. V., Tavartkiladze K. Aspects of fatty acid content in blood cells of patients with diabetic retinopathy. Archive of Ukrainian Ophthalmology. – 2017. – Vol. 5, No. 2.

Weijers Rob N.M. Lipid Composition of Cell Membranes and Its Relevance in Type 2 Diabetes Mellitus. Curr Diabetes Rev. 2012 Sep; 8(5): 390–400.

Shin S, Ku Y, Babu N, Singh M. Erythrocyte deformability and its variation in diabetes mellitus. Indian J Exp Biol. 2007 Jan;45(1):121-8.

Hussein J. S. Cell membrane fatty acids and health / J. S. Hussein // Int. J. Pharm. Sci. – 2013. – Vol. 5, suppl. 3. – P. 38–46.

Boden G, Chen X and Iqbal N. Acute lowering of plasma fatty acids lowers basal insulin secretion in diabetic and nondiabetic subjects. Diabetes 1998 Oct; 47(10): 1609-1612.https://doi.org/10.2337/diabetes.47.10.1609

Boden G, Laakso M. Lipids and Glucose in Type 2 Diabetes. What is the cause and effect? Diabetes Care 2004 Sep; 27(9): 2253-2259. https://doi.org/10.2337/diacare. 27.9.2253

Benjamin SM, Valdez R, Geiss LS, Rolka DB, Narayan KM: Estimated number of adults with prediabetes in the US in 2000: opportunities for prevention. Diabetes Care 26:645–649, 2003


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM




Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska, EuroPub database, NLM Catalog Result - NCBI, BASE, Russian Sciences Index, Arianta.

https://zdb-katalog.de/title.xhtml?idn=1080187162

https://europub.co.uk/journals/22625

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Publisher and Editorial Office
Department of Physical Culture,
Faculty of Earth Sciences and Spatial Management,
Nicolaus Copernicus University in Toruń, Poland
Address: Str. Lwowska 1, 87-100 Toruń, Poland

  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

RG Journal Impact: 0.18 *

*This value is calculated using ResearchGate data and is based on average citation counts from work published in this journal. The data used in the calculation may not be exhaustive.

RG Journal impact history

2020Available summer 2021
2018 / 20190.18
20170.07
20160.03

RG Journal impact over time

RG Journal impact
0.180.160.140.120.100.080.060.040.02201620172018
  

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)

Partnerzy platformy czasopism