The effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the apoptosis of pulmonary leukocytes and its correction by carbacetam

O. V. Stakhiv, A. A. Hudyma

DOI: http://dx.doi.org/10.12775/JEHS.2020.10.09.117

Abstract


The development of acute lung injury is one of the severe complications of massive blood loss. Under these conditions, the apoptosis which is identified as an informative marker of the severity of the pathological process, enhances in the pulmonary leukocytes. The effect of limb ischemia and its reperfusion on the systemic mechanisms of the pathogenesis of acute massive blood loss, in particular the level of apoptosis of pulmonary leukocytes, remains understudied. There are no data concerning the efficacy of the carbacetam under these conditions.

The objective of the research: to establish the effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the early apoptosis of pulmonary leukocytes and the efficacy of the carbacetam in the correction of identified abnormalities.

Material and methods. The experimental studies were conducted on 216 white non-linear male rats weighing 200-220g. All procedures were carried out under thiopental sodium anesthesia. A limb ischemia-reperfusion, an acute blood loss as well as a combination of these lesions were carried out in the experimental groups. The identified abnormalities were corrected with carbacetam in the separate experimental group. After 1 and 2 hours as well as 1, 7, and 14 days, the bronchoalveolar lavage fluid of experimental animals was taken for the detection of the level of early apoptosis in the leukocyte population.

The results. The simulations of a limb ischemia-reperfusion, an acute blood loss, and a combination of these lesions create the preconditions for the enhancement of early apoptosis of pulmonary leukocytes obtained from the bronchoalveolar lavage fluid. If under the conditions of simulating, solely, the limb ischemia-reperfusion the index is significantly higher than the control after 1 hour, 2 hours, and 1 day, and returns to the control level after 7-14 days, then after simulation an acute blood loss and its complication by limb ischemia-reperfusion the index is statistically significantly higher than control level at all observation periods. It has been established that the combination of massive acute blood loss and limb ischemia-reperfusion is accompanied by statistically significantly great increase in the studied parameter, as compared to the other experimental groups. The index statistically significantly decreases in all experimental groups under the effect of the carbacetam, which indicates the marked proapoptotic effect of the medication. Notwithstanding that studied parameters do not reach the level of the control group, the carbacetam is considered to be the promising medication for the correction of acute lung injury under conditions of acute blood loss complicated by limb ischemia-reperfusion.

Conclusions. The simulation of a limb ischemia-reperfusion, an acute blood loss, and a combination of these lesions create the preconditions for the enhancement of early apoptosis of pulmonary leukocytes obtained from the bronchoalveolar lavage fluid. The complication of acute blood loss by limb ischemia and reperfusion is followed by statistically significantly greater abnormalities at all observation periods. Administration of the carbacetam leads to decrease in the level of early apoptosis in the bronchoalveolar lavage leukocytes after 7-14 days, which indicates the prospects of the medication for correction of identified abnormalities.


Keywords


acute blood loss; limb ischemia-reperfusion; early apoptosis; leukocytes; bronchoalveolar lavage (BAL), carbacetam

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References


Kozak D. V. Porushennia protsesiv lipidnoi peroksydatsii ta antyoksydantnoho zakhystu u tkanyni lehen v piznii period travmatychnoi khvoroby ta yikh korektsiia. Visnyk nauk. doslidzhen. 2015. № 3. P. 101 – 103.

Marushchak M.I. Rol neitrofilnoho apoptozu v patohenzi eksperymentalnoho HCl-indukovanoho hostroho urazhennia lehen. Zahalna patolohiia ta patolohichna fiziolohiia. 2012. T. 7, № 1. P. 74-79.

Shatskyi V. V., Hudyma A. A., Blyzniuk R. V. Vplyv eksperymentalnoi hostroi krovovtraty, uskladnenoi ishemiieiu-reperfuziieiu kintsivky, na diurez i shvydkist klubochkovoi filtratsii. [Influence of experimental acute blood loss complicated by ischemia-reperfusion of the extremity on the size of diuresis and the glomerular filtration rate] Shpytalna khirurhiia. Zhurnal imeni L. Ya. Kovalchuka [Hospital Surgery. Journal named by L.Ya. Kovalchuk]. 2019. № 4. P. 50–58.

Abraham E. Neutrophils and acute lung injury. Critical Care Medicine. 2003. Vol. 31, № 4. P. 195—199.

Comparison of changes in markers of muscle damage induced by eccentric exercise and ischemia/reperfusion / Q. S. Su, J. G. Zhang, R. Dong [et al.]. Scand. J. Med. Sci. Sports. 2010. Vol. 20, No. 5. Р. 748–756.

Concentration decrease of nitric oxide in the postischemic muscle is not only caused by the generation of O2- / F. Stoffels, F. Lohöfener, М. Beisenhirtz [et al.]. Microsurgery. 2007. Vol. 27, No. 6. Р. 565–568.

Incidence and Outcomes of Acute Lung Injury / G. D. Rubenfeld, E. Caldwell, E. Peabody [et al.]. New Engl. J. Med. 2005. Vol. 353, № 5. P. 1685–1693.

Maianski N. A., Maianski A. N., Kuijpers T. W., Roos D. Apoptosis of neutrophils. Acta Haematol. 2004. Vol. 111, № 1-2. P. 56–66.

Peculiarities of impairments of absorptive-excretory and glycogen synthesis functions of the liver in the presence of acute blood loss complicated by limb ischemia-reperfusion and their correction by carbacetam / I. I. Horban, A. A. Hudyma, R. V. Maksymiv, N. A. Dzhavadova. Journal of Education, Health and Sport. 2020. 10(5). Р. 301-314.

Point of injury tourniquet application during Operation Protective Edge-What do we learn? / A. Shlaifer, A. Yitzhak, E. N. Baruch [et al.]. J. Trauma Acute Care Surg. 2017. Vol. 83, Issue 2. P. 278–283.

Shatskyi V. V., Hudyma A. A. Significance of lipid peroxidation processes in renal excretory dysfunction in cases of acute blood loss complicated by limb ischemia-reperfusion and its correction. Journal of Education, Health and Sport. 2019. Vol.9, № 11. Р. 293–306.

Soubani A. M. O. Acute respiratory distress syndrome: a clinical update / A. M. O. Soubani, R. Pieroni. South Med. J. 1999. Vol. 92. Р. 450–457.

Yang K. Y. Early alterations in neutrophil activation are associated with outcome in acute lung injury / K. Y. Yang, J. J. Arcaroli, E. Abraham. Am. J. Respir. Crit. Care Med. 2003. Vol. 167, № 11. P. 1567–1574.


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