Humanities
Skip to main content Skip to main navigation menu Skip to site footer
  • Register
  • Login
  • Menu
  • Home
  • Current
  • Archives
  • Announcements
  • About
    • About the Journal
    • Submissions
    • Editorial Team
    • Privacy Statement
    • Contact
  • Register
  • Login

Journal of Education, Health and Sport

The effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the apoptosis of pulmonary leukocytes and its correction by carbacetam
  • Home
  • /
  • The effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the apoptosis of pulmonary leukocytes and its correction by carbacetam
  1. Home /
  2. Archives /
  3. Vol. 10 No. 9 (2020) /
  4. Research Articles

The effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the apoptosis of pulmonary leukocytes and its correction by carbacetam

Authors

  • O. V. Stakhiv Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine
  • A. A. Hudyma Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

DOI:

https://doi.org/10.12775/JEHS.2020.10.09.117

Keywords

acute blood loss, limb ischemia-reperfusion, early apoptosis, leukocytes, bronchoalveolar lavage (BAL), carbacetam

Abstract

The development of acute lung injury is one of the severe complications of massive blood loss. Under these conditions, the apoptosis which is identified as an informative marker of the severity of the pathological process, enhances in the pulmonary leukocytes. The effect of limb ischemia and its reperfusion on the systemic mechanisms of the pathogenesis of acute massive blood loss, in particular the level of apoptosis of pulmonary leukocytes, remains understudied. There are no data concerning the efficacy of the carbacetam under these conditions.

The objective of the research: to establish the effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the early apoptosis of pulmonary leukocytes and the efficacy of the carbacetam in the correction of identified abnormalities.

Material and methods. The experimental studies were conducted on 216 white non-linear male rats weighing 200-220g. All procedures were carried out under thiopental sodium anesthesia. A limb ischemia-reperfusion, an acute blood loss as well as a combination of these lesions were carried out in the experimental groups. The identified abnormalities were corrected with carbacetam in the separate experimental group. After 1 and 2 hours as well as 1, 7, and 14 days, the bronchoalveolar lavage fluid of experimental animals was taken for the detection of the level of early apoptosis in the leukocyte population.

The results. The simulations of a limb ischemia-reperfusion, an acute blood loss, and a combination of these lesions create the preconditions for the enhancement of early apoptosis of pulmonary leukocytes obtained from the bronchoalveolar lavage fluid. If under the conditions of simulating, solely, the limb ischemia-reperfusion the index is significantly higher than the control after 1 hour, 2 hours, and 1 day, and returns to the control level after 7-14 days, then after simulation an acute blood loss and its complication by limb ischemia-reperfusion the index is statistically significantly higher than control level at all observation periods. It has been established that the combination of massive acute blood loss and limb ischemia-reperfusion is accompanied by statistically significantly great increase in the studied parameter, as compared to the other experimental groups. The index statistically significantly decreases in all experimental groups under the effect of the carbacetam, which indicates the marked proapoptotic effect of the medication. Notwithstanding that studied parameters do not reach the level of the control group, the carbacetam is considered to be the promising medication for the correction of acute lung injury under conditions of acute blood loss complicated by limb ischemia-reperfusion.

Conclusions. The simulation of a limb ischemia-reperfusion, an acute blood loss, and a combination of these lesions create the preconditions for the enhancement of early apoptosis of pulmonary leukocytes obtained from the bronchoalveolar lavage fluid. The complication of acute blood loss by limb ischemia and reperfusion is followed by statistically significantly greater abnormalities at all observation periods. Administration of the carbacetam leads to decrease in the level of early apoptosis in the bronchoalveolar lavage leukocytes after 7-14 days, which indicates the prospects of the medication for correction of identified abnormalities.

References

Kozak D. V. Porushennia protsesiv lipidnoi peroksydatsii ta antyoksydantnoho zakhystu u tkanyni lehen v piznii period travmatychnoi khvoroby ta yikh korektsiia. Visnyk nauk. doslidzhen. 2015. № 3. P. 101 – 103.

Marushchak M.I. Rol neitrofilnoho apoptozu v patohenzi eksperymentalnoho HCl-indukovanoho hostroho urazhennia lehen. Zahalna patolohiia ta patolohichna fiziolohiia. 2012. T. 7, № 1. P. 74-79.

Shatskyi V. V., Hudyma A. A., Blyzniuk R. V. Vplyv eksperymentalnoi hostroi krovovtraty, uskladnenoi ishemiieiu-reperfuziieiu kintsivky, na diurez i shvydkist klubochkovoi filtratsii. [Influence of experimental acute blood loss complicated by ischemia-reperfusion of the extremity on the size of diuresis and the glomerular filtration rate] Shpytalna khirurhiia. Zhurnal imeni L. Ya. Kovalchuka [Hospital Surgery. Journal named by L.Ya. Kovalchuk]. 2019. № 4. P. 50–58.

Abraham E. Neutrophils and acute lung injury. Critical Care Medicine. 2003. Vol. 31, № 4. P. 195—199.

Comparison of changes in markers of muscle damage induced by eccentric exercise and ischemia/reperfusion / Q. S. Su, J. G. Zhang, R. Dong [et al.]. Scand. J. Med. Sci. Sports. 2010. Vol. 20, No. 5. Р. 748–756.

Concentration decrease of nitric oxide in the postischemic muscle is not only caused by the generation of O2- / F. Stoffels, F. Lohöfener, М. Beisenhirtz [et al.]. Microsurgery. 2007. Vol. 27, No. 6. Р. 565–568.

Incidence and Outcomes of Acute Lung Injury / G. D. Rubenfeld, E. Caldwell, E. Peabody [et al.]. New Engl. J. Med. 2005. Vol. 353, № 5. P. 1685–1693.

Maianski N. A., Maianski A. N., Kuijpers T. W., Roos D. Apoptosis of neutrophils. Acta Haematol. 2004. Vol. 111, № 1-2. P. 56–66.

Peculiarities of impairments of absorptive-excretory and glycogen synthesis functions of the liver in the presence of acute blood loss complicated by limb ischemia-reperfusion and their correction by carbacetam / I. I. Horban, A. A. Hudyma, R. V. Maksymiv, N. A. Dzhavadova. Journal of Education, Health and Sport. 2020. 10(5). Р. 301-314.

Point of injury tourniquet application during Operation Protective Edge-What do we learn? / A. Shlaifer, A. Yitzhak, E. N. Baruch [et al.]. J. Trauma Acute Care Surg. 2017. Vol. 83, Issue 2. P. 278–283.

Shatskyi V. V., Hudyma A. A. Significance of lipid peroxidation processes in renal excretory dysfunction in cases of acute blood loss complicated by limb ischemia-reperfusion and its correction. Journal of Education, Health and Sport. 2019. Vol.9, № 11. Р. 293–306.

Soubani A. M. O. Acute respiratory distress syndrome: a clinical update / A. M. O. Soubani, R. Pieroni. South Med. J. 1999. Vol. 92. Р. 450–457.

Yang K. Y. Early alterations in neutrophil activation are associated with outcome in acute lung injury / K. Y. Yang, J. J. Arcaroli, E. Abraham. Am. J. Respir. Crit. Care Med. 2003. Vol. 167, № 11. P. 1567–1574.

Downloads

  • PDF

Published

2020-09-30

How to Cite

1.
STAKHIV, O. V. and HUDYMA, A. A. The effect of acute blood loss complicated by limb ischemia-reperfusion on the induction of the apoptosis of pulmonary leukocytes and its correction by carbacetam. Journal of Education, Health and Sport. Online. 30 September 2020. Vol. 10, no. 9, pp. 978-987. [Accessed 5 July 2025]. DOI 10.12775/JEHS.2020.10.09.117.
  • ISO 690
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
Download Citation
  • Endnote/Zotero/Mendeley (RIS)
  • BibTeX

Issue

Vol. 10 No. 9 (2020)

Section

Research Articles

License

The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0

Stats

Number of views and downloads: 487
Number of citations: 0

Search

Search

Browse

  • Browse Author Index
  • Issue archive

User

User

Current Issue

  • Atom logo
  • RSS2 logo
  • RSS1 logo

Information

  • For Readers
  • For Authors
  • For Librarians

Newsletter

Subscribe Unsubscribe

Tags

Search using one of provided tags:

acute blood loss, limb ischemia-reperfusion, early apoptosis, leukocytes, bronchoalveolar lavage (BAL), carbacetam
Up

Akademicka Platforma Czasopism

Najlepsze czasopisma naukowe i akademickie w jednym miejscu

apcz.umk.pl

Partners

  • Akademia Ignatianum w Krakowie
  • Akademickie Towarzystwo Andragogiczne
  • Fundacja Copernicus na rzecz Rozwoju Badań Naukowych
  • Instytut Historii im. Tadeusza Manteuffla Polskiej Akademii Nauk
  • Instytut Kultur Śródziemnomorskich i Orientalnych PAN
  • Instytut Tomistyczny
  • Karmelitański Instytut Duchowości w Krakowie
  • Ministerstwo Kultury i Dziedzictwa Narodowego
  • Państwowa Akademia Nauk Stosowanych w Krośnie
  • Państwowa Akademia Nauk Stosowanych we Włocławku
  • Państwowa Wyższa Szkoła Zawodowa im. Stanisława Pigonia w Krośnie
  • Polska Fundacja Przemysłu Kosmicznego
  • Polskie Towarzystwo Ekonomiczne
  • Polskie Towarzystwo Ludoznawcze
  • Towarzystwo Miłośników Torunia
  • Towarzystwo Naukowe w Toruniu
  • Uniwersytet im. Adama Mickiewicza w Poznaniu
  • Uniwersytet Komisji Edukacji Narodowej w Krakowie
  • Uniwersytet Mikołaja Kopernika
  • Uniwersytet w Białymstoku
  • Uniwersytet Warszawski
  • Wojewódzka Biblioteka Publiczna - Książnica Kopernikańska
  • Wyższe Seminarium Duchowne w Pelplinie / Wydawnictwo Diecezjalne „Bernardinum" w Pelplinie

© 2021- Nicolaus Copernicus University Accessibility statement Shop