Immunohistochemical studies on lymphocyte subpopulation in endometrium samples in women with infertility
DOI:
https://doi.org/10.12775/JEHS.2020.10.06.002Keywords
endometriosis, infertility, natural killer cells, thin endometrium, endometrial hyperplasiaAbstract
Uterine leucocytes have been thought to play a key role in endometrial pathology and infertility.
The aim of this study was to compare the amount of CD20+, CD3+, CD56+, CD138+ cells in the endometrium from women with simple endometrial hyperplasia without atypia and thin endometrium with the presence of infertility. 95 patients, with simple endometrial hyperplasia without atypia, thin endometrium, and women without endometrial pathology (controls) were selected as research subjects. Hematoxylin and eosin staining were performed on all endometrial biopsies. Immunohistochemical staining for CD20+, CD3+, CD56+, CD138+ cells was applied to all tissue samples. Additionally, the disease histories of patients were collected, were held clinical and laboratory tests. The number of CD20+ cells was higher in 1 group (4,129±0,55) and 2 group (5,872±0,85), compared to fertile patients (1,353±0,16, p<0.05). The ratio of CD3+ cells was higher in women with endometrial hyperplasia (108,871±14,34) and lower in women with a thin endometrium (36,388±3,83), compared to fertile patients (72,848±6,11, p<0.05). The study shows a notable increase of CD138+ in specimens with hyperplasia (19,18±1,2) and thin endometrium (20,81±1.45) compared to fertile patients (1,02±0,5, p<0.05 in both cases), and decreased of CD56-positive cells in stroma (43,4±2,2; 33.6±2.8) and in glands (7,4±1,2; 3.6±1,8) for hyperplasia and thin endometrium respectively compared to control group (51,2±2,5; 14,8±0,9). Women, with endometrial hyperplasia, have larger populations of CD20+, CD3+, and CD138+ and decreased amount of CD56+ in the stroma and glandular cells, as for women with thin endometrium, they have large populations of CD20+ and CD138+ and small of CD3+, CD56+, but bough groups may be at greater risk for infertility disorders resulting from an inflammatory environment occurring during implantation or later during decidualization.
References
Bulmer JN, Williams PJ, Lash GE. Immune cells in the placental bed, Int. J. Dev. Biol. 2010;54:281-294. DOI: 10.1387/ijdb.082763jb.
Kitaya K., Tada Y., Hayashi T, Taguchi S., Funabiki M., Nakamura Y. Comprehensive endometrial immunoglobulin subclass analysis in infertile women suffering from repeated implantation failure with or without chronic endometritis. Am J. Reprod Immunol. 2014;72:386– 391. DOI: 10.1111/aji.12277
Kitaya K., Tada Y., Taguchi S. Local Mononuclear Cell Infiltrates in Infertile Patients With Endometrial Macropolyps Versus Micropolyps. Hum. Reprod.2012;27: 3474–3480. DOI: 10.1093/humrep/des323.
Kitaya K, Yamaguchi T, Yasuo T, Okubo T, Honjo H: Post‐ovulatory rise of endometrial CD16(‐) natural killer cells: in situ proliferation of residual cells or selective recruitment from circulating peripheral blood. J. Reprod Immunol.2007;76:45– 53. DOI: 10.1016/j.jri.2007.03.010.
Kitaya K., Yasuo T. Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis. Mod. Pathol. 2010;23:1136– 1146. DOI: 10.1038/modpathol.2010.98.
Kitaya K., Yasuo T. Inter-observer and intra-observer variability in immunohistochemical detection of endometrial stromal plasmacytes in chronic endometritis. Experimental and therapeutic medicine. 2013;5(2):485-488. DOI: 10.3892/etm.2012.824
Kitaya K., Yoshihiro T., Sagiri T. Miyako F., Terumi H., Yoshitaka N. Local mononuclear cell infiltrates in infertile patients with endometrial macropolyps versus micropolyps. Human reproduction (Oxford, England). 2012;27: 3474–3480. DOI: 10.1093/humrep/des323
Klentzeris LD, Bulmer JN, Warren MA, Morrison L, Li TC, Cooke ID. Lymphoid tissue in the endometrium of women with unexplained infertility: morphometric and immunohistochemical aspects. Hum Reprod. 1994;47:646–652. DOI: 10.1093/oxfordjournals.humrep.a138564.
Kuon RJ, Vomstein K, Weber M. The “killer cell story” in recurrent miscarriage: Association between activated peripheral lymphocytes and uterine natural killer cells. J. Reproductive Immunology. 2016;11:123-134. DOI: 10.1016/j.jri.2016.11.002.
Lachapelle MH, Miron P, Hemmings R, Roy DC. Endometrial T, B, and NK cells in patients with recurrent spontaneous abortion. Altered profile and pregnancy outcome. J Immunol., 1996;156(10):4027–4034.
Lucas ES, Dyer NP, Murakami K, Hou Lee Y, Chan YW, Grimaldi G, Muter J, Brighton PJ, Moore JD, Patel G, Chan JKY, Takeda S, Lam EWF, Quenby S, Ott S, Brosens JJ. Loss of endometrial plasticity in recurrent pregnancy loss. Stem Cells., 2016;34( 2): 346–356. DOI: 10.1002/stem.2222.
Mamedaliyeva N. M., Kurmanova A. M., Lokshin V. N., Kurmanova G. M. ,. Issenova S. S. Clinical and immunological parallels in pregnancy loss. Gynecological Endocrinology., 2017;33(1):5-7. DOI: 10.1080/09513590.2017.1404238.
Menaldo G., Uterine Natural Killer Cells In Proliferative Endometrium Of Infertile Women. The World Congress on Controversies in Obstetrics, Gynaecology and Infertility, Morressier 2019
Van Mourik MS, Macklon NS, Heijnen CJ. Embryonic implantation: cytokines, adhesion molecules, and immune cells in establishing an implantation environment. J Leukoc Biol., 2009;85(1):4–19. DOI: 10.1189/jlb.0708395.
Yeaman GR, Guyre PM, Fanger MW, Collins JE, White HD, Rathbun W, Orndorff KA, Gonzalez J, Stern JE, Wira CR: Unique CD8+ T cell‐rich lymphoid aggregates in human endometrium. J Leukoc Biol., 1997;61: 427– 435.
Downloads
Published
How to Cite
Issue
Section
License
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 720
Number of citations: 0
