Ultrastructural features of blood cells in HIF-1α gene variations in specialists of extreme conditions
DOI:
https://doi.org/10.12775/JEHS.2020.10.05.023Keywords
allelic polymorphism, the HIF-1α gene, platelet and leukocyte ultrastructure, hypoxic load, winterers of Antarctic expeditionsAbstract
The peculiarities of changes in the ultrastructure of blood cells due to personalized variations of the HIF-1α gene under hypoxic loading (breathing by gas mixture with reduced oxygen content up to 12% and achieving hypoxic state during heavy physical training on bicycle ergometer) were conducted.
It was shown that allelic polymorphism of the HIF-1α gene influence on hypoxic-induced changes in the ultrastructure (and therefore function) of blood cells - the C / C genotype of winterers indicates their greater adaptive capacity compared to the C / T genotype that lies in the increasing the number of δ-granules in platelets, and reducing the number of α-granules and tubules of the inner skeleton. Such changes primarily indicate an increase in serotonin depot and therefore, indirectly, an increase in the contractile activity of the vascular wall, which may serve as a marker of the formation of adaptive reaction in response to hypoxic effects and stress. This is also indicated by the moderate swelling of mitochondria with an increase in their average diameter by 31%, which is considered to be a sign of an increase in the energy capacity of the mitochondrial apparatus in order to replenish ADP and ATP reserves for the further inclusion of adaptive responses. Hypoxic loading in winterers with C / T-genotype led to overstressing of platelet function with decrease in contractile activity of the vascular wall.
The number of mitochondria in leukocytes significantly increased (by 24%) in winterers with C / C genotype; not only mitochondrial morphogenesis was activated, but also dynamic processes, in particular the association of organelles with pronounced longitudinal association and / or the formation of bridges between mitochondria. These facts are considered as an indicator of information exchange in the cell mitochondrial apparatus. In leukocytes of winterers with C / T genotype, changes in the mitochondrial apparatus were of a different nature: there were clear signs of hypoxic damage of organelles and the total percentage of structurally altered mitochondria exceeded 21%.
References
Bakhchevan EL, Chebotar SV Molecular-genetic markers of adaptation to hypoxia in sportsmen. Bul ONU. Biology. 2015; 20 (1): 69-81. Russian.
Bruick RK Conserved family of ptolyl-4-hydroxylases that modify HIF. Science. 2001; 294 (5545): 1337–40. DOI: 10.1126/science.1066373
Chavez JC Expression of hypoxia-inducible factor-1 in the brain of rats during chronic hypoxia. J Appl Physiol. 2000; 89 (5): 1937–42. DOI: 10.1152/jappl.2000.89.5.1937
Moiseyenko YV, Sukhorukov VI, Pyshnov GYu [et al.]. Antarctica challenges the new horizons in predictive, preventive, personalized medicine: preliminary results and attractive hypothesis for multi-disciplinary prospective studies in the Ukrainian `Akademik Vernadsky` Station. EPMA J. 2016; 45. DOI: 10.1186/s13167-016-0060-8.
Akhmetov II, Khakimullina AM, Lyubaeva EI [et al.]. Effect of HIF-1α gene polymorphism on human muscle activity. Bul. Experim. Biol. Med. 2008; 146 (9): 327-9. DOI: 10.1007/s10517-008-0291-3. Russian.
Drevitskaya TI Expression of α-subunits of the hypoxia-inducible factor (HIF) gene, its allelic polymorphism under normoxic and hypoxic conditions: Abstract diss. ... cand. biol. sci. 03.00.13. - Kyiv, 2010. - 24 p.
Döring F, Onur S, Ficher A [et al.]. A common haplotype and Pro582Ser polymorphism of the hypoxia− inducible factor-1α (HIF1A) gene in elite endurance athletes. J Appl Physiol. 2010; 108 (6) – P.1497− 500. https://doi.org/10.1152/japplphysiol.01165.2009
Poptsov V. Some aspects of sports physiology as applied to endurance sports. Cross-country skiing. 1998; (1): C.3−8. Russian.
Tanimoto K, Yoshiga K, Eguchi H [et al.]. Hypoxia−inducible factor − 1[alpha] polymorphisms associated with enhanced transactivation capacity, implying clinical significance. Carcinogenesis. 2003; 24 (11): 1779− 83. DOI: 10.1093/carcin/bgg132
Cieszczyk P, Eider J, Arczewska A [et al.]. The HIF1A gene Pro582Ser polymorphism in polish power−orientated athletes. Biology of sport. 2011; 28 (2): 111− 4. DOI: 10.5604/945117
Prior SJ, Hagberg JM, Phares DA [et al.]. Sequence variation in hypoxia-inducible factor 1α (HIF1A): association with maximal oxygen consumption. Physiol Genomics. 2003; 15 (1): 20–6. DOI:10.1152/physiolgenomics.00061.2003
Bohuslavovа R, Kolаr F, Kuthanov L [et al.]. Gene expression profiling of sex differences in HIF1− dependent adaptive cardiac responses to chronic hypoxia. J Appl Physiol. 2010; 109 (4): 1195–202. DOI: 10.1152/japplphysiol.00366.2010
Mankovska IM, Moiseyenko EV, Dyachenko MP [et al.]. Peculiarities of the awakening of the functional system of dying after the trivial awakening in the minds of the Antarctic. Fiziol. Zh. 2005; 51 (3): 25−31. Ukrainian.
Bubnov RV., Moiseyenko YeV., Spivak MYa. The influence of environmental factors and stress on human health and chronic diseases: PPPM lessons from Antarctica. EPMA Journal. 2017: 13-23. DOI: 10.1007/s13167-017-0108-4
Filippova OI, Koloskov AV, Capital AA. Methods for the study of the functional activity of platelets (literature review). Transfusology. 2012; 13 (2): 493-514. Russian.
Karupu VYa. Elektronnaya mikroskopiya [Electron microscopy]. Kiyv. Vyshcha shkola, 1984; 208. Russian.
Lukyanova EM, Antipkin Yu. G., Chernyshov VP, Vykhovanets EV Metodika statisticheskoy obrabotki meditsinskoy informatsii v nauchnykh issledovaniyakh [Methods of statistical processing of medical information in scientific research]. Kiyv. Planeta lyudey, 2002; 200. Russian.
Kolosova EN, Vasilenko IA, Kovaleva LG. Evaluation of the morphological and functional state of platelets in patients with idiopathic thrombocytopenic purpura by vital computer morphometry. Bull. SB RAMS. 2011; 31 (2): 58-63. Russian.
Solodovnikova IM, Saprunova VB, Bakeeva LE, Yaguzhinsky LS. Dynamics of changes in the mitochondrial ultrastructure of cardiomyocytes of an isolated rat myocardium during prolonged incubation under anoxia. Cytology. 2006; 48 (10): 848-55. Russian.
Mironova GD, Rozova EV, Belosludtseva NV, Man’kovskaya IN. Dynamic Restructuring of the Myocardial Mitochondria in Response to Uridine Modulation of the Activity of Mitochondrial ATP-Dependent Potassium Channel under Conditions of Acute Hypoxic Hypoxia. Bull. Experim. Boil. Med. 2019; 166 (6): 806-10. DOI: 10.1007/s10517-019-04445-9
Downloads
Published
How to Cite
Issue
Section
License
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 570
Number of citations: 0
