Effects of dexamethasone with sodium chloride load on the nitrite anion content in the rat’s blood serum, heart atria and ventricles

Yu. A. Sverediuk

DOI: http://dx.doi.org/10.12775/JEHS.2020.10.01.023


The consequence of prolonged treatment with corticosteroids is development of hypertension and metabolic disorders. On the other hand, high levels of sodium chloride in the diet is able to amplify their risks. In a result of that disorders may be development metabolic cardiomyopathy. One of mechanisms that is capable to preventing hypertension and metabolic disorders is nitric oxide system. The research of that system, account short lifetime of the nitric oxide and features its metabolism, can be performed by analysis of nitrite anion (NO2-). It is mainly inactive metabolites in a result of the molecules NO. L-carnitine, as an antioxidant and having the ability to influence to energic metabolism, apoptosis and transcription of DNA may be a promising means for correction  disorders in the nitric oxide system. The purpose of our study was to investigate the NO2- levels in the animals serum and heart tissue of both  sex for long-term action of dexamethasone in conditions of high concentration of 4 % solution sodium chloride in water and  use L-carnitine in order to correction data. The experiment was performed with the use of 96 mature white nonlinear rats. It was established that long-term use and high salt content and especially their combination  conduced reduce of the NO2- level in the serum and ventricular myocardium. The high changes dominanced in the males. L-carnitine demonstrates the ability to recover the metabolite level. The characterised changes occur between both  sex in animals with intake of usual quantity sodium chloride in water. The usage water with high content of sodium chloride caused more intensive negative changes but the difference between the sex was not as pronounced. The difference of content NO2- in the atria was found only between males and females. The dexamethasone or sodium chloride didn’t exert influence of the NO2- metabolism in that tissue.


NO2-; dexamethasone; sodium chloride load; L-carnitine

Full Text:



Al-Eisa RA, Al-Salmi FA, Hamza RZ, El-Shenawy NS. Role of L-carnitine in protection against the cardiac oxidative stress induced by aspartame in Wistar albino rats. Plos One. 2018Jul; 13 (11): 1-12.

Bae EH, Ma SK, Lee J, Kim SW. Altered regulation of renal nitric oxide and atrial natriuretic peptide systems in angiotensin II-induced hypertension. Regulatory Peptides. 2011; 170 (1-3): 31.07.

Bongartz LG, Braam B, Verhaar MC, Cramer MJ, Goldschmeding R, Gaillard CA, et al. Transient nitric oxide reduction induces permanent cardiac systolic dysfunction and worsens kidney damage in rats with chronic kidney disease. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2010; 298 (3): 15-23.

Bongartz LG, Soni S, Cramer MJ, Steendijk P, Gaillard CA, Verhaar MC, et al. Neuronal Nitric Oxide Synthase-Dependent Amelioration of Diastolic Dysfunction in Rats with Chronic Renocardiac Syndrome. Cardiorenal Medicine. 2015; 5 (1): 69-78.

Couto GK, Britto LRG, Mill JG, Rossoni LV. Enhanced nitric oxide bioavailability in coronary arteries prevents the onset of heart failure in rats with myocardial infarction. Journal of Molecular and Cellular Cardiology. 2015; 86: 110-20.

Drexler H, Kästner S, Strobel A, Studer R, Brodde OE, Hasenfuß G. Expression, activity and functional significance of inducible nitric oxide synthase in the failing human heart. Journal of the American College of Cardiology. 1998; 32 (4): 955-63.

Goldberg JF, Mery CM, Griffiths PS, Parekh DR, Welty SE, Bronicki RA, et al. Extracorporeal Membrane Oxygenation Support in Severe Hypertrophic Obstructive Cardiomyopathy Associated With Persistent Pulmonary Hypertension in an Infant of a Diabetic Mother. Circulation. 2014; 130 (21): 1923-5.

Guimarães FDS, Wilson Max Almeida Monteiro De Moraes, Bozi LHM, Souza PR, Antonio EL, Bocalini DS, et al. Dexamethasone-induced cardiac deterioration is associated with both calcium handling abnormalities and calcineurin signaling pathway activation. Molecular and Cellular Biochemistry. 2016; 424 (1-2): 87-98.

Ito D, Ito O, Mori N, Cao P, Suda C, Muroya Y, et al. Exercise training upregulates nitric oxide synthases in the kidney of rats with chronic heart failure. Clinical and Experimental Pharmacology and Physiology. 2013; 40 (9): 617-25.

Jiang J, Zhang J, Kang M, Yang J. Transient hypertrophic cardiomyopathy and hypertension associated with hydrocortisone in preterm infant. Medicine. 2019; 98 (33): 1-4.

Korhonen R, Lahti A, Hämäläinen M, Kankaanranta H, Moilanen E. Dexamethasone Inhibits Inducible Nitric-Oxide Synthase Expression and Nitric Oxide Production by Destabilizing mRNA in Lipopolysaccharide-Treated Macrophages. Molecular Pharmacology. 2002Jan; 62 (3): 698-704.

Kunz D, Walker G, Eberhardt W, Pfeilschifter J. Molecular mechanisms of dexamethasone inhibition of nitric oxide synthase expression in interleukin 1 beta-stimulated mesangial cells: evidence for the involvement of transcriptional and posttranscriptional regulation. Proceedings of the National Academy of Sciences. 1996Sep; 93 (1): 255-9.

Lee J, Bae EH, Ma SK, Kim SW. Altered Nitric Oxide System in Cardiovascular and Renal Diseases. Chonnam Medical Journal. 2016; 52 (2): 81-90.

Maneesai P, Bunbupha S, Potue P, Berkban T, Kukongviriyapan U, Kukongviriyapan V, et al. Hesperidin Prevents Nitric Oxide Deficiency-Induced Cardiovascular Remodeling in Rats via Suppressing TGF-β1 and MMPs Protein Expression. Nutrients. 2018; 10 (10): 1549.

Paech C, Wolf N, Thome UH, Knüpfer M. Hypertrophic intraventricular flow obstruction after very-low-dose dexamethasone (Minidex) in preterm infants: case presentation and review of the literature. Journal of Perinatology. 2014; 34 (3): 244-6.

Shibata K, Yatera Y, Furuno Y, Sabanai K, Morisada N, Nakata S, et al. Spontaneous Development of Left Ventricular Hypertrophy and Diastolic Dysfunction in Mice Lacking All Nitric Oxide Synthases. Circulation Journal. 2010; 74 (12): 2681-92.

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Journal of Education, Health and Sport formerly Journal of Health Sciences

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1223 Journal of Education, Health and Sport eISSN 2391-8306 7

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