Features of immune status in different states of uric acid metabolism in female rats

Anatoliy Gozhenko, Vadym Smagliy, Inna Korda, Nataliya Badiuk, Walery Zukow, Igor Popovych

DOI: http://dx.doi.org/10.12775/JEHS.2019.09.12.019


Background. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon’s entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functional-metabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.


uricosuria; uricemia; immunity; relationships; female rats.

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Apasov S, Chen JF, Smith P, Sitkovsky M. A2A receptor dependent and A2A receptor independent effects of extracellular adenosine on murine thymocytes in condicion of adenosine deaminase deficiency. Blood. 2000; 95(12): 3859-3867.

Belousova OI, Fedotova MI. Comparative data on changes in spleen, thymus and bone marrow lymphocyte counts in the early post-irradiation period over a wide range of doses [in Russian]. Radiobiology-Radiotherapy. 1968; 9(3): 309-313.

Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.

Carvalho LAC, Lopes JPPB, Kaihami GH, Silva RP, Bruni-Cordoso A, Baldini RL, Meotti FC. Uric acid disrupts hypochlorous acid production and bactericidal activity of HL-60 cells. Redox Biology. 2018; 16: 179-188.

Douglas SD, Quie PG. Investigation of Phagocytes in Disease. Churchil; 1981: 110 p.

El Ridi R, Tallima H. Physiological functions and pathogenic potential of uric acid: A review. J Adv Res. 2017; 8(5): 487-493.

Gao L, Jiang Y, Wang Y, Qu X, Li L, Lou X, Wang Y, GuoW, Liu Y. Male asymptomatic hyperuticemia patients display a lower number of NKG2D+ NK cells before and after a low-purine diet. Medicine (Baltimore). 2018; 97(50): e13668.

Ghaemi-Oskouie F, Shi Yan. The role of uric acid as an endogenous danger signal in immunity and inflammation. Curr Rheumatol Rep. 2011; 13(2): 160-166.

Goryachkovskiy АМ. Clinical Biochemistry [in Russian]. Odesa. Astroprint; 1998: 608 p.

Gozhenko AI. Functional-metabolic continuum [in Russian]. J of NAMS of Ukraine. 2016; 22 (1): 3-8.

Gozhenko AI, Smagliy SS, Korda IV, Zukow W, Popovych IL. Cluster analysis of uric acid exchange parameters in female rats. Journal of Education, Health and Sport. 2019; 9(11): 277-286.

Gozhenko AI, Smagliy SS, Korda IV, Badiuk NS, Zukow W, Popovych IL. Functional relationships between parameters of uric acid exchange and immunity in female rats. Actual problems of transport medicine. 2019; 4 (54): 123–131.

Gozhenko AI, Zukow W, Polovynko IS, Zajats LM, Yanchij RI, Portnichenko VI, Popovych IL. Individual Immune Responses to Chronic Stress and their Neuro-Endocrine Accompaniment. RSW. UMK. Radom. Torun; 2019: 200 p.

Gwang HB, Yang JH, Park TK, Song YB, Hahn JY, Choi JH, Lee JH, Lee SH, Gwon HC, Choi SH. Uric acid level has a U-shaped assotioation with clinical outcomes in patients with vasospastic angina. J Korean Med Sci. 2017; 32(8): 1275-1280/

Henaut L, Candellier A, Boudot S, Grissi M, Mentaverri R, Choukroun G, Brazier M, Kamel S, Massy ZA. New insights into the roles of monocytes/macrophages in cardiovascular calcification associated with chronic kidney disease. Toxins (Basel). 2019; 11(9): 529.

Hoskin DW, Mader JS, Furlong SJ, Conrad DM, Blay J. Inhibition of T cell and NK cell function by adenosine and its contribution to immune evasion by tumor cells (Review). Int J Oncol. 2008; 32(3): 527-535.

Huang S, Apasov S, Koshiba M, SitkovskiM. Role of A2A extracellular adenosine receptor mediated signaling in adenosine mediated inhibition of of T-cell activation and expansion. Blood. 1997; 90(4): 1600-1610.

Ivassivka SV, Popovych IL, Aksentiychuk BI, Flyunt IS. Physiological Activity of Uric Acid and its Role in the Mechanism of Action of Naftussya Water [in Ukrainian]. Kyiv. Computerpress; 2004: 163 p.

Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskwa. Finansy i Statistika; 1989: 78-138.

Kul’chyns’kyi AB, Kovbasnyuk MM, Kyjenko VM., Zukow W, Popovych IL. Neuro-immune relationships at patients with chronic pyelonephrite and cholecystite. Communication 2. Correlations between parameters EEG, HRV and Phagocytosis. Journal of Education, Health and Sport. 2016; 6(10): 377-401.

Morelli M, Carta AR, Kachroo A, Schwarzschild A. Pathophysiological roles for purines: adenosine, caffeine and urate. Prog Brain Res. 2010; 183: 183-208.

Navalta JW, Fedor EA, Schafer MA, Lyons TS, Tibana RA, Pereira GB, Prestes J. Caffeine affects CD8+ lymphocyte differently in naїve and familiar individuals following moderate intensity exercise. Int J Immunopathol Pharmacol. 2016; 29(2): 288-294.

Perederiy VG, Zemskov AM, Bychkova NG, Zemskov VM. Immune status, principles of its evaluation and correction of immune disorders [in Russian]. Kyiv. Zdorovya; 1995: 211 p.

Popadynets’ OO, Gozhenko AI, Zukow W, Popovych IL. Relationships between the entropies of EEG, HRV, immunocytogram and leukocytogram. Journal of Education, Health and Sport. 2019; 9(5): 651-666.

Popovych IL, Kul’chyns’kyi AB, Gozhenko AI, Zukow W, Kovbasnyuk MM, Korolyshyn TA. Interrelations between changes in parameters of HRV, EEG and phagocytosis at patients with chronic pyelonephritis and cholecystitis. Journal of Education, Health and Sport. 2018; 8(2): 135-156.

Sofaer JA, Emery AF. Genes for super-intelligense? J Med Genet. 1981; 18: 410-413.

Vigano S, Alatzoglou D, Irving M, Menetrier-Caux Ch, Caux Ch, Romero P, Coukos G. Targeting adenosine in cancer immunotherapy to enhance T-cell function. Front Immunol. 2019; 10: 925.

Wang Y, Ma X, Su Ch, Peng B, Du J, Jia H, Luo M, Fang Ch, Wei Y. Uric acid enhanced the antitumor immunity of dendritic cell-based vaccine. Sci Rep. 2015; 5: 16427.

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1223 Journal of Education, Health and Sport eISSN 2391-8306 7

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