The influence of combined abdominal and thoracic trauma on the bile excretory function of the liver in the period of early manifestations of traumatic disease and their correction with thiotriazoline

O. A. Zachepa, A. A. Hudyma, Y. I. Sushko

DOI: http://dx.doi.org/10.12775/JEHS.2019.09.12.016

Abstract


Introduction. Traumatism is one of the challenging issues of today's urban society. Multiple and combined lesions, which are characterized by significant severity and high mortality and lead to multiorgan dysfunction and insufficiency, dominate the structure of traumas. In recent years studies of the functional state of the liver have been widely used as a model for development of multiorgan dysfunction in conditions of severe experimental trauma.

Objective of the research: to find out the character of disorders of bile excretory function of the liver in conditions of the combined abdominal and thoracic trauma of rats in the early period of a traumatic disease, and to evaluate the effectiveness of their correction with thiotriazoline.

Materials of the research and their discussion. The experiments were conducted on 86 non-linear white male rats weighing 200-220 g. All animals were divided into five groups: a control and four experimental ones. Injuries were inflicted under thiopental sodium anesthesia. The control group included intact animals, which were only injected into anesthesia. Animals with simulated thorax trauma were in the first experimental group. In the second experimental group a blunt abdominal trauma was simulated. In the third experimental group, these traumas were combined. In the fourth experimental group of animals with a combined trauma, thiotriazoline was administered intraperitoneally at a dose of 9.1 mg per
kg-1. In 1, 3, and 7 days after injuries were caused under conditions of thiopental sodium anesthesia, the common bile duct was catheterized in animals and bile was taken for 60 minutes. The rate of bile excretion was set. The concentration of total bile acids and conjugated bilirubin was determined in bile, and their excretion rate was calculated.

Results of the research and their discussion. In conditions of causing isolated thoracic trauma, abdominal trauma and their combination, a significant disorder of the bile-excreting function of the liver emerges, that is, first of all, manifested by a decrease in the speed of bile excretion and excretion of its main components - cholates and conjugated bilirubin. The severity of liver dysfunction can be divided as follows: isolated thoracic trauma ← isolated abdominal trauma ← combined trauma. In conditions of isolated thoracic trauma, the investigated parameters in comparison with the control reach a minimum level up to the 3d day and normalize to the 7th day. After causing an isolated abdominal trauma the rate of bile excretion and the rate of excretion of total bile acids decrease after a day.

However, all investigated parameters reach a minimum value after 3 days and remain at the same level up to the 7 day. After a combined trauma, the indicators gradually decrease to the 7th day and in each subsequent period become significantly smaller than in the previous one. The use of thiotriazoline for the correction of the detected disorders in the group of animals with combined trauma compared with animals without correction contributed to a significant increase in the rate of bile excretion, excretion of total bile acids and conjugated bilirubin. Although, by the 7th day the indicators did not reach the level of the control group, it can be stated that thiotriazoline exerts a positive effect on the biliary function of the liver.

Conclusions. Combined abdominal and thoracic trauma contributes to a greater reduction in liver functional capacity compared with isolated lesions. They are based on the suppression of the rate of bile excretion and excretion of total bile acids and conjugated bilirubin. The use of thiotriazoline at a dose of 9.1 mg / kg-1 for animals with combined abdominal trauma is accompanied by a significant reduction in biliary function of the liver.


Keywords


combined trauma; thorax; abdomen; biliary excretion; thiotriazoline

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References


N. V. Volotovska, A. A. Hudyma. The role of hemic hypoxia in the pathogenesis of disorders of the biligenic and bile-excreting functions of the liver and associated with skeletal trauma in the early post-traumatic period. Achievements of clinical and experimental medicine. 2011. No. 2 (15). Pp. 31-33.

I. M. Gaida, M. I. Badiuk, Y. I. Sushko. Structure features and course of modern combat trauma of the Armed Forces of Ukraine military men. Pathologia. Vol. 15. No. 1. 2018. Pp. 73-76.

A. A. Hudyma, I. A. Mikhailuk, V. M. Mikhailuk. Dynamics of indices of biligenic function of the liver in response to skeletal, craniocerebral and combined trauma in the early period of a traumatic disease. Medical and clinical chemistry. 2015. T. 17. № 3. Pp. 66-69.

A. A. Hudyma, V. V. Pidgirny. Effects of lansoprazole, metronidazole and clarithromycin on the functional state of the liver and its correction with thiotriazolin in conditions of chronic experimental hepatitis. Zaporozhye medical journal. 2007. № 5. Pp. 33-37.

S. E. Guriev, A. Yu. Fil, O. M. Tanasienko. Analysis of the immediate causes of death in patients with multiple injuries and injuries of the skeleton. Trauma. 2015. Vol.16. №.4. Pp. 7-10.

Preclinical drug research: guidelines; edited by Corresponding Member Academy of Medical Sciences of Ukraine O.V. Stefanov. - K.: Avicenna, 2001. - 528 pp.

O. A. Zachepa, Y. I. Sushko. Antioxidant-prooxidant imbalance in the liver in conditions of combined abdominal and thoracic trauma. Emergency medical care in extreme conditions: All-Ukrainian Research-to-Practice Conference, 22-24 Feb. 2019. Ternopil: Ukrmedkniga, 2019. - Pp. 45-46.

The place of thiotriazoline in the gallery of modern metabolitotropic medicines / I. F. Belenychev, V. A. Vizir, V. I. Mamchur, A. V. Kuryata. Zaporozhye medical journal. 2019. Vol. 21. № 1. Pp. 118-128.

I. A. Mikhailuk, A. A. Hudyma, V. M. Mikhailuk. State of bile-excreting function of the liver in conditions of cranio-skeletal trauma complicated by blood loss. Bulletin of Biological and Medical issues. 2015. № 2. Pp. 182-186.

A. M. Servatovych, T. I. Dzetsiukh. Violations of biochemical composition of bile during the late manifistations of traumatic disease after cranio-skeletal injury and effectiveness of its correction by phytomedications. Medical and clinical chemistry. 2016. Vol. 18, №. 2. Pp. 76-80.

I. S. Chekman, E. A. Posokhova, E. G. Beregovaya. Microsomal enzymatic system of the organism. K., 1996. 80 pp.

Management of the patients with blunt renal trauma: 20 years of clinical experience / L. P. Sarychev, Y. V. Sarychev, H. L. Pustovoyt et al. Wiadomości Lekarskie. 2018. Vol. 71(3 pt. 2). P.719—722.

Prydruha S. M. Pathogenetic features the late current manifestations in an experiment traumatic disease and its correction tiotryazolin. Journal of Health Sciences. 2013. № 3. Р. 111-120.


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