Parodontoprotective action of oral applications of soy isoflavons in rats after orthodontic operation on the background of experimental diabetes mellitus

Mohammed Ali Mehrdad Azari, A. L. Morozov



Background.  To determine the periodontoprotective effect of soy isoflavones in rats after orthodontic surgery on the background of diabetes mellitus.

Methods. In rats, type 1 diabetes was reproduced with aloxane (intraperitoneally at a dose of 100 mg / kg once). From day 12, rats were fitted with orthodontic springs. One of these groups of rats received oral gel applications with soy isoflavones from the first day of the experiment at a dose of 0.5 ml daily for 34 days. The activity of elastase, catalase, urease, lysozyme and the content of malondialdehyde (MDA) were determined in the gum. In the alveolar process of the lower jaw, the activity of alkaline (alkaline phosphatase) and acid (APH) phosphatases, the content of calcium and protein were determined. The antioxidant-prooxidant index (API) was calculated by the ratio of catalase activity and MDA content, the degree of dysbiosis was calculated by the ratio of the relative activities of urease and lysozyme, the mineralizing index (MI) was calculated by the ratio of alkaline phosphatase and APH activity, and the degree of mineralization (DM) was calculated by the ratio of calcium and protein.

Results. In rats with diabetes mellitus, the level of elastase, MDA, urease and the degree of dysbiosis increased with a decrease in the API index and lysozyme activity. Orthodontic surgery showed a certain tendency to normalize these parameters, while the application of a gel containing soy isoflavones completely normalized all indicators. In the bone tissue of periodontium in rats with diabetes mellitus, the activity of alkaline phosphatase and MI decreased, but the activity of APH increased. Orthodontic surgery significantly increased the activity of alkaline phosphatase, slightly changing the activity of APH and the index of API. Application of the gel with isoflavones completely normalized the activity of phosphatases, MI and significantly increased the degree of mineralization of bone tissue.

Conclusion. Rats with type 1 diabetes develop periodontitis. Orthodontic surgery does not exacerbate the pathological condition, showing even a slight tendency to normalize. Oral applications of a gel containing soy isoflavones have a periodontoprotective effect, normalizing all biochemical parameters of the gums and bone periodontal tissue.


periodontal disease; diabetes mellitus; orthodontics; inflammation; dysbiosis; isoflavones; oral gels

Full Text:



Sykes LM, Sukha A. Potential risk of serious oral infections in the diabetic patient: a clinical report. J. Proshet. Dent. 2001; 86: 569-573.

Mutoh T, Honda E, Matsumoto K. [and others]. Study of oral microflora in diabetes mellitus patients. J. Dent. res. 2000; 79(Spec. is.): 2013.

Basov AA, Bykov IM, Melnonyan KI. Changes in immunological reactivity and free radical oxidation processes in the oral fluid in patients with type 2 diabetes mellitus. International Journal of Applied and Fundamental Research. 2014; 2: 31-34.

Fesenko UA, Malygina DA. Influence of diabetes mellitus on clinical and laboratory indices of inflammatory diseases of maxillofacial area in children. Dentist. 2010; 1(139): 32-33.

Ota M, Seshima F, Okubo N. [and others]. A collaborative approach to care for patients with periodontitis and diabetes. Bull. Tokyo Dent. Coll. 2013; 54(1): 51-57.

Demyanenko SA, Morozov AL, Denga AE. Influence of the experimental orthodontic operation on the periodontal condition in rats with metabolic syndrome. Parodontology. 2017; XXIIIb(4(85): 4-7.

Badanov RM. Development of a differentiated approach to the prevention and treatment of prosthetic stomatitis in patients with diabetes mellitus. Odessky medical journal. 2011; 1(123): 36-40.

Azari Mehrdad Mohammad Ali. Periodontal condition in rats with experimental diabetes mellitus after orthodontic surgery. Journal of Education, Health and Sport. 2019; 9(10): 246-252.

Makarenko OA. How to protect bone tissue. Odessa, KP OGT, 2013: 52.

Levitsky AP, Makarenko OA, Selivanskaya IA [and others]. The use of mucosal gels in dentistry. Оdеssа, KP OGT, 2012: 20.

Gorokhovskiy VN, Denga OV, Denga AE [and others]. Modeling of orthodontic movement of teeth in rats. In book. Schneider SA, Levitsky AP "Experimental stomatology". Part 1. Experimental models of dental diseases. Odessa, 2017: 128-132.

Levitsky AP, Denga OV, Makarenko OA [and others]. Biochemical markers of inflammation of oral cavity tissue: method guidelines. Оdеssа, KP OGT, 2010: 16.

Levitsky AP, Makarenko OA, Selivanskaya IA [and others]. Enzymatic method for determining oral dysbiosis for screening pro and prebiotics: guidelines. Kiev, State Pharmacological Center, 2007: 22.

Levitsky AP, Makarenko OA, Denga OV [and others]. Experimental methods of research of stimulants of osteogenesis: methodical recommendations. Kiev, State Pharmacological Center, 2005: 50.

Levitsky AP, Makarenko OA, Khodakov ІV [and others]. The enzymatic method of the estimation of the state of osseous tissue. Odessa Medical Journal. 2006; 3: 17-21.

Lapach SN, Chubenko AV, Babich PN Statistical methods in biomedical research using Excel. Kiev. Morion, 2000: 320.

Levitsky AP, Tsiselsky Yu.V., Dem'yanenko SO [and others]. The use of prebiotics for the prophylaxis of stomatological complications in patients with diabetes mellitus: methodical recommendations. Оdеssа, KP OGT, 2010:18.

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska, EuroPub database, NLM Catalog Result - NCBI, BASE, Russian Sciences Index, Arianta.

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:


PBN Poland



Redaction, Publisher and Editorial Office

Publisher and Editorial Office
Department of Physical Culture,
Faculty of Earth Sciences and Spatial Management,
Nicolaus Copernicus University in Toruń, Poland
Address: Str. Lwowska 1, 87-100 Toruń, Poland

  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48


Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski


Partnerzy platformy czasopism