Efficiency of ademethionine in oxidative stress in tissues of irradiated rats

Abstract

Introduction. Ionizing radiation in low doses of low intensity causes prolonged activation of lipid per oxidation and depletion of the antioxidant system in a living organism. Moreover, Ademethionine is currently being considered as a promising antioxidant.

Method. Experimental studies were carried out on 60 sexually mature male Wistar rats. The animals were irradiated in a total dose of 1Gy on a γ-therapeutic device AGAT-R No. 83 (isotope ⁶⁰Co). At the end of the total dose, the rats were injected intraperitoneally with Heptral (ademethionine) after 15 minutes, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 144, 156 hours after radiation exposure at the rate of 10 mg / kg mass. After the introduction of Heptral, the animals were taken into the experiment after 24 hours, 3, 7, 15 days. In homogenates of the spleen and thymus of animals, the amount of oxidized and reduced forms of pyridine nucleotides was determined.

Results. Chronic γ-irradiation in a total dose of 1Gy leads to a significant decrease in the content of reduced forms of pyridine nucleotides in the spleen and thymus of rats. Administration of Heptral to irradiated animals normalized oxidative homeostasis. So, on the 7th day of the experiment, the amount of oxidized forms of pyridine nucleotides in the spleen was 47.3% lower, and reduced - 36.3% higher than in animals that did not receive treatment. At the end of the observation period, the reduction coefficient of pyridine nucleotides in the spleen slightly differed from the control level. In comparison with irradiated animals, which were not injected with Heptral, the NADP content was lower by 70.3%, and NADPH₂ - higher by 48.8%.

Conclusion. The course administration of Heptral to irradiated animals leads to the normalization of the reduction factor of pyridine nucleotides. According to its mechanism of action, Heptral can be used in the complex treatment of low- intensity radiation injuries in low doses.