MiRNA expression profile in serum of osteoarthritis patients

Dariusz Szala, Joanna Czech, Marzena Skrzypa, Marta Kopanska, Dorota Hanf-Osetek, Krzysztof Gargasz, Grzegorz Guzik, Slawomir Snela, Izabela Zawlik

DOI: http://dx.doi.org/10.12775/JEHS.2019.09.11.001

Abstract


Introduction: Osteoarthritis (OA) is the most common form of arthritis that affects millions of people worldwide. Despite advances in medicine, OA still remains incurable. Scientists are still seeking candidates for biomarker-based risk stratification and the early detection of disease. Measurement of miRNAs in the serum may become a powerful tool in the development of diagnostic biomarker.
Purpose: The aim of this study was to examine the expression profiles of 20 miRNAs in the serum of patients with OA.

Methods: We used RT-qPCR method to measure the expression profile of selected miRNAs.

Results: Six miRNAs showed differential expression between OA and normal serum samples. miR-146a-5p and miR-98-5p were significantly upregulated (P < 0.05), while miR-222-3p, miR-22-3p, miR-27a-3p and miR-93-5p were downregulated (P < 0.05) compared to the control group. To the best of our knowledge, this is the first work that shows that expression of these six miRNAs is different between serum OA samples and healthy controls. However, we did not find any statistically significant relationship between the miRNAs expression and clinical characteristics.

Conclusions: Our findings suggest that miRNAs can be used as potential biomarkers for OA, but further research is needed to assess the usefulness of serum miRNAs in miRNA-based prognostic and therapeutic approaches.

 


Keywords


expression; miRNA; osteoarthritis; serum

Full Text:

PDF

References


Goldring MB, Goldring SR. Osteoarthritis. Journal of cellular physiology 2007;213(3):626-34.

Zhang Y, Jordan JM. Epidemiology of osteoarthritis. Clin Geriatr Med 2010;26(3):355-69.

Felson DT, Zhang Y. An update on the epidemiology of knee and hip osteoarthritis with a view to prevention. Arthritis Rheum 1998;41(8):1343-55.

Lawrence RC, Felson DT, Helmick CG, Arnold LM, Choi H, Deyo RA, et al. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II. Arthritis Rheum 2008;58(1):26-35.

Lau EC, Cooper C, Lam D, Chan VN, Tsang KK, Sham A. Factors associated with osteoarthritis of the hip and knee in Hong Kong Chinese: obesity, joint injury, and occupational activities. Am J Epidemiol 2000;152(9):855-62.

Amoako AO, Pujalte GG. Osteoarthritis in young, active, and athletic individuals. Clinical medicine insights Arthritis and musculoskeletal disorders 2014;7:27-32.

Roos EM. Joint injury causes knee osteoarthritis in young adults. Current opinion in rheumatology 2005;17(2):195-200.

Goldring MB, Otero M. Inflammation in osteoarthritis. Current opinion in rheumatology 2011;23(5):471-8.

Wahid F, Shehzad A, Khan T, Kim YY. MicroRNAs: synthesis, mechanism, function, and recent clinical trials. Biochim Biophys Acta 2010;1803(11):1231-43.

Satoh J, Tabunoki H. Comprehensive analysis of human microRNA target networks. BioData Min 2011;4:17.

Macfarlane LA, Murphy PR. MicroRNA: Biogenesis, Function and Role in Cancer. Curr Genomics 2010;11(7):537-61.

Li M, Marin-Muller C, Bharadwaj U, Chow KH, Yao Q, Chen C. MicroRNAs: control and loss of control in human physiology and disease. World journal of surgery 2009;33(4):667-84.

Diaz-Prado S, Cicione C, Muinos-Lopez E, Hermida-Gomez T, Oreiro N, Fernandez-Lopez C, et al. Characterization of microRNA expression profiles in normal and osteoarthritic human chondrocytes. BMC musculoskeletal disorders 2012;13:144.

Beyer C, Zampetaki A, Lin NY, Kleyer A, Perricone C, Iagnocco A, et al. Signature of circulating microRNAs in osteoarthritis. Annals of the rheumatic diseases 2015;74(3):e18.

Dai L, Zhang X, Hu X, Liu Q, Man Z, Huang H, et al. Silencing of miR-101 Prevents Cartilage Degradation by Regulating Extracellular Matrix-related Genes in a Rat Model of Osteoarthritis. Molecular therapy : the journal of the American Society of Gene Therapy 2015;23(8):1331-40.

Park SJ, Cheon EJ, Lee MH, Kim HA. MicroRNA-127-5p regulates matrix metalloproteinase 13 expression and interleukin-1beta-induced catabolic effects in human chondrocytes. Arthritis and rheumatism 2013;65(12):3141-52.

Li ZC, Han N, Li X, Li G, Liu YZ, Sun GX, et al. Decreased expression of microRNA-130a correlates with TNF-alpha in the development of osteoarthritis. International journal of clinical and experimental pathology 2015;8(3):2555-64.

Seidl CI, Martinez-Sanchez A, Murphy CL. Derepression of MicroRNA-138 Contributes to Loss of the Human Articular Chondrocyte Phenotype. Arthritis Rheumatol 2016;68(2):398-409.

Yamasaki K, Nakasa T, Miyaki S, Ishikawa M, Deie M, Adachi N, et al. Expression of MicroRNA-146a in osteoarthritis cartilage. Arthritis and rheumatism 2009;60(4):1035-41.

Li L, Jia J, Liu X, Yang S, Ye S, Yang W, et al. MicroRNA-16-5p Controls Development of Osteoarthritis by Targeting SMAD3 in Chondrocytes. Current pharmaceutical design 2015;21(35):5160-7.

Ukai T, Sato M, Akutsu H, Umezawa A, Mochida J. MicroRNA-199a-3p, microRNA-193b, and microRNA-320c are correlated to aging and regulate human cartilage metabolism. Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2012;30(12):1915-22.

Zhang D, Cao X, Li J, Zhao G. MiR-210 inhibits NF-kappaB signaling pathway by targeting DR6 in osteoarthritis. Scientific reports 2015;5:12775.

Zhang Y, Jia J, Yang S, Liu X, Ye S, Tian H. MicroRNA-21 controls the development of osteoarthritis by targeting GDF-5 in chondrocytes. Experimental & molecular medicine 2014;46:e79.

Song J, Jin EH, Kim D, Kim KY, Chun CH, Jin EJ. MicroRNA-222 regulates MMP-13 via targeting HDAC-4 during osteoarthritis pathogenesis. BBA clinical 2015;3:79-89.

Iliopoulos D, Malizos KN, Oikonomou P, Tsezou A. Integrative microRNA and proteomic approaches identify novel osteoarthritis genes and their collaborative metabolic and inflammatory networks. PloS one 2008;3(11):e3740.

Wang T, Xu Z. miR-27 promotes osteoblast differentiation by modulating Wnt signaling. Biochemical and biophysical research communications 2010;402(2):186-9.

Akhtar N, Rasheed Z, Ramamurthy S, Anbazhagan AN, Voss FR, Haqqi TM. MicroRNA-27b regulates the expression of matrix metalloproteinase 13 in human osteoarthritis chondrocytes. Arthritis and rheumatism 2010;62(5):1361-71.

Tornero-Esteban P, Rodriguez-Rodriguez L, Abasolo L, Tome M, Lopez-Romero P, Herranz E, et al. Signature of microRNA expression during osteogenic differentiation of bone marrow MSCs reveals a putative role of miR-335-5p in osteoarthritis. BMC musculoskeletal disorders 2015;16:182.

Min Z, Zhang R, Yao J, Jiang C, Guo Y, Cong F, et al. MicroRNAs associated with osteoarthritis differently expressed in bone matrix gelatin (BMG) rat model. International journal of clinical and experimental medicine 2015;8(1):1009-17.

Borgonio Cuadra VM, Gonzalez-Huerta NC, Romero-Cordoba S, Hidalgo-Miranda A, Miranda-Duarte A. Altered expression of circulating microRNA in plasma of patients with primary osteoarthritis and in silico analysis of their pathways. PloS one 2014;9(6):e97690.

Kopańska M, Szala D, Czech J, et al. "MiRNA expression in the cartilage of patients with osteoarthritis." Journal of orthopaedic surgery and research 12.1 (2017): 51.

Paszek S, Gabło N, Barnaś E, et al. "Dysregulation of microRNAs in triple-negative breast cancer." Ginekologia polska 88.10 (2017): 530-536.

Valassi E, Garcia-Giralt N, Malouf J, et al. "Circulating miR-103a-3p and miR-660-5p are associated with bone parameters in patients with controlled acromegaly." Endocrine connections 8.1 (2019): 39-49.

Sharma AR, Jagga S, Lee SS, Nam JS. Interplay between cartilage and subchondral bone contributing to pathogenesis of osteoarthritis. International journal of molecular sciences 2013;14(10):19805-30.

Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, et al. MicroRNA-140 plays dual roles in both cartilage development and homeostasis. Genes & development 2010;24(11):1173-85.

Tardif G, Hum D, Pelletier JP, Duval N, Martel-Pelletier J. Regulation of the IGFBP-5 and MMP-13 genes by the microRNAs miR-140 and miR-27a in human osteoarthritic chondrocytes. BMC musculoskeletal disorders 2009;10:148.

Xu L, Qi X, Duan S, Xie Y, Ren X, Chen G, et al. MicroRNAs: potential biomarkers for disease diagnosis. Bio-medical materials and engineering 2014;24(6):3917-25.

Murata K, Yoshitomi H, Tanida S, Ishikawa M, Nishitani K, Ito H, et al. Plasma and synovial fluid microRNAs as potential biomarkers of rheumatoid arthritis and osteoarthritis. Arthritis research & therapy 2010;12(3):R86.

Yang WH, Tsai CH, Fong YC, Huang YL, Wang SJ, Chang YS, et al. Leptin induces oncostatin M production in osteoblasts by downregulating miR-93 through the Akt signaling pathway. International journal of molecular sciences 2014;15(9):15778-90.

Jones SW, Watkins G, Le Good N, Roberts S, Murphy CL, Brockbank SM, et al. The identification of differentially expressed microRNA in osteoarthritic tissue that modulate the production of TNF-alpha and MMP13. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2009;17(4):464-72.

Okuhara A, Nakasa T, Shibuya H, Niimoto T, Adachi N, Deie M, et al. Changes in microRNA expression in peripheral mononuclear cells according to the progression of osteoarthritis. Modern rheumatology / the Japan Rheumatism Association 2012;22(3):446-57.

Li X, Gibson G, Kim JS, Kroin J, Xu S, van Wijnen AJ, et al. MicroRNA-146a is linked to pain-related pathophysiology of osteoarthritis. Gene 2011;480(1-2):34-41.

Furuzawa-Carballeda J, Macip-Rodriguez PM, Cabral AR. Osteoarthritis and rheumatoid arthritis pannus have similar qualitative metabolic characteristics and pro-inflammatory cytokine response. Clinical and experimental rheumatology 2008;26(4):554-60.

Nakasa T, Nagata Y, Yamasaki K, Ochi M, et al. "A mini-review: microRNA in arthritis." Physiological Genomics 43.10 (2011): 566-570.

Taganov KD, Boldin MP, Chang KJ, Baltimore D. NF-kappa B-depen- dent induction of microRNA miR-146, an inhibitor targeted to signalingproteins of innate immune responses. Proc Natl Acad Sci USA 103:12481–12486, 2006

Li J, Huang J, Dai L, Yu D, Chen Q, Zhang X, et al. miR-146a, an IL-1beta responsive miRNA, induces vascular endothelial growth factor and chondrocyte apoptosis by targeting Smad4. Arthritis research & therapy 2012;14(2):R75.

Watters JW, Cheng C, Pickarski M, Wesolowski GA, Zhuo Y, Hayami T, et al. Inverse relationship between matrix remodeling and lipid metabolism during osteoarthritis progression in the STR/Ort mouse. Arthritis and rheumatism 2007;56(9):2999-3009.

Im HJ, Pacione C, Chubinskaya S, Van Wijnen AJ, Sun Y, Loeser RF. Inhibitory effects of insulin-like growth factor-1 and osteogenic protein-1 on fibronectin fragment- and interleukin-1beta-stimulated matrix metalloproteinase-13 expression in human chondrocytes. The Journal of biological chemistry 2003;278(28):25386-94.

Mitchell PG, Magna HA, Reeves LM, Lopresti-Morrow LL, Yocum SA, Rosner PJ, et al. Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage. The Journal of clinical investigation 1996;97(3):761-8.

Massicotte F, Fernandes JC, Martel-Pelletier J, Pelletier JP, Lajeunesse D. Modulation of insulin-like growth factor 1 levels in human osteoarthritic subchondral bone osteoblasts. Bone 2006;38(3):333-41.

Iwanaga H, Matsumoto T, Enomoto H, Okano K, Hishikawa Y, Shindo H, et al. Enhanced expression of insulin-like growth factor-binding proteins in human osteoarthritic cartilage detected by immunohistochemistry and in situ hybridization. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society 2005;13(5):439-48.

Olney RC, Tsuchiya K, Wilson DM, Mohtai M, Maloney WJ, Schurman DJ, et al. Chondrocytes from osteoarthritic cartilage have increased expression of insulin-like growth factor I (IGF-I) and IGF-binding protein-3 (IGFBP-3) and -5, but not IGF-II or IGFBP-4. The Journal of clinical endocrinology and metabolism 1996;81(3):1096-103.

Dunn W, DuRaine G, Reddi AH. Profiling microRNA expression in bovine articular cartilage and implications for mechanotransduction. Arthritis and rheumatism 2009;60(8):2333-9.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM




Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska, EuroPub database, NLM Catalog Result - NCBI, BASE, Russian Sciences Index, Arianta.

https://zdb-katalog.de/title.xhtml?idn=1080187162

https://europub.co.uk/journals/22625

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Publisher and Editorial Office
Department of Physical Culture,
Faculty of Earth Sciences and Spatial Management,
Nicolaus Copernicus University in Toruń, Poland
Address: Str. Lwowska 1, 87-100 Toruń, Poland

  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

RG Journal Impact: 0.18 *

*This value is calculated using ResearchGate data and is based on average citation counts from work published in this journal. The data used in the calculation may not be exhaustive.

RG Journal impact history

2020Available summer 2021
2018 / 20190.18
20170.07
20160.03

RG Journal impact over time

RG Journal impact
0.180.160.140.120.100.080.060.040.02201620172018
  

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)

Partnerzy platformy czasopism