Structural features of the intervertebral disc in rats
DOI:
https://doi.org/10.12775/JEHS.2021.11.07.006Keywords
intervertebral disc, structural components, norm, ratAbstract
The aim of our work was to study the structural organization of the components of vertebral disc of rats on the microscopic level in the normal state.
This goal was achieved by means of microscopic method of visualization of cellular components of the intervertebral disc of rats. Histological preparations were made in accordance with the conventional method of using the dye hematoxylin, eosin and azan according to the method of Heidenhain, PAS reaction according to MacManus and Alcian blue according to Steedman.
As a result of the microstructural study, it was ascertained that the rat’s intervertebral disc normally consists of a gelatinous (pulpal) nucleus and a fibrous ring. It is separated from the vertebral bodies by a thin layer of cartilaginous tissue of the locking plates and hyaline cartilage, which covers the bodies of adjacent vertebrae. The data that rats do not have locking plates can be rarely found in the literature. The vessels verging the intervertebral disc radially -in front, back and from the sides, branch out from the vessels of vertebral periosteum. In the cartilaginous tissue of intervertebral disc, vessels are not visualized.
References
Nanjo Y, Morio Y, Nagashima H, Hagino H, Teshima R. Correlation between bone mineral density and intervertebral disk degeneration in pre-and postmenopausal women. J. Bone Miner. Metab. 2003;21(1):22-7. DOI: 10.1007/s007740300004
Wang JYX, Kwok AWL, Griffith JF, Leung JCS, Ma HT, Ahuja AT, et al. Relationship Between Hip Bone Mineral Density and Lumbar Disc Degeneration: A Study in Elderly Subjects Using an Eight-Level MRI-Based Disc Degeneration Grading System. J Magn Reson Imaging. 2011;33(4):916-20. DOI:10.1002/jmri.22518
Wang YX, Griffith JF, Ma HT, Leung JC, Ma HT, Ahuja AT, et al. Is decreased BMD associated with less severe lumbar disc degeneration? Bone. 2008;43(Suppl 1):35.
Calleja-Agius J, Muscat-Baron Y, Brincat MP. Estrogens and the intervertebral disc. Menopause Int. 2009;15(3):127-30. DOI: 10.1258/ mi.2009.009016
Chatterjee R. The effect of low vitamin D on chronic non-specific low back pain: A systematic review Chatterjee R, Hemmings S, Laupheimer MW. International Musculoskeletal Medicine. 2016; 38( 2): 43-50.
Rude К, Kirchen ME, Gruber HE, Stashy AA, Meger MH. Magnesiumdeficiency induced bone loss in the rat. Miner, Electrolyte Metal. 1998;24 (5): 314-320.
Zhelnin EV, Zvyagintseva TV, Krivoshapka AV. Posttraumatic regeneration of alveolar bone and its relationship with nitric oxide metabolites in glucocorticoid osteoporosis in rats. Successes of modern natural science. 2014; 5 (1): 34-39. [Ukraine]
Mattei TA. Osteoporosis delays intervertebral disc degeneration by increasing intradiscal diffusive transport of nutrients through both mechanical and vascular pathophysiological pathways. Med Hypotheses.2013;80(5):582-6. DOI: 10.1016/j.mehy.2013.01.030
Povoroznyuk VV, Dedukh NV, Grigorieva NV, Gopkalova IV. K., Experimental osteoporosis. 2012; 228. [Ukraine]
Findlay DM, Atkins GJ. Osteoblast-Chondrocyte Interactions in Osteoarthritis Curr. Osteoporos Rep. 2014;12(1):127-34. DOI: 10.1007/ s11914-014-0192-5
Keller TS, Ziv I, Moeljanto E. Interdependence of lumbar disc and subdiscal bone properties: a report of the normal and degenerated spine. J Spinal Disord. 1993;6(2):106-13. DOI: 10.1097/00024720-199304000-00003
European Convention for the protection of vertebrate animals used for research and other scientific purposes. Strasbourg, March 18, 1986 [Internet] http: zakon.rada.gov.ua/cgi-bin/laws/main.cgi?nreg=994_137
Van der Werf M, Lezuo P, Maissen O, van Donkelaar CC, Ito K. Inhibition of vertebral endplate perfusion results in decreased intervertebral disc intranuclear diffusive transport. J. Anat. 2007;211(6):769-774. DOI: 10.1111/j.1469-7580.2007.00816.x
Simpson EK, Parkinson IH, Manthey B, Fazzalari NL. Intervertebral Disc Disorganization Is Related to Trabecular Bone Architecture in the Lumbar Spine. J Bone Miner Res. 2001;(16):681-87. DOI: 10.1359/jbmr.2001.16.4.681
Osteoporosis: epidemiology, clinic, diagnosis, prevention and treatment: monograph. under ed. Korzha NA, Povoroznyuk VV, Dedukh NV. Kharkiv: Golden Pages, 2002: 648. [Ukraine]
Romeis B. Microscopic technique. M .: Medicine, 1953: 71-72. [Russian]
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