Role of the lipid peroxidation in immunomodulating effects of the nitrogenous metabolites in rats

Abstract

Background. We have previously shown that nitrogenous metabolites have immunomodulatory effects, but the question of mediators of the immunomodulation remains open. We hypothesized the mediating role of mediators of the autonomic nervous system and adaptation hormones as well as reactive oxygen species. Mediating role in the immunomodulation of neuroendocrine factors is analyzed in a previous article. The aim of this study is to analyze the relationships between the parameters of nitrogenous metabolites and lipid peroxidation as well as between latter and immune parameters subordinate modulations by nitrogenous metabolites. Material and methods. Experiment was performed on 60 healthy female Wistar rats. The plasma level and urinary excretion of the nitrogenous metabolites as well as parameters of lipid peroxidation (diene conjugates, malonic dyaldehid, superoxide dismutase, catalase) and neuroendocrine-immune complex were determined. Results. According to the canonical correlation analysis, the constellation of nitrogenous metabolites determines the state of lipid peroxidation by 38,8%. The latter, in turn, determines the constellation of immune parameters (subject to modulation by nitrogenous metabolites) by 61,4%. On the other hand, the coefficient of determination between nitrogenous metabolites and neuroendocrine parameters is 71,5%, and between the latter and immune status – 89,6%. Taken together, neuroendocrine parameters and lipid peroxidation parameters determine the pool of immune parameters subject to modulation by nitrogenous metabolites by 96,7%. It was previously shown that the coefficient of determination between nitrogenous metabolites and a number of immune parameters is 95,8%. Conclusion. The obtained results, taken together with the previous ones, prove that uric acid, bilirubin, urea and creatinine realize their immunomodulatory effects both directly through receptors of immunocytes (aryl hydrocarbon, adenosine and TL) and with the participation of mediators of autonomic nervous and endocrine systems and lipid peroxidation.