Skip to main content Skip to main navigation menu Skip to site footer
  • Register
  • Login
  • Menu
  • Home
  • Current
  • Archives
  • Announcements
  • About
    • About the Journal
    • Submissions
    • Editorial Team
    • Privacy Statement
    • Contact
  • Register
  • Login

Journal of Education, Health and Sport

Oxygen-dependent oxidation of proteins in the pathogenesis of spleen injury caused by acute blood loss, complicated by ischema-reperfusion of the limb and the efficiency of their correction by carbacetam
  • Home
  • /
  • Oxygen-dependent oxidation of proteins in the pathogenesis of spleen injury caused by acute blood loss, complicated by ischema-reperfusion of the limb and the efficiency of their correction by carbacetam
  1. Home /
  2. Archives /
  3. Vol. 10 No. 12 (2020) /
  4. Research Articles

Oxygen-dependent oxidation of proteins in the pathogenesis of spleen injury caused by acute blood loss, complicated by ischema-reperfusion of the limb and the efficiency of their correction by carbacetam

Authors

  • I. V. Iavorska I. Horbachevsky Ternopil National Medical University Ministry of Health of Ukraine

DOI:

https://doi.org/10.12775/JEHS.2020.10.12.037

Keywords

acute blood loss, ischemia-reperfusion of the limb, spleen, oxidative modification of proteins, carbacetam

Abstract

Activation of free radical processes is one of the key mechanisms of acute blood loss and ischemic-reperfusion syndrome. The spleen plays an important role in adaptive-compensatory processes during the blood loss. However, the state of oxidative modifications of proteins (OMP) in the spleen has not been studied enough. There are no data on the effectiveness of carbacetam in these conditions, that showed a pronounced protective effect on the enzyme link of the spleen antioxidant protection.

Objective of research: to study the role of oxygen-dependent protein oxidation in the pathogenesis of spleen involvement during and after acute blood loss complicated by limb ischemia-reperfusion, and the effectiveness of its correction with carbacetam.

Materials and methods. The experiments were performed on 108 nonlinear male rats weighing 200–220 g. All experiments were made using thiopental-sodium anesthesia. In separate groups of animals were simulated limb ischemia-reperfusion and acute blood loss as well as these combined injuries. In a separate group, the detected disorders were corrected with carbacetam. After 1 and 2 hours, as well as after 1, 7 and 14 days in the spleen of the experimental animals was determined the content of oxidative modifications of protein (OMP). The spleen was taken for additional investigation and the content of neutral (OMP370) and basic (OMP430) was determined in its homogenate.

Results. Modeling of acute blood loss, complicated by ischemia-reperfusion of the limb, contributed to the increasing of the processes of uncontrolled oxidative modification of proteins in the spleen. The content of OMP370 rised significantly in the groups of animals, in which only ischemia-reperfusion of the limb or acute blood loss were simulated beginning from 2 h of the experiment. The content of OMP430 exceeded the base level in these experimental groups after 1 and 14 days. Violations of the studied indicators reached a maximum after 1 day, decreased to day 14, but did not reach the level of control group. Carbacetam had a protective effect on the intensity of OMP370-430 after 7 and 14 days of its usage, which puts carbacetam in a line of promising agents of complex therapy of spleen disturbances caused by acute blood loss and ischemic-reperfusion syndrome.

Conclusions: Complication of acute blood loss by ischemia-reperfusion of the limb causes intensification of OMP in the spleen, that, compared with the control is an increase in the content of aldehyde and ketone derivatives of neutral and basic nature throughout the whole experiment with its maximal level at day 1. The use of carbacetam significantly reduces the intensity of OMP in the spleen compared to the group of animals that had no additional drug correction.

References

Gaida I.M., Badyuk M.I., Sushko Y.I. Features of the structure and course of modern combat injury in servicemen of the Armed Forces of Ukraine. Pathology. 2018. 15, №. 1. P. 73 —76.

Gorban I.I .Influence of acute blood loss complicated by ischemia-reperfusion of the limb on the antioxidant-prooxidant balance of the liver and its correction by carbacetam. Achievements of clinical and experimental medicine. 2020. № 2. P. 93—100.

Ena L.M., Chayalo P.P., Hristoforova A.M. Mechanisms of action and prospects of application of drugs for metabolic therapy and ischemic heart disease. Ukr. cardiol. magazine. 2006. № 5. P. 100–106.

Lobov V.V., Nacharov Yu. V., Zubakhin A.A. Hematopoietic function of the spleen in the posthemorrhagic period. Journal of the NSU. Series: biology, clinical medicine. 2006. Vol. 4 Issue. 2. P. 78-83.

Maximov R.V., Gudima A.A., Sidorenko V.M. Dynamics of the balance of antioxidant-prooxidant mechanisms in the internal organs under the influence of arterial tourniquet and limb reperfusion. Hospital surgery. Magazine named after L.Ya.Kovalchuk. 2017. № 1 / P 37—44.

Morpho-functional organization of the spleen of laboratory animals (literature review) / O.V. Avilova, O.O. Prykhodko, O.O. Trach, O.S. Yarmolenko, L.V. Boomeister. The world of medicine and biology. 2017. № 1 (59). P. 175-179.

Meshchishen I.F. Method for determining the oxidative modification of plasma proteins (serum). Bukovin. med. bull 1998. Vol. 2, №1. P. 156-158.

Violation of the content of restored glutathione in the lungs of rats on the background of acute blood loss complicated by ischemia reperfusion of the limb, and their correction with carbacetam / O.V. Stakhiv, A.A. Gudyma, I.V. Korda, Yu. V. Uglyar. Medical and clinical chemistry. 2020. Vol. 22. № 3. P. 74-80.

Prokhorov D.V., Pritulo O.A. Medium-mass molecules as a marker of endogenous intoxication in patients with microbial eczema. Dermatovenereol., Cosmetology., Sexopathol. 2001. № 1 (4). P. 95—97.

Shatsky V.V., Hudyma A.A., Fedonyuk L.Ya. Dynamics of antioxidant-prooxidant balance of cortical and cerebral layers of the kidney after acute blood loss complicated by ischemia-reperfusion of the limb, and its correction by carbacetam. Achievements of clinical and experimental medicine. 2019. № 4. P. 144—153.

Iavorska I.V., Hospodarskyy I.Ya. Dynamics of activity of lipid peroxidation processes in the spleen under the influence of acute blood loss complicated by ischemia-reperfusion of the limb, and its correction by carbacetam. Achievements of clinical and experimental medicine. 2020. № 4. P. 178–185.

Aerobic fitness is associated with cardiomyocyte contractile capacity and endothelial function in exercise training and detraining / O.J.Kemi, R.M.Haram, U.Wisloff [et al.] Circulation. 2004. Vol. 109, № 23. P. 2897-2904.

Berlett B. S., Stadtman E. R. Protein Oxidation and Aging, Disease, and Oxidative Stress. TheJournalofBiologicalChemistry. 1997. 272, № 33. P. 20313–20316. http://dx.doi.org/10.1074/ jbc.272.33.20313

Blaisdell F. W. The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: a review. Cardiovascular Surgery. 2002. Vol. 10, № 6. P. 620—630.

New tourniquet device concepts for battlefield hemorrhage control / J. F. Jr. Kragh, C. Murphy, M. A. Dubicket al. U.S. Army Medical Department Journal. 2011. P.38—48.

Tourniquets and occlusion: the pressure of design / P. L. Wall, D. C. Duevel, M. B. Hassan [et al.]. MilMd. 2013.Vol.178 (5) .P. 578-587.

Downloads

  • PDF

Published

2020-12-29

How to Cite

1.
IAVORSKA, I. V. Oxygen-dependent oxidation of proteins in the pathogenesis of spleen injury caused by acute blood loss, complicated by ischema-reperfusion of the limb and the efficiency of their correction by carbacetam. Journal of Education, Health and Sport [online]. 29 December 2020, T. 10, nr 12, s. 372–381. [accessed 29.3.2023]. DOI 10.12775/JEHS.2020.10.12.037.
  • PN-ISO 690 (Polish)
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
Download Citation
  • Endnote/Zotero/Mendeley (RIS)
  • BibTeX

Issue

Vol. 10 No. 12 (2020)

Section

Research Articles

License

The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0

Stats

Number of views and downloads: 173
Number of citations: 0

Search

Search

Browse

  • Browse Author Index
  • Issue archive

User

User

Current Issue

  • Atom logo
  • RSS2 logo
  • RSS1 logo

Information

  • For Readers
  • For Authors
  • For Librarians

Newsletter

Subscribe Unsubscribe

Tags

Search using one of provided tags:

acute blood loss, ischemia-reperfusion of the limb, spleen, oxidative modification of proteins, carbacetam
Up

Akademicka Platforma Czasopism

Najlepsze czasopisma naukowe i akademickie w jednym miejscu

apcz.umk.pl

Partners

  • Akademia Ignatianum w Krakowie
  • Akademickie Towarzystwo Andragogiczne
  • Fundacja Copernicus na rzecz Rozwoju Badań Naukowych
  • Instytut Historii im. Tadeusza Manteuffla Polskiej Akademii Nauk
  • Instytut Kultur Śródziemnomorskich i Orientalnych PAN
  • Karmelitański Instytut Duchowości w Krakowie
  • Państwowa Akademia Nauk Stosowanych w Krośnie
  • Państwowa Akademia Nauk Stosowanych we Włocławku
  • Państwowa Wyższa Szkoła Zawodowa im. Stanisława Pigonia w Krośnie
  • Polskie Towarzystwo Ekonomiczne
  • Polskie Towarzystwo Ludoznawcze
  • Towarzystwo Miłośników Torunia
  • Towarzystwo Naukowe w Toruniu
  • Uniwersytet im. Adama Mickiewicza w Poznaniu
  • Uniwersytet Mikołaja Kopernika
  • Uniwersytet w Białymstoku
  • Uniwersytet Warszawski
  • Wojewódzka Biblioteka Publiczna - Książnica Kopernikańska
  • Wyższe Seminarium Duchowne w Pelplinie / Wydawnictwo Diecezjalne „Bernardinum" w Pelplinie

© 2021- Nicolaus Copernicus University Accessibility statement Shop