The study of the prooxidant-antioxidant equilibrium in the kidney tissue and blood on the background of ceruloplasmin administration under the conditions of acute kidney injury

Abstract

Acute kidney injury (AKI) remains a relevant medical problem. The promising direction of the AKI treatment is the use of the antioxidants that inhibit free radical processes and prevent the destruction of cell membranes within pathological processes. Ceruloplasmin (CP) is the main antioxidant of blood plasma, that demonstrates membrane protecting effects and antitoxic activity.

The aim of the study was to establish the influence of a single-dose administration of ceruloplasmin on the processes of the peroxidation in the kidney tissue and blood under the conditions of acute kidney injury.

Materials and methods. The influence of ceruloplasmin on the prooxidant-antioxidant equilibrium in the kidney tissue and blood was studied in an experiment on white non-line rats under the conditions of rhabdomyolysis-induced acute kidney injury. The acute kidney injury was modeled by intramuscular administration of 50% glycerol solution at a dose of 8 mg/kg. Ceruloplasmin was administered intraperitoneally at a dose of 7 mg/kg/day. The level of main indicators of prooxidant-antioxidant balance in blood and kidney tissue was determined in 24 h after glycerol administration for induction of acute kidney injury.

Research results and their discussion. There was a deep disruption of the prooxidant-antioxidant equilibrium as a result of the acute kidney injury. AKI causes an activation of the prooxidant processes by increasing the level of the malonic dialdehyde and the oxidized proteins in the kidney tissue and blood. AKI also reduces the antioxidant protection by decreasing the activity of the antioxidant enzymes in the kidneys and blood plasma.

A single-dose administration of ceruloplasmin improved antioxidant protection and reduced peroxidation of lipids and proteins. There was a decrease in levels of malondialdehyde and oxidative modification of proteins. At the same time, the antioxidant protection intensified due to an increase in the activity of glutathione peroxidase and catalase in the kidneys and blood plasma.

Conclusions. A single administration of ceruloplasmin influences prooxidant-antioxidant balance, reducing the content of lipid and protein peroxidation products and increasing the activity of antioxidant enzymes.