Renoprotective action of mucosa-adhesive phytogel "Biotrit" in rats, treated with oral application peroxide sunflower oil
Keywordskidney, nephropathy, oral cavity, peroxide oil, phytogel, bioflavonoids
Aim. Determine the renoprotective activity of oral “Biotrit” gel when exposed to peroxide sunflower oil (PSO).
Methods. PSO was obtained by heating the oil at +180 ° C for 60 minutes in the presence of 1.5% H2O2. Used mucosa-adhesive phytogel "Biotrit", containing bioflavonoids from wheat leaves. Rats received oral applications of PSO at a dose of 0.5 ml/rat for 5 days. A part of the rats received 30 minutes before the application of the PSO application of the “Biotrit” gel. The activity of elastase, catalase, urease, lysozyme and the content of MDA were determined in the kidneys. The ratio of catalase and MDA was calculated the API index, and the ratio of the relative activities of urease and lysozyme – the degree of dysbiosis.
Results. In rats treated with PSO, the activity of elastase, urease, and MDA content increased but lysozyme activity and the API index decreased in the kidneys. Applications of the gel "Biotrit" reduce the activity of elastase, urease, increase the activity of lysozyme and the API index.
Conclusion. Consumption of PSO causes the development of nephropathy; Oral applications of the gel "Biotrit" prevent the development of peroxide nephropathy.
Khajuria A. Lipid peroxidation. Everymanʼs Sci. 1997; 32(3): 109-113.
Wolter R, Jean Cl. Rancissement et antioxydation des lipids en nutrition. Neuropeptides. 1998; 32(1): 203-210.
Sarkisian VA, Kochetkova AA, Bessonov VV [and others]. Toxicological characteristics of the main products of lipid oxidation. Nutrition issues. 2016; 85(6): 80-85. (in Russian)
Voskresenskiy ON, Levitsky AP. Peroxide lipids in the living organism. Questions of medical chemistry 1970; 16(6): 561-581. (in Russian)
Plavinskii SL, Plavinskaia SI. Increased levels of lipid peroxidation products as a risk factor for death in a prospective study. Human physiology. 2002; 28(1): 116-120. (in Russian)
Markov AV. Effect of over-oxidized sunflower oil on the condition of periodontium of rats. Dentistry Bulletin. 2018; 2(103): 14-17. (in Ukrainian)
Labush Iu. Z. The development of stomatitis in rats that consumed рeroxidе sunflower oil. Dentistry Bulletin. 2018; 2: 17-20. (in Ukrainian)
Levitsky AP, Makarenko OA, Selivanskaуa IA [and others]. The experimental prophylaxis of the peroxide periodontitis by antidysbiotic means. Journal of Education, Health and Sport. 2017; 7(2): 682-693.
Markov AV, Labush IuZ, Zubachik VМ [and others]. Therapeutic and preventive action flavan-containing antidisbiotic means on a mucose membrane of the oral cavity of the rats, received peroxide sunflower oil. Phytotherapy. 2018; 2: 33-35. (in Ukrainian)
Volik NA, Beloklitskaya GF. The new adaptogen “Biotrit” in the complex treatment of the periodontal diseases. Dentistry Bulletin. 2000; 5: 28-30. (in Russian)
Demyanenko SA. Mucoprotective and hepatoprotective properties of wheat germ. Dentistry Bulletin. 2008; 5-6: 10-14. (in Russian)
Levitsky AP, Manik IG, Demyanenko SA [and others]. The antiviral and immunemodelling characteristics of the preparation of germinating wheat seads. Phytotherapy. 2009; 3: 43-46. (in Russian)
Demianenko SA, Levitsky AP, Makarenko OA. The effect of Biotrit on the condition of the oral mucosa of rats with toxic hepatitis on the background of dysbiosis. Dentistry Bulletin. 2010; 1: 4-7. (in Russian)
Levitsky AP, Makarenko OA, Denga O. V. [and others]. Phytoadaptogens in the prevention and treatment of dental caries. Odessa, KP OGT, 2013: 119. (in Russian)
Levitsky A. P., Makarenko O. A., Selivanskaya I. A. [and others]. The use of mucosal gels in dentistry: method guidelines.].Оdеssа, KP OGT, 2012: 20. (in Russian)
The "Biotrit" phytogel RC U 20.4-13903778-032/4:2014 for ТU U 20.4-13903778-032:2012. Hygienic conclusion of the State Service of Ukraine for food safety and Consumer Protection № 05.03.02-07/43417; 03.07.2014. (in Russian)
Levitsky AP, Denga OV, Makarenko OA [and others]. Biochemical markers of inflammation of oral cavity tissue: method guidelines. Оdеssа, KP OGT, 2010: 16. (in Russian)
Levitsky AP, Stefanov AV. The methods of the determination of the activity of elastase and its inhibitors: method guidelines. Kiev, GFK, 2002:15. (in Russian)
Levitsky AP, Makarenko OA, Demyanenko SA. Methods of experimental dentistry (teaching aid). Simferopol, Tarpan, 2018: 78. (in Russian)
Girin SV. The modification of the method of the determination of catalase activity in biological substrates. Laboratory diagnosis. 1999; 4:45-46. (in Russian)
Gavrikova LM, Segen IT. Urease activity of oral liquid in patients with acute odontogenic infection of maxillo-facial part. Stomatology. 1996; The extra issue :49-50. (in Russian)
Levitsky AP. Lysozyme instead of antibiotics. Оdеssа, KP OGT, 2005: 74. (in Russian)
Levitsky AP, Denga OV, Selivanskaya IA [and others]. The method of estimation of the degree of dysbiosis (dysbacteriosis) of organs and tissues. Patent of Ukraine 43140. IPC (2009) G01N 33/48. Application number u 200815092. Date of filling: 26.12.2008. Publ.: 10.08.2009. Bul. № 15. (in Ukrainian)
Gozhenko A. I., Levitsky A. P., Stepan V. T., Pustovoit I. P., Badiuk N. S., Maslyukov A. K. Advantages of high olein sunflower oil over palm oil according to biochemical research results - PhOL – PharmacologyOnLine - N 2. - P. 293-301. https://pharmacologyonline.silae.it/files/archives/2020/vol2/PhOL_2020_2_A028_Gozhenko.pdf
Eggum B. Methods to evaluate utilization of proteins by animal. Moscow: Kolos, 1977: 189. (in Russian)
Levitsky AP, Makarenko OA, Khodakov IV. Methods to investigate fats and oils. Odessa, KP OGT, 2015: 32. (in Russian)
Truhacheva NV. Mathematical Statistics in biomedical research using application package Statistica. Moskow, GJeOTAR-Media, 2012: 379. (in Russian)
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