Features of morpho functional changes in periodontal structures in rats with the presence of a photo of a polymer or cement seal
DOI:
https://doi.org/10.12775/JEHS.2020.10.10.029Keywords
photopolymeric filling, cement filling, carbohydrate-containing biopolymer, morphorfunctional state of periodontiumAbstract
High prevalence of caries is a great public health burden. To choose the optimal filling material for the restoration of hard tissues of teeth in caries patients is quite relevant. When choosing materials for dental restoration the doctor is guided by aesthetics quality, long-term guarantee of therapy, comfort at work and cost/quality ratio. The objective: under the conditions of an experiment to establish the relationship between the morphorfunctional state of periodontal components in the presence of either photopolymeric or cement filling and compare the data obtained with the results of intact animals. Materials and methods. The jaws of 41 white laboratory rats were used. 3 groups were formed: group 1 - intact; group 2 included animals with a photopolymer filling; group 3 consisted of rats with a cement filling. Histobacteriological, immunohistochemical, histochemical and statistical methods were used. Results: the animals with photopolymer filling material, in contrast to animals of the intact group, and those with cement filling, had a greater number of microbial cells in the biofilm during the whole experiment. The microbial cells exhibited enzymatic activity, against which there was a reduced number of glycosaminoglycans in the intercellular substance of the epidermis. Conclusions: Against the background of microorganisms increase in the biofilm of the epithelium, changes in carbohydrate-containing biopolymers of the biofilm, reducing the total number of glycosaminoglycans in animals with photopolymer filling material, the number of lymphocytes and functional activity of antigen-presenting cells in the lamina propria of the gums took place. This indicates the activation of humoral nonspecific and specific links of immunity.
References
Blashkova S. L. The role of endogenous antimicrobial peptides in the development of inflammatory periodontal diseases in patients undergoing orthodontic treatment / S. L. Blashkova, I. G. Mustafin G. R. Khaliullina // Fundamental research. - 2014. –No. 4 (part 3) - P. 461-465 (in Russian).
Butvilovskiy A. V. Possibilities of using ketac universal glass ionomer cement in dental practice / A. V. Butvilovskiy // International reviews: clinical practice and health. - 2019. - No. 1. - P. 72–77 (in Russian).
Bykova N. I. Indicators of cellular and humoral immunity in experimental inflammation of periodontal tissues / NI Bykova, AV Odolskiy, VA Grigoryan. // Kuban Scientific Medical Bulletin. - 2016. - No. 6. - P. 20–26 (in Russian).
Domenyuk D. A. The effectiveness of antiseptics on microorganisms associated on the surface of intact and prepared teeth / Domenyuk D. A., S. N. Garage, A.N. Brazhnikova // Materials of the XLIII scientific-practical conference of dentists of the Stavropol Territory "Topical issues of clinical dentistry." - Stavropol, 2010 - P. 252-255 (in Russian).
Dubova L. B. Dental care for patients with aggravated allergic history: textbook / L.V. Dubova, I. Yu. Lebedenko, T. P. Vavilova, I. G. Ostrovskaya / M., 2012. - 79 p. (in Russian)
Zorina O. A. Microbiocenosis of the oral cavity in norm and in inflammatory periodontal diseases / O. A. Zorina, A. A. Kulakov, A.I. Grudyanov // Dentistry - 2011. - No. 1. - P. 73-78 (in Russian).
Kalichkina E. L. Changes in the bacterial structure of the periodontium and its morpho-functional state during the development of the inflammatory process / EL Kalichkina. // Fundamental and clinical medicine. - Vol.2. - 2017. - No. 1. - P. 23–27(in Russian).
Clemin V. A. Diagnosis of allergic status in dentistry (review) / V. A. Clemin, A. A. Vorozhko. // Collection of articles, T. 1. - 2013. - No. 17. - P. 149-157.
Korenevskaya N. А. Biocompatibility of composite filling materials / N.А. Korenevskaya // Bulletin of the Vitebsk State Medical University. - 2016. - Volume 15. - No. 3. - P. 7-17 (in Russian).
Kulakov A. A. The role of protective factors of the body in the pathogenesis of inflammatory periodontal diseases / A. A. Kulakov, O. A., Zorina, O. A. Boriskina // Dentistry. - 2010. - No. 6. - P. 72-77 (in Russian).
Kushch O. G. Adaptive method of setting filling material in the experiment // O.G. Kushch, O.A. Varakuta / Ukrainian Medical Almanac. - 2012. - T. 15.- No. 5 (supplement). - P. 158-160 (in Russian).
Lebedev K. A. Intolerance of denture materials / K. A. Lebedev, A. V. Mitronin, I. D. Ponyakina., 2010. - 208 p. (in Russian).
Ovcharenko LS Immune system of mucous membranes and associated lymphoid tissue: mechanisms of interaction in health and disease, ways of correction / JI.C. Ovcharenko, A.A. Vertegel // Health of Ukraine. —2008. - No. 4. - P. 76−81(in Russian).
Peculiarities of morphological organization of periodontal tissues of schur in the norm // A.B. Fik, Є.A. Paltov, R.I. Onisko [et al.] / Experimental and Clinical Physiology and Biochimia. - 2014. - No. 4. - P. 34-38 (in Russian).
Patent for a utility model № 89088. Method of differentiated detection of B1-lymphocytes in histological specimens / OG Kushch, M.A. Voloshin, О.А. Varakuta. Patent owner Zaporizhia State Medical University. - № 2006 07176; declared 27.06. 2014; publ. 12.12.2014, Bull. № 12 (in Ukr).
Ponedelkina I. Yu. Natural glycosaminoglycans and their chemical modification / I. Yu. Ponedelkina, V. N. Odinokov. // Bulletin of the Academy of Sciences of the Republic of Belarus. - 2009. - No. 1. - P. 26–31 (in Russian)
Ryabokon E.N. The state of local immunity of the oral cavity and autonomic dysfunction of the body in diseases of periodontal tissues / E.N. Ryabokon, A.I. Kryuchko, L.P., Abramova // Ukrainian Stomatological Almanac - 2016. - No. 6. - P. 36-38 (in Russian).
Savichuk N. O. Colonization resistance of the mucous membrane of the empty mouth / N.O. Savichuk / Modern dentistry - 2011. - No. 2 (56) - P. 66-72 (in Russian).
Sergeev Yu. V. Allergy to materials used in orthopedic dentistry / Yu. V. Sergeev, TP Guseva // Dentistry. 2005. - No. 6.-P. 68-73 (in Russian).
Fedoseeva V. N. Pseudoallergic states / V. N. Fedoseeva, A. K. Makovetskaya, O. V. Mislavsky. // Hygiene and sanitation. - 2013. - No. 2. - P. 16-17 (in Russian).
Chebotar I. The Mechanisms of antibiofilm immunity / I. V. Chebotar // Bulletin of the Russian Academy of Medical Sciences-2012.- № 12. - P. 143-150 (in Russian).
Ahmadkhaniha R, Mansouri M, Yunesian M, et al. Association of urinary bisphenol a concentration with type-2 diabetes mellitus [Electronic resource]. Mode of access: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995610/ – Date of access: 10.12.2017.
Dongari-Bagtzoglou A. Pathogenesis of mucosal biofilm infections:challenges and progress // А. Dongari-Bagtzoglou / Exp. Rev. Anti. Infect. Ther.- 2008.- № 6 (2). – Р. 201–208.
Experimental Animal Models in Periodontology: A Review / X.Struillou, H. Boutigny, A. Soueidan, P. Layrolle. // Open Dent J. – 2010. – №4. – Р. 37–47.
Guvva S. Rat as laboratory animal model in periodontology / S. Guvva, M. В. Patil, D. S. Mehta. // Int J Oral Health Sci. – 2017. – №2. – Р. 68–75.
Lee MR, Kim JH, Choi YH, et al. Association of bisphenol A exposure with overweight in the elderly: a panel study [Electronic resource]. Mode of access: https://www.ncbi.nlm.nih.gov/pubmed/25874422 – Date of access: 19.12.2017
Lund F. E., Randall T. D. Effector and regulatory B cells: modulators of CD4(+) T cell immunity. Nat. Rev. Immunol. 2010. Vol. 10, N 4. P. 236-247.
Perez-Lobato R, Mustieles V, Calvente I, et al. Exposure to bisphenol A and behavior in school-age children [Electronic resource]. Mode of access: https://www.ncbi.nlm.nih.gov/pubmed/26654821 – Date of access: 25.12.2017
Rajasarkka J. Monitoring bisphenol A and estrogenic chemicals in thermal paper with yeast-based bioreporter assay / Rajasarkka J, Koponen J., Airaksinen R., Kiviranta H., Virta M. // Anal. and Bioanal. Chem. – 2014. P. 1-10.
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