Research of stimulating efficiency of blood allogeneic plasma fractions on cell proliferation in vitro conditions
DOI:
https://doi.org/10.12775/JEHS.2020.10.08.068Keywords
platelet rich plasma, platelet poor plasma, mesenchymal stem cells, in vitroAbstract
We conducted a comparative experiment on the effect of platelet rich plasma and platelet poor plasma of allogeneic blood of rats on the proliferative activity of different rat cell lines in experimental conditions in vitro. Rat cells such as umbilical cord mesenchymal stem cells, dermal fibroblasts, and fetal myogenic cells were used in the experiment. To compare the proliferative activity of cell lines, the density of the cell population - confluent - was estimated as the percentage of filling the bottom area of the culture vessel with attached and spread cells. As a result of the research it was found that plasma enriched with platelets in the form of 5% supplement to the growth medium has a pronounced stimulating effect on the proliferative activity of fibroblasts, but less effective in the case of cell culture of mesenchymal stem cells. Platelet poor plasma has a physiological effect on the cell cultures of mesenchymal stem cells and rat fibroblasts similar to fetal calf serum. Myogenic rat cells have a high proliferative potential and do not require additional stimulation of platelet rich plasma and platelet poor plasma at the concentrations studied by us.
References
Dzhyvak, V., Klishch, I. Efficacy of platelet-rich blood plasma in induction of muscle tissue healing in an experimental study. Hospital surgery. 2020. №3 (91). P. 36-43. DOI: https://doi.org/10.11603/2414-4533.2020.3.11461
Bissell L., Tibrewal S., Sahni V., Khan W. S. Growth factors and platelet rich plasma in anterior cruciate ligament reconstruction // Curr. Stem Cell Res. Ther. — 2014. — 10, №1. — P. 19-25.
The efficacy of platelet-rich plasma in grafted maxil lae. A randomised clinical trial / M. Badr, P. Coulthard, R.Alissa, R. Oliver // European Journal Oral Implantology. – 2010. – Vol. 3. – №3. – P. 233–244.
Achkasov EE, Bezuglov E. N., Ulyanov AA, Kurshev VV Application of platelet-enriched autoplasma in clinical practice // Biomedicine. - 2013. - № 4. - P. 46-59.
Influence of different concentrations of platelets in blood plasma on chondrogenesis of cells of pulpal nucleus of rats in vitro / EG Pedachenko, MV Khyzhnyak, IG Vasilieva [etc.] // Journal of the National Academy of Medical Sciences Of Ukraine: scientific journal of the Presidium of the National Academy of Medical Sciences of Ukraine. - 2018. - Volume 24, N 3/4. - P. 232-238. -ISSN 2413-7944
Ivchenko DV The use of autologous plasma enriched in platelets to optimize the reparative regeneration of bone tissue in osteopenia / DV Ivchenko, VV Miroshnikov // Pathology. - 2018. - Vol. 15, № 2 (43). - P. 248-258.
Platelet-rich plasma injection for chronic Achilles tendinopathy: a randomized controlled trial / R.J. de Vos, A. Weir, H.T. van Schie, et al. // JAMA. – 2010. – Vol. 303. – №2. – P. 144–149.
Senzel L., Gnatenko D. V., Bahou W. F. The platelet proteome // Curr Opin Hematol. — 2009. — 5. — P. 329- 333.
The influence of different concentrations of platelets in blood plasma on the chondrogenesis of cells of the pulpal nucleus of rats in vitro [Text] / EG Pedachenko, MV Khyzhnyak, IG Vasilieva [etc.] // Journal of the National Academy of Medical Sciences Of Ukraine: scientific journal of the Presidium of the National Academy of Medical Sciences of Ukraine. - 2018. - Volume 24, N 3/4. - P. 232-238. -ISSN 2413-7944
Dzhyvak V., Klishch I., Datsko T., Khlibovska O. Influence of PRP on morphological changes in muscle in the early period after traumatic muscle injury in the experiment. Journal of Education, Health and Sport. 2020;10(6):171-178. eISSN 23918306.
Cole B.J. Platelet-rich plasma: where are we now and where are we going? / Cole B.J., Seroyer S.T., Filardo G. // Sports Health. –2010. – Vol. 2. – No 3. – P. 203–210.
Heger JI, Froehlich K, Pastuschek J, Schmidt A, Baer C, Mrowka R, Backsch C,Schleußner E, Markert UR, Schmidt A. Human serum alters cell culture behavior and improves spheroid formation in comparison to fetal bovine serum. Exp Cell Res. 2018 Apr 1;365(1):57-65. doi: 10.1016/j.yexcr.2018.02.017. Epub 2018 Feb 21. PMID: 29476836.
Fishchenko VO The effectiveness of platelet-enriched plasma in limited cartilage damage: a review of basic and clinical studies / VO Fishchenko, OV Fishchenko, MV Rybinsky, S. Yu. Yaremin, VV Hutsol, MM Andrushenko // Bulletin of orthopedics, traumatology and prosthetics. - 2016. - № 3. - P. 63-69.
Masurkewitsch A. J., Kowpak W. W., Danіlow W. B. (2014) Klіtinnі technologії u weterinarnіj medizinі. Nawtschal'nij pocіbnik [Cellular technology in veterinary medicine. Tutorial]. K.: KOMPRINT.
Kovpak VV Proliferative activity of rat mesenchymal stem cells under the influence of culture medium / VV Kovpak, OS Kovpak. // Scientific reports of the National University of Life and Environmental Sciences of Ukraine. - 2016. - № 3.
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official Journal of the European Union, 2010, L276, 33-79.
Guide for the care and use of laboratory animals. 8th ed. Washington: The National Academies Press. (2011).
Mishra A. Buffered platelet-rich plasma enhances mesenchymal stem cell proliferation and chondrogenic differentiation / Mishra A., Tummala P., King A. // Tissue Eng. Part C Methods.– 2009. – Vol. 15. – No 3. – P. 431–435.
Murphy M.B. Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation / Murphy M.B., Blashki D., Buchanan R.M. // Biomaterials. – 2012. – Vol. 33. – No 21. – P. 5308–5316.
Kruger J.P. Human platelet-rich plasma induces chondrogenic differentiation of subchondral progenitor cells in polyglycolic acid-hyaluronan scaffolds / Kruger J.P., Ketzmar A.K., Endres M. // J. Biomed. Mater. Res. B Appl. Biomater. – 2014. – Vol. 102. – No 4. – P. 681–692.
Mifune Y. The effect of platelet-rich plasma on the regenerative therapy of muscle derived stem cells for articular cartilage repair / Mifune Y., Matsumoto T., Takayama K. // Osteoarthritis Cartilage. – 2013. – Vol. 21. – No 1. – P. 175–185.
Xie X. Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration / Xie X., Wang Y., Zhao C. //Biomaterials. – 2012. – Vol. 33. – No 29. – P. 7008–7018.
Feng X. Effect of platelet lysate on chondrogenic differentiation of human umbilical cord derived mesenchymal stem cells in vitro / Feng X., Tian S., Sun K. // Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. – 2011. – Vol. 25. – No 10. – P. 1250–1255.
Zaky S.H. Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering / Zaky S.H., Ottonello A., Strada P. // J. Tissue Eng. Regen. Med. – 2008. – Vol. 2. – No 8. – P. 472–481.
Platelet-rich plasma promotes healing of osteoporotic fractures / L. Chen, X. Yang, G. Huang, et al. // Orthopedics. – 2013. – Vol. 36. – №6. – P. 687–694
Downloads
Published
How to Cite
Issue
Section
License
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 320
Number of citations: 0
