Comparative study of KI-67 and CD44 expression in serrated polyps of the distal colon
DOI:
https://doi.org/10.12775/JEHS.2020.10.07.038Keywords
Intestinal neoplasms, Polyps, Cell Proliferation, CD44 AntigenAbstract
Introduction. Serrated polyps are newly distinguished types of colonic polyps. Nowadays lots of questions about serrated neoplastic pathway are still unclear. The purpose of the study was to compare Ki-67 and CD44 immunohistochemical (IHC) expression levels in different histological types of serrated polyps. Materials and methods. Histopathological and IHC studies of 30 serrated polyps were conducted. IHC study was carried out using antibodies against Ki-67 and CD44. Results of IHC reactions with antibodies against Ki-67 were estimated by immunostained nuclei counting and were expressed in proliferation index (PI) while results of IHC reactions with antibodies against CD44 were estimated by photo digital morphometry and were expressed in immunostained cells relative area (%). Furthermore, distribution of Ki-67+ and CD44+ cells in colonic crypts was examined. Results. It was revealed that hyperplastic polyps (HP) were characterized by medium PI and basal-middle pattern of Ki-67+ cells distribution. HP were also characterized by the median of CD44+ cells area equal to 22,36 (13,15;30,41) % and basal-middle pattern of CD44+ cells distribution. Herewith, direct medium strength correlation between Ki-67 and CD44 expression levels in HP was established. Traditional serrated adenomas (TSA) were characterized by medium PI and diffuse pattern of Ki-67+ cells distribution. TSA were also characterized by the median of CD44+ cells area equal to 25,48 (15,19;29,04) % and upper-middle pattern of CD44+ cells distribution. Direct weak strength correlation between Ki-67 and CD44 expression levels in TSA was established. Sessile serrated adenomas (SSA) were characterized by medium PI and basal pattern of Ki-67+ cells distribution. SSA were also characterized by the median of CD44+ cells area equal to 20,54 (11,25;28,15) % and basal-middle pattern of CD44+ cells distribution. Direct medium strength correlation between Ki-67 and CD44 expression levels in SSA was established.
References
Nagtegaal, I.D., Odze, R.D., Klimstra, D., Paradis, V., Rugge, M., Schirmacher, P., Washington, K.M., Carneiro, F., Cree, I.A. (2020). The 2019 WHO classification of tumours of the digestive system. Histopathology, 76: 182-188. https://doi.org/10.1111/his.13975
Okamoto, K., Kitamura, S., Kimura, T., Nakagawa, T., Sogabe, M., Miyamoto, H., Muguruma, N., Takayama T. (2017). Clinicopathological characteristics of serrated polyps as precursors to colorectal cancer: Current status and management. J Gastroenterol Hepatol.; 32(2): 358-367. https://doi.org/10.1111/jgh.134
Hegazy, A., Daoud, S., A., Ibrahim, W., S., El-Atrebi, K., Saker, M., Abdel-Wahab, N. (2014). Role of Ki-67, P53 and Bcl-2 in Advanced Colorectal Carcinoma (Histopathological and Immunohistochemical Study). Academic Journal of Cancer Research, №7(3), 168-72. https://doi.org/10.5829/idosi.ajcr.2014.7.3.1111
Fujimori, Y., Fujimori, T., Imura, J., Sugai, T., Yao, T., Wada, R., Ajioka, Y., Ohkura, Y. (2012). An assessment of the diagnostic criteria for sessile serrated adenoma/polyps: SSA/Ps using image processing software analysis for Ki67 immunohistochemistry. Diagn Pathol. 7:59. https://doi.org/10.1186/1746-1596-7-59
Pap, Z., Ilues, I., Mocan, S., Denes, L., Nagy-Bota, M., Pavai, Z., Szanto, A. (2015). Changes in immunoexpression of p53, Ki-67, Ets-1, APAF-1 and PTEN in serrated and conventional colon adenomas. Romanian Journal of Morphology and Embryology, 56(4), 1389–1396.
Jung, J.H., Kwon, H.J., Kim, T.J., Cho, H.J., Kim, H.K., Cheung, D.Y., Kim, J.I., Kim, J.K. (2013). Immunohistochemical expression of p53, Bcl-2, and Ki-67 proteins in traditional serrated adenomas of colon. Korean J Gastroenterol. 62(6): 336-43. https://doi.org/10.4166/kjg.2013.62.6.336
Del Gobbo, A., Ferrero, S. (2017) Immunohistochemical Markers as Predictors of Histopathologic Response and Prognosis in Rectal Cancer Treated with Preoperative Adjuvant Therapy: State of the Art. Gastroenterology Research and Practice. 2017, 6pp. https://doi.org/10.1155/2017/2808235
Morath, I., Hartmann, T., Orian-Rousseau, V. (2016). CD44: More than a mere stem cell marker. The International Journal of Biochemistry & Cell Biology. 81: 166-173. https://doi.org/10.1016/j.biocel.2016.09.009
Wang, L., Zuo, X., Xie, K., Wei, D. (2018). The Role of CD44 and Cancer Stem Cells. Methods Mol Biol. 1692: 31-42. https://doi.org/10.1007/978-1-4939-7401-6_3
Chai, N., Zhang, W., Wang, Y., Zhou, Z., Zhang, Y., Liu, H., Wan, J., Qin, J., Wang, S., Wang, Y., Pei, X., Wu, B., Linghu, E. (2013) Lgr5 and CD44 expressions in different types of intestinal polyps and colorectal cancer. Nan Fang Yi Ke Da Xue Xue Bao. 2013;33(7):972-976.
Baker, A.M., Cereser, B., Melton, S., Fletcher, A.G., Rodriguez-Justo, M., Tadrous, P.J., Humphries, A., Elia, G., McDonald, S.A., Wright, N.A., Simons, B.D., Jansen, M., Graham, T.A. (2014) Quantification of crypt and stem cell evolution in the normal and neoplastic human colon. Cell Rep. 8(4): 940-947. https://doi.org/10.1016/j.celrep.2014.07.019
Singh, R., Cheng Tao Pu, L.Z., Koay, D., Burt, A. (2016) Sessile serrated adenoma/polyps: Where are we at in 2016? World Journal of Gastroenterology. 22(34): 7754-59. https://doi.org/10.3748/wjg.v22.i34.7754
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