Mechanisms for providing different reactivity to immobilization stress in male and female rats with different resistance to acute hypoxic hypoxia
DOI:
https://doi.org/10.12775/JEHS.2020.10.04.026Keywords
correlation, oxidative stress, immobilizing stress, resistence to hypoxia, sex, ratsAbstract
Attention of scientists of the world are study the features of increased resistance to hypoxia and chronic stress.
The aim of the study was to trace the features of multidirectional correlations in the mechanisms of heart damage in high- and low-resistance to hypoxic hypoxia rats (HR, LR) in different models of immobilization stress.
Material and methods of investigation. The experiments were performed on 144 outbred HR and LR aged 5.5-6 months, dividing into 3 groups – control and 2 with different model of immobilizing stress. Determine correlation between superoxide dismutase activity (SOD), catalase, GSH, glutathione peroxidase (GP), glutathione reductase (GR), conjugates diene and triene (DC, TC), Schiff bases (SB), TBA-active products, nitrite anion, in the blood – peroxidase activity (PAB), ceruloplasmin (CP), circulating immune complexes (CIC), oxidative modification of proteins.
Results. In HR males rats in repeated every 24 hours stress, with increasing oxidative stress, has the positive correlation with the activity of antioxidant enzymes (SOD, catalase, CP), and in LR with the accumulation of CIC increases GSH. At the stress repeated every 72 hours, various mechanisms of adaptation are noted: in HR animals due to activation of catalase and SOD, in LR – mainly due to catalase and CP. In HR rats-female with stress, which is repeated every 24 hours, adaptation occurs due to activation of SOD and PAB, in LR with the accumulation of CIC – due to PAB and GR. When the stress is repeated every 72 hours, catalase and GH are mainly activated in HR female, in LR – SOD, PAB, CP.
Conclusion. The experiments revealed significant correlations that indicate the individual characteristics of the adaptation of rats to immobilization stress, which depend on resistance to hypoxia and sex.
References
Altitude Omics: The Integrative Physiology of Human Acclimatization to Hypobaric Hypoxia and Its Retention upon Reascent / A. W. Subudhi, N. Bourdillon, J. Bucher [et al.] // PLoS One. 2014;9(3):e92191.
Gibson O. R., Taylor L., Watt P. W., Maxwell N. S. Cross-Adaptation: Heat and Cold Adaptation to Improve Physiological and Cellular Responses to Hypoxia // Sports Med. 2017;47(9):1751–1768.
Glutamate receptors in the nucleus tractus solitarius contribute to ventilatory acclimatization to hypoxia in rat / M. E. Pamenter, J. A. Carr, A. Go [et al.] // J. Physiol. 2014;592(Pt 8):1839–1856.
Colleen G. Julian, Lorna G. Moore Human Genetic Adaptation to High Altitude: Evidence from the Andes // Genes (Basel). 2019;10(2):150.
Shimoda L. A. Let's Talk about Sex: A Novel Mechanism by Which Estrogen Receptor β Limits Hypoxia-Inducible Factor Expression in Pulmonary Endothelial Cells // Am. J. Respir. Cell Mol. Biol. 2018;59(1):11-12.
Miller A. J., Cui J., Luck J. C., Sinoway L. I., Muller M. D. Age and sex differences in sympathetic and hemodynamic responses to hypoxia and cold pressor test // Physiol. Rep. 2019;7(2):e13988.
Souza G.M.P.R., Amorim M.R., Moraes D.J.A., Machado B.H. Sex differences in the respiratory-sympathetic coupling in rats exposed to chronic intermittent hypoxia // Respir. Physiol. Neurobiol. 2018;256:109-118.
Berezovskij V.A. Hypoxia and individual peculiarities of reactivity. – K.: Scientific thought, 1978;2-216. [in Russian]
Kulynskyi V.Y. & Olkhovskyi Y.A. Two adaptation strategies in adverse conditions: resistant and tolerant. The role of hormones and receptors. Advances of modern biology. 1992;112;697–711 [in Russian].
Chevary S., Chaba Y., Sokei Y. The role of superoxide dismutase in the oxidative processes of the cell and the method for its determination in biological materials // Laboratory work. 1985;11:678–681 [in Russian].
Koroliuk M. A., Yvanova L. Y., Maiorova Y.H., Tokarev V.E. Method for determination of catalase activity // Laboratory work. 1988;1:16–19 [in Russian].
Moffat J. A., Armstrong P. W., Marks G. S. Investigations into the role of sulfhydryl groups in the mechanism of action of the nitrates // Canadian Journal of Physiology and Pharmacology. 1982;10(60):1261-1266.
Kruhlikova H. O., Shtutman I. M. Glutathionperoxidase and glutathionreductase activity of rats liver after the introduction of selenite of sodium // Ukrainian Biochemical Journal. 1976;2:227–233. [in Ukrainian]
Gavrilov V. В., Mishkorudnaya M. I. Spectrophotometric determination of the content of lipid hydroperoxides in blood plasma // Laboratory work. 1983;3:33–35. [in Russian]
Коrobeinikov E. N. Modification of the determination of lipid peroxidation products in the reaction with thiobarbituric acid // Laboratory work. 1989;7:8–10. [in Russian]
Changes in concentration of NО2¯ in biological fluids in diseases of gastric cancer / О. Ya. Sclyarov, І. P. Fedorovych, V. М. Коrobov [et al.] // Medical chemistry. 2004;3(6):55-57. [in Ukrainian]
Rakytianskyi V. M., Yefimov V. H. Peroxidase and catalase activity of blood in Holstein cattle for the effects of hydrogum and microelements // Scientific Herald of LNUWMBT named after S. Z. Gzhytsky 2010;12:250-255. [in Ukrainian]
Clinical and laboratory diagnostics. Normative directive legal documents. – К. : МVC “Меdinform”, 2003. – 856 с. [in Ukrainian]
Belozerov Е. S., Маkarov Т. А. Precipitation method for the study of immune complexes in patients with viral hepatitis B. // Laboratory work. 1982;12:37-39. [in Russian]
Меshyshen І. F. Method for determination of oxidative modification of blood plasma proteins // Bukovinian Medical Herald. 1998; 1(2):156–158. [in Ukrainian]
Downloads
Published
How to Cite
Issue
Section
License
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 145
Number of citations: 0
