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Journal of Education, Health and Sport

Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid
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  • Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid
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  3. Vol. 9 No. 11 (2019) /
  4. Research Articles

Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid

Authors

  • H. O. Vyshnevska SI “Institute of Dentistry and Maxillofacial Surgery of the National Academy of Medical Sciences of Ukraine”
  • Z. Sh. Kakabadze Tbilisi State Medical University
  • S. A. Schnayder SI “Institute of Dentistry and Maxillofacial Surgery of the National Academy of Medical Sciences of Ukraine”
  • O. I. Tiron Odesa National Medical University
  • O. F. Dzygal Odesa National Medical University

DOI:

https://doi.org/10.12775/JEHS.2019.09.11.038

Keywords

amniotic membrane, growth factors, hyaluronic acid, morphology, scanning electron microscopy

Abstract

Nowadays the development of novel biomaterials which would promote tissue lesions regeneration is one of the crucial tasks of regenerative medicine. In the current research we developed a biological active membrane composed of decellularized, lyophilized human amniotic membrane in combination with growth factors of platelet-rich plasma (PRP) and hyaluronic acid. On the basis of histological examination, scanning electron microscopic examination and DNA content analyses it was drawn a conclusion that Sodium Dodecyl Sulfate (SDS) (Sigma) solution and 1% Triton X-100 solution completely remove all existing cells of placenta and chorionic-amniotic membrane without impairment of their structure. After the processes of decellularization and lyophilization the DNA content was less than 2%. The PRP growth factors as the components biological matrix provide faster regeneration. The latter are fixed on the surface of cellular structure of decellularized, lyophilized human amniotic membrane by the reservoir of gelatinous hyaluronic acid

References

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El Sheshtawy A.S., Nazzal H., El Shahawy O.I., El Baz A.A., Ismail S.M., Kang J., Ezzat K.M. The effect of platelet-rich plasma as a scaffold in regeneration/revitalisation endodontics of immature permanent teeth assessed using 2-dimensional radiographs and Cone Beam Computed Tomography: A randomised controlled trial. Int Endod J. 2020; 6. doi: 10.1111/iej.13303.

De Melo B.A.G., França C.G., Dávila J.L., Batista N.A., Caliari-Oliveira C., d'Ávila M.A., Luzo Â.C.M., Lana J.F.S.D., Santana M.H.A. Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine. Mater Sci Eng C Mater Biol Appl. 2020; 109:110547.

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Al Shehadat S., Gorduysus M.O., Abdul Hamid S.S., Abdullah N.A., Samsudin A.R., Ahmad A. Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary study. Eur J Dent. 2018; 12(4): 574–578.

Onerci M. The effects of lyophilized homograft amniotic membrane on wound healing on rabbits. Acta Otorhinolaryngol Ital. 1991; 11: 491–496.

Barer G.M., Surazhev B.Yu., Yanushevich О.О. Comparative analysis of the use of insulating membranes for targeted regeneration of periodontal tissues // Abstr. of the scientific session of TsNIIS dedicated to the 35th anniversary of the Institute "Science - Practice". Мoscow. 1998: 110-113 [In Russian].

Kakabadze A., Karalashvili L., Makashvili M., Abiatari I., Kakabadze Z. Anatomical Justification for the Use of Human Placenta as a Host for in Vivo Cell and Tissue Transplantation. Asian Journal of Pharmacy, Nursing and Medical Sciences. 2014; 2 (1): 33-37

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Published

2019-11-29

How to Cite

1.
VYSHNEVSKA, H. O., KAKABADZE, Z. Sh., SCHNAYDER, S. A., TIRON, O. I. and DZYGAL, O. F. Development and study of perspective clinical properties of biologically active matrix composed of decellularized lyophilized human amniotic membrane in combination with platelet-derived growth factors and hyaluronic acid. Journal of Education, Health and Sport. Online. 29 November 2019. Vol. 9, no. 11, pp. 410-418. [Accessed 4 July 2025]. DOI 10.12775/JEHS.2019.09.11.038.
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Vol. 9 No. 11 (2019)

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Research Articles

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