Lipopolysaccharide increases lysozyme adhesion to chitin
DOI:
https://doi.org/10.12775/JEHS.2019.09.11.021Keywords
lysozyme, lipopolysaccharide, chitin, bacteriolysis, adsorption of lysozyme on chitinAbstract
Background. To study the effect of lipopolysaccharide on the adsorption of lysozyme on chitin.
Methods. Egg white lysozyme, lipopolysaccharide and chitin were used. The lyophilized cells of M. lyzodeikticus were used as a lysozyme substrate. Lysozyme activity was determined by the bacteriolytic method using different concentrations (8-512 μg/ml). Lysozyme adsorption on chitin was determined by the loss of lysozyme from solution after shaking with chitin. The effect of lipopolysaccharide (0.6-40 μg/ml) on lysozyme adsorption was evaluated by the loss of lysozyme from the solution after shaking with 50 mg of chitin.
Results. In determining the activity of lysozyme by the bacteriolytic method, the linear nature of the dependence of activity on the concentration of the enzyme is observed only at low concentrations (8-100 μg / ml). Lipopolysaccharide does not affect the bacteriolytic activity of lysozyme, however, dose-dependently increases the adsorption of lysozyme on chitin.
Conclusion. Lipopolysaccharide increases the adsorption of lysozyme on chitin.
References
Barabash AD, Levitsky AP. Enzymatic mechanisms of antimicrobe defence of at oral cavity. Questions of medical chemistry. 1978; 24(3): 291-310. (in Russian)
Levitsky AP. Lysozyme instead of antibiotics. Оdеssа, KP OGT, 2005: 74. (in Russian)
Sariri R, Gafuri Kh. Proteins of tears: normal, with eye disease and when wearing contact lenses. Biochemistry. 2008; 73(4): 469-482. (in Russian)
Bukharin OV, Usvetsov BYa, Matiiash IN [and others]. The reference of lysozyme antimicrobe activity. Antibiotics. 1976; 9: 805-808. (in Russian)
Dobrotina NA, Kazatskaia ZhA, Emelianova TIu. Lysozyme as immunological modulator. Questions of medical chemistry. 1988; 33: 66-69. (in Russian)
Maianskii DN, Kutina SN, Shcherbakova EG. Influence of lysozyme on hepatocytes resistence. Antibiotics and chemotherapy. 1988; 33(2): 128-131. (in Russian)
Kutina SN, Zubakhin AA. Liver resistance to damage of ССI4 at stimulation of macrophages with the preparations of different categories. BEBIM. 2000; 129(6): 620-622. (in Russian)
Levashov PA, Matolygina DA, Ovchinnikova ED [and others]. New sorbent based on covalently immobilized lysozyme to remove bacterial lipopolysaccharide (endotoxin) from biological fluids. Biochemistry. 2019; 84(1): 100-108. (in Russian)
Yakovlev MYu. The elements of endotoxin theory of human physiology and pathology. Physiology of man. 2003; 29(4): 98-109. (in Russian)
Levitsky AP, Ostafiichuk MA, Uspenskii OE [and others]. The influence of different pathogens on the lysozyme activity into tissues of rat oral cavity. Journal of Education, Health and Sport. 2017; 7(8): 1070-1081.
Cherkasov IA, Kravchenko NA. An improved method for the isolation of lysozymes by enzyme-substrate chromatography. Biochemistry. 1969; 34(5): 1089-1091. (in Russian)
Cherkasov IA, Kravchenko NA. Copyright certificate 178771 USSR, IPC C12K. A method for producing enzyme preparations of lysozymes from their raw materials. Application number 943666/28-13. Date of filling: 17.11.1965. Publ.: 19.11.1966. Bul. № 4. (in Russian)
Lakhtin V, Kostanova E, Arbatskii N. Purification and characterization of multiple forms of papaya latex lysozyme. Applied biochemistry and microbiology. 1995; 2: 247-254. (in Russian)
Islam R, Kite J, Baker AS [and others]. Affinity purification of hen egg lysozyme using sephadex G75. African J. of Biotechnology. 2006; 5(20): 1902-1908.
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