Humanities
Skip to main content Skip to main navigation menu Skip to site footer
  • Register
  • Login
  • Menu
  • Home
  • Current
  • Archives
  • Announcements
  • About
    • About the Journal
    • Submissions
    • Editorial Team
    • Privacy Statement
    • Contact
  • Register
  • Login

Journal of Education, Health and Sport

Lipopolysaccharide increases lysozyme adhesion to chitin
  • Home
  • /
  • Lipopolysaccharide increases lysozyme adhesion to chitin
  1. Home /
  2. Archives /
  3. Vol. 9 No. 11 (2019) /
  4. Research Articles

Lipopolysaccharide increases lysozyme adhesion to chitin

Authors

  • A. P. Levitsky State Etablishment «The Institute of Stomatology and Maxillo-Facial Surgery of the National Academy of Medical Science of Ukraine»
  • L. M. Mudrik State Etablishment «The Institute of Stomatology and Maxillo-Facial Surgery of the National Academy of Medical Science of Ukraine»
  • L. N. Khromagina State Etablishment «The Institute of Stomatology and Maxillo-Facial Surgery of the National Academy of Medical Science of Ukraine»
  • T. A. Sevostianova State Etablishment «The Institute of Stomatology and Maxillo-Facial Surgery of the National Academy of Medical Science of Ukraine»
  • I. A. Selivanskaia State Etablishment «The Institute of Stomatology and Maxillo-Facial Surgery of the National Academy of Medical Science of Ukraine»

DOI:

https://doi.org/10.12775/JEHS.2019.09.11.021

Keywords

lysozyme, lipopolysaccharide, chitin, bacteriolysis, adsorption of lysozyme on chitin

Abstract

Background. To study the effect of lipopolysaccharide on the adsorption of lysozyme on chitin.

Methods. Egg white lysozyme, lipopolysaccharide and chitin were used. The lyophilized cells of M. lyzodeikticus were used as a lysozyme substrate. Lysozyme activity was determined by the bacteriolytic method using different concentrations (8-512 μg/ml). Lysozyme adsorption on chitin was determined by the loss of lysozyme from solution after shaking with chitin. The effect of lipopolysaccharide (0.6-40 μg/ml) on lysozyme adsorption was evaluated by the loss of lysozyme from the solution after shaking with 50 mg of chitin.

Results. In determining the activity of lysozyme by the bacteriolytic method, the linear nature of the dependence of activity on the concentration of the enzyme is observed only at low concentrations (8-100 μg / ml). Lipopolysaccharide does not affect the bacteriolytic activity of lysozyme, however, dose-dependently increases the adsorption of lysozyme on chitin.

Conclusion. Lipopolysaccharide increases the adsorption of lysozyme on chitin.

References

Barabash AD, Levitsky AP. Enzymatic mechanisms of antimicrobe defence of at oral cavity. Questions of medical chemistry. 1978; 24(3): 291-310. (in Russian)

Levitsky AP. Lysozyme instead of antibiotics. Оdеssа, KP OGT, 2005: 74. (in Russian)

Sariri R, Gafuri Kh. Proteins of tears: normal, with eye disease and when wearing contact lenses. Biochemistry. 2008; 73(4): 469-482. (in Russian)

Bukharin OV, Usvetsov BYa, Matiiash IN [and others]. The reference of lysozyme antimicrobe activity. Antibiotics. 1976; 9: 805-808. (in Russian)

Dobrotina NA, Kazatskaia ZhA, Emelianova TIu. Lysozyme as immunological modulator. Questions of medical chemistry. 1988; 33: 66-69. (in Russian)

Maianskii DN, Kutina SN, Shcherbakova EG. Influence of lysozyme on hepatocytes resistence. Antibiotics and chemotherapy. 1988; 33(2): 128-131. (in Russian)

Kutina SN, Zubakhin AA. Liver resistance to damage of ССI4 at stimulation of macrophages with the preparations of different categories. BEBIM. 2000; 129(6): 620-622. (in Russian)

Levashov PA, Matolygina DA, Ovchinnikova ED [and others]. New sorbent based on covalently immobilized lysozyme to remove bacterial lipopolysaccharide (endotoxin) from biological fluids. Biochemistry. 2019; 84(1): 100-108. (in Russian)

Yakovlev MYu. The elements of endotoxin theory of human physiology and pathology. Physiology of man. 2003; 29(4): 98-109. (in Russian)

Levitsky AP, Ostafiichuk MA, Uspenskii OE [and others]. The influence of different pathogens on the lysozyme activity into tissues of rat oral cavity. Journal of Education, Health and Sport. 2017; 7(8): 1070-1081.

Cherkasov IA, Kravchenko NA. An improved method for the isolation of lysozymes by enzyme-substrate chromatography. Biochemistry. 1969; 34(5): 1089-1091. (in Russian)

Cherkasov IA, Kravchenko NA. Copyright certificate 178771 USSR, IPC C12K. A method for producing enzyme preparations of lysozymes from their raw materials. Application number 943666/28-13. Date of filling: 17.11.1965. Publ.: 19.11.1966. Bul. № 4. (in Russian)

Lakhtin V, Kostanova E, Arbatskii N. Purification and characterization of multiple forms of papaya latex lysozyme. Applied biochemistry and microbiology. 1995; 2: 247-254. (in Russian)

Islam R, Kite J, Baker AS [and others]. Affinity purification of hen egg lysozyme using sephadex G75. African J. of Biotechnology. 2006; 5(20): 1902-1908.

Downloads

  • PDF

Published

2019-11-29

How to Cite

1.
LEVITSKY, A. P., MUDRIK, L. M., KHROMAGINA, L. N., SEVOSTIANOVA, T. A. and SELIVANSKAIA, I. A. Lipopolysaccharide increases lysozyme adhesion to chitin. Journal of Education, Health and Sport. Online. 29 November 2019. Vol. 9, no. 11, pp. 240-247. [Accessed 5 July 2025]. DOI 10.12775/JEHS.2019.09.11.021.
  • ISO 690
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
Download Citation
  • Endnote/Zotero/Mendeley (RIS)
  • BibTeX

Issue

Vol. 9 No. 11 (2019)

Section

Research Articles

License

The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0

Stats

Number of views and downloads: 618
Number of citations: 0

Search

Search

Browse

  • Browse Author Index
  • Issue archive

User

User

Current Issue

  • Atom logo
  • RSS2 logo
  • RSS1 logo

Information

  • For Readers
  • For Authors
  • For Librarians

Newsletter

Subscribe Unsubscribe

Tags

Search using one of provided tags:

lysozyme, lipopolysaccharide, chitin, bacteriolysis, adsorption of lysozyme on chitin
Up

Akademicka Platforma Czasopism

Najlepsze czasopisma naukowe i akademickie w jednym miejscu

apcz.umk.pl

Partners

  • Akademia Ignatianum w Krakowie
  • Akademickie Towarzystwo Andragogiczne
  • Fundacja Copernicus na rzecz Rozwoju Badań Naukowych
  • Instytut Historii im. Tadeusza Manteuffla Polskiej Akademii Nauk
  • Instytut Kultur Śródziemnomorskich i Orientalnych PAN
  • Instytut Tomistyczny
  • Karmelitański Instytut Duchowości w Krakowie
  • Ministerstwo Kultury i Dziedzictwa Narodowego
  • Państwowa Akademia Nauk Stosowanych w Krośnie
  • Państwowa Akademia Nauk Stosowanych we Włocławku
  • Państwowa Wyższa Szkoła Zawodowa im. Stanisława Pigonia w Krośnie
  • Polska Fundacja Przemysłu Kosmicznego
  • Polskie Towarzystwo Ekonomiczne
  • Polskie Towarzystwo Ludoznawcze
  • Towarzystwo Miłośników Torunia
  • Towarzystwo Naukowe w Toruniu
  • Uniwersytet im. Adama Mickiewicza w Poznaniu
  • Uniwersytet Komisji Edukacji Narodowej w Krakowie
  • Uniwersytet Mikołaja Kopernika
  • Uniwersytet w Białymstoku
  • Uniwersytet Warszawski
  • Wojewódzka Biblioteka Publiczna - Książnica Kopernikańska
  • Wyższe Seminarium Duchowne w Pelplinie / Wydawnictwo Diecezjalne „Bernardinum" w Pelplinie

© 2021- Nicolaus Copernicus University Accessibility statement Shop