Morphofunctional features of endometrium in women with combination of male and female factors
Keywords
adenomyosis, endometrium, infertility, proteomic profile, estrogen-α receptor, progesterone receptor, HOXA10, Leukemia inhibitory factor, glycoprotein 130, interleukin-6Abstract
Treatment couples with a combination of male and female factor infertility is the most difficult and often requires precisely ART. However, ICSI efficacy currently does not exceed 35-40 %, about a third of all pregnancies are terminated early. The purpose of the study was to determine the morphofunctional features of endometrium in women from married couples with a combination of female and male factors of infertility. Material and methods. There were 100 married couples with prospective follow-up with a combination of male and female infertility factors of group A who had applied for assisted reproductive technologies (ART). All men in group A suffered from pathozoospermia. Control group C consisted of 34 couples with secondary infertility of tube genesis, who consisted of conditionally somatically healthy women with a history of childbirth, without fallopian tubes, and their men with normozoospermia. Group A was stratified each into 3 subgroups. The A1 subgroup (n = 32) included married couples who had chronic anovulation; in subgroup A2 (n = 37) - married couples who had tubal infertility; in subgroup A3 (n = 31) - married couples in which women had a combination of tubal and anovulatory factors of infertility. Material for morphological examination were endometrial pipel-biopsy specimens on day 21-22th of the menstrual cycle on the day of the expected implantation window. It were determined expression of HOXA10, Leukemia inhibitory factor (LIF), glycoprotein 130 (gp130), interleukin-6 (IL-6) in endometrium. Results: morphofunctional status of women's endometrium with a combination of female and male infertility factors was characterized by an increase in estrogen-α receptor IRS in the glands and endometrial stroma, respectively, 1.86 (p <0.01) and 1.37 (p<0.01) times, respectively. expression of P4 receptors in endometrial glands 1.48 times (p<0.01) against the background of their decrease in stroma by 1.39 (p<0.01); increase in infiltration of endometrial stromal CD56+ - positive natural killer cells by 10.22 times (p<0.01) and presence of CD138+ immunopositive cell cells in 66.00 % of cases. Patients with a combination of male and female infertility factors have individual changes in endometrial morphofunctional features during the intended implantation window, one only that should be considered when determining the tactics of treating women with different male forms. Conclusions. Changes in the proteomic profile of the endometrium play an important role in the occurrence of reproductive disorders and are individual depending on the combination of various of female and male factors of infertility.
References
Glynn DJ, Heng K, Russell DL, Sharkey DJ, Robertson SA, Anand-Ivell R, Ivell R. Male Seminal Relaxin Contributes to Induction of the Post-mating Cytokine Response in the Female Mouse Uterus. Front Physiol. 2017; 8: 422. doi: 10.3389/fphys.2017.00422.
Meuleman T, Snaterse G, van Beelen E, Anholts JD, Pilgram GS, van der Westerlaken LA, Eikmans M, Claas FH. The immunomodulating effect of seminal plasma on T cells. J Reprod Immunol. 2015; 110: 109-16. doi: 10.1016/j.jri.2015.01.012.
Robertson SA, Prins JR, Sharkey DJ, Moldenhauer LM. Seminal fluid and the generation of regulatory T cells for embryo implantation. Am J Reprod Immunol. 2013; 69(4): 315–30. doi: 10.1111/aji.12107.
Schjenken JE, Robertson SA.Seminal Fluid Signalling in the Female Reproductive Tract: Implications for Reproductive Success and Offspring Health. Adv Exp Med Biol. 2015;868:127-58. doi: 10.1007/978-3-319-18881-2_6.
Seylova AI, Dubinina VG. Influence of pathospermia on endometrial immune-endocrine status. Proceedings of the Association of Obstetricians-Gynecologists of Ukraine. Rivne: Estero, 2017:224-229.
Sharkey DJ, Macpherson AM, Tremellen KP, Mottershead DG, Gilchrist RB, Robertson SA. TGF-beta mediates proinflammatory seminal fluid signaling in human cervical epithelial cells. J Immunol. 2012; 189(2): 1024–35. doi: 10.4049/jimmunol.1200005.
Sharkey DJ, Tremellen KP, Jasper MJ, Gemzell-Danielsson K, Robertson SA. Seminal fluid induces leukocyte recruitment and cytokine and chemokine mRNA expression in the human cervix after coitus. J Immunol. 2012; 188(5): 2445–54. doi: 10.4049/jimmunol.1102736.
Simon L, Zini A, Dyachenko A, Ciampi A, Carrell DT. A systematic review and meta-analysis to determine the effect ofsperm DNA damage on in vitro fertilization andintracytoplasmic sperm injection outcome. Asian J Androl. 2017 Jan-Feb;19(1):80–90. doi: 10.4103/1008-682X.182822.
Tremellen KP, Seamark RF, Robertson SA. Seminal transforming growth factor beta1 stimulates granulocyte-macrophage colony-stimulating factor production and inflammatory cell recruitment in the murine uterus. Biol Reprod. 1998; 58(5): 1217–25.
Yuzyko OM. Reproductive health of women: medical and social aspects. Interview. Word and health. 2014; December 6. Access mode: http://ozdorovie.com.ua/reproduktivne-zdorov-ya-zhinki-mediko-sotsialni-aspekti /# acceptLicense.
Downloads
Published
How to Cite
Issue
Section
License
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 139
Number of citations: 0