The role of oxidative stress in the pathogenesis of type 2 diabetes mellitus against the background of obesity and determination of the efficacy of complex pathogenetic correction

Abstract

Introduction. Diabetes mellitus is currently a worldwide problem, one of the key pathogenetic links of which is the development of oxidative stress

Aim: to study the markers of oxidative stress in the pathogenesis of type 2 diabetes mellitus against the background of obesity and to determine the efficacy of complex pathogenetic correction

Materials and methods of the study: The study was carried out on white nonlinear rats of both sexes weighing 180 - 200 g. All experimental animals were divided into 5 groups. Group 1 - intact, animals kept on a standard diet and water regimen; Group 2 - animals in which type 2 diabetes mellitus was modelled. The streptozotocin model was used to reproduce type 2 DM.

Group 3 - animals with modelled diabetes mellitus and obesity. Group 4 - rats that, against the background of modelled diabetes mellitus and obesity, received hypoglycaemic therapy - metformin

Group 5 - rats that, against the background of modelled diabetes mellitus and obesity, received metformin together with daily intragastric administration of L-arginine, citicoline and vitamin E for 30 days; Group 6 - rats that, against the background of modelled diabetes mellitus and obesity, received metformin together with daily intragastric administration of L-arginine, citicoline and vitamin E for 60 days

Results: The results we obtained indicate the development of oxidative stress against the background of modelling streptozotocin-induced diabetes mellitus under the conditions of our experiment, as evidenced by an increase in both primary and secondary LPO products. An aggravating effect of obesity on the pathogenesis of experimental diabetes mellitus was established, in particular on the development of oxidative stress in this pathology, which is confirmed by an increase in the level of both diene conjugates and malondialdehyde and all TBA-active products in general in Group 3. The analysis of the data of Group 4 (in which the pathological conditions were corrected with metformin) established that correction of the pathological condition with hypoglycaemic agents has a positive result, but does not allow a pronounced correction of the disturbances of the prooxidant system, which once again confirms the need to involve additional means of correction. The results of Group 5 indicate that the inclusion of citicoline, a nitric oxide donor and vitamin E for 30 days in the correction of modelled diabetes mellitus combined with obesity markedly reduces the activity of oxidative stress, but the results do not reach normative va

lues. The obtained data indicate the efficacy of long-term use of combined correction in DM, especially if it develops against the background of comorbid pathology. It was found that changes in primary LPO products are more pronouncedly amenable to correction in the modelled pathology than the level of the representative of the secondary ones - MDA