Enzymatic method for determining in vivo the mineralizing efficiency of osteoprotective drugs

Authors

  • A. Levitsky Odessa National Technological University
  • K. Pavlenko Odessa National Medical University
  • I. Selivanska Odessa National Medical University
  • A. Lapinska Odessa National Technological University

DOI:

https://doi.org/10.12775/JEHS.2026.87.68087

Keywords

osteogenesis, mineralization, osteoprotectors

Abstract

For screening osteoprotectors, it is proposed to investigate the mineralizing activity of rat bone tissue (femur, jaws, teeth) in which experimental osteoporosis was reproduced (stress, inadequate nutrition, dysmetabolic syndrome). It is proposed to determine the mineralizing activity by the ratio of alkaline (ALP) and acid (AP) phosphatase activity in bone tissue.

It is proposed to determine in vivo the mineralizing efficiency of the drug by the percentage increase in mineralizing activity per dose of the drug in mg/kg of live weight.

Of the three osteoprotectors studied (EkSoVit, quercetin and ascorbic acid), quercetin was the most effective, and ascorbic acid was the least effective.

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Journal of Education, Health and Sport

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Published

2026-01-09

How to Cite

1.
LEVITSKY, A., PAVLENKO, K., SELIVANSKA, I. and LAPINSKA, A. Enzymatic method for determining in vivo the mineralizing efficiency of osteoprotective drugs. Journal of Education, Health and Sport. Online. 9 January 2026. Vol. 87, p. 68087. [Accessed 13 January 2026]. DOI 10.12775/JEHS.2026.87.68087.

Issue

Vol. 87 (2026)

Section

Medical Sciences

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Copyright (c) 2026 A. Levitsky, K. Pavlenko, I. Selivanska, A. Lapinska

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