Intestinal microflora on physical performance in athletes - a comprehensive review
DOI:
https://doi.org/10.12775/JEHS.2025.80.59318Keywords
intestinal microflora, microbiome, physical performance, metabolism regulation, probiotics, prebioticsAbstract
Introduction: The intestinal microflora is a crucial component of the human body, developing in parallel with the host and playing a pivotal role in maintaining health throughout life. The composition of the microbiota is dynamic, influenced by factors such as genotype, diet, and initial colonization at birth, resulting in a unique microbiotic profile for each individual. The gut microbiota supports a variety of physiological functions, including immune modulation, nutrient metabolism, and the synthesis of essential vitamins and neurotransmitters. Importantly, it maintains homeostasis, through the bidirectional communication system between intestines and central nervous system, which is necessary for all forms of physical performance.
Purpose of the work: The purpose of this study is to review the effects of intestinal microflora on physical performance and endurance in athletes. Special attention is given to the influence of dietary supplements, which can have an impact on composition of the microflora.
Materials and methods: A comprehensive analysis of research articles available on PubMed, Google Scholar, Web of Science, Embase, and Scopus was conducted using search terms related to: "intestinal microflora," "physical performance," "gut-brain axis," "athletes" and "physical training."
Results: The intestinal microflora is linked to the increased performance during physical exercise in athletes. Understanding this influence on the health and physical ability of athletes will allow better results in competitions and endurance.
References
Zhang CX, Wang HY, Chen TX. Interactions between Intestinal Microflora/Probiotics and the Immune System. Biomed Res Int. 2019 Nov 20;2019:6764919. doi: 10.1155/2019/6764919. PMID: 31828119; PMCID: PMC6886316.
Kåhrström CT, Pariente N, Weiss U. Intestinal microbiota in health and disease. Nature. 2016 Jul 7;535(7610):47. doi: 10.1038/535047a. PMID: 27383978.
Backhed F, Ley RE, Sonnenburg JL, Peterson DA, Gordon JI. Host-bacterial mutualism in the human intestine. Science. 2005;307(5717):1915–1920. doi: 10.1126/science.1104816.
Lynch SV, Pedersen O. The Human Intestinal Microbiome in Health and Disease. N Engl J Med. 2016 Dec 15;375(24):2369-2379. doi: 10.1056/NEJMra1600266. PMID: 27974040.
Kamada N, Chen GY, Inohara N, Núñez G. Control of pathogens and pathobionts by the gut microbiota. Nat Immunol. 2013 Jul;14(7):685-90. doi: 10.1038/ni.2608. PMID: 23778796; PMCID: PMC4083503.
Lazar V, Ditu LM, Pircalabioru GG, Gheorghe I, Curutiu C, Holban AM, Picu A, Petcu L, Chifiriuc MC. Aspects of Gut Microbiota and Immune System Interactions in Infectious Diseases, Immunopathology, and Cancer. Front Immunol. 2018 Aug 15;9:1830. doi: 10.3389/fimmu.2018.01830. PMID: 30158926; PMCID: PMC6104162.
Strandwitz P. Neurotransmitter modulation by the gut microbiota. Brain Res. 2018 Aug 15;1693(Pt B):128-133. doi: 10.1016/j.brainres.2018.03.015. PMID: 29903615; PMCID: PMC6005194.
Góralczyk-Bińkowska A, Szmajda-Krygier D, Kozłowska E. Oś mikrobiota-jelito-mózg w zaburzeniach psychicznych. Int J Mol Sci. 2022 24 września; 23(19):11245. doi: 10.3390/ijms231911245. Identyfikator PMID: 36232548; PMCID: PMC9570195.
Silva YP, Bernardi A, Frozza RL. The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication. Front Endocrinol (Lausanne). 2020 Jan 31;11:25. doi: 10.3389/fendo.2020.00025. PMID: 32082260; PMCID: PMC7005631.
Varghese S, Rao S, Khattak A, Zamir F, Chaari A. Physical Exercise and the Gut Microbiome: A Bidirectional Relationship Influencing Health and Performance. Nutrients. 2024 Oct 28;16(21):3663. doi: 10.3390/nu16213663. PMID: 39519496; PMCID: PMC11547208.
Nolte S, Krüger K, Lenz C, Zentgraf K. Optimizing the Gut Microbiota for Individualized Performance Development in Elite Athletes. Biology (Basel). 2023 Dec 5;12(12):1491. doi: 10.3390/biology12121491. PMID: 38132317; PMCID: PMC10740793.
Carey RA, Montag D. Exploring the relationship between gut microbiota and exercise: short-chain fatty acids and their role in metabolism. BMJ Open Sport Exerc Med. 2021 Apr 20;7(2):e000930. doi: 10.1136/bmjsem-2020-000930. PMID: 33981447; PMCID: PMC8061837.
Scheiman J, Luber JM, Chavkin TA, MacDonald T, Tung A, Pham LD, Wibowo MC, Wurth RC, Punthambaker S, Tierney BT, Yang Z, Hattab MW, Avila-Pacheco J, Clish CB, Lessard S, Church GM, Kostic AD. Meta-omics analysis of elite athletes identifies a performance-enhancing microbe that functions via lactate metabolism. Nat Med. 2019 Jul;25(7):1104-1109. doi: 10.1038/s41591-019-0485-4. Epub 2019 Jun 24. PMID: 31235964; PMCID: PMC7368972.
Petersen LM, Bautista EJ, Nguyen H, Hanson BM, Chen L, Lek SH, Sodergren E, Weinstock GM. Community characteristics of the gut microbiomes of competitive cyclists. Microbiome. 2017 Aug 10;5(1):98. doi: 10.1186/s40168-017-0320-4. PMID: 28797298; PMCID: PMC5553673.
Eda N, Tsuno S, Nakamura N, Sone R, Akama T, Matsumoto M. Effects of Intestinal Bacterial Hydrogen Gas Production on Muscle Recovery following Intense Exercise in Adult Men: A Pilot Study. Nutrients. 2022 Nov 18;14(22):4875. doi: 10.3390/nu14224875. PMID: 36432562; PMCID: PMC9693815.
Hadžić E, Starcevic A, Rupčić T, Zucko J, Čvrljak T, Renko I, Knjaz D, Novak D. Effects of Soluble Dietary Fibre on Exercise Performance and Perception of Fatigue in Young Basketball Players. Food Technol Biotechnol. 2023 Sep;61(3):389-401. doi: 10.17113/ftb.61.03.23.8124. PMID: 38022878; PMCID: PMC10666946.
Clark A, Mach N. Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes. J Int Soc Sports Nutr. 2016 Nov 24;13:43. doi: 10.1186/s12970-016-0155-6. PMID: 27924137; PMCID: PMC5121944.
Liu H, Wang J, He T, Becker S, Zhang G, Li D, Ma X. Butyrate: A Double-Edged Sword for Health? Adv Nutr. 2018 Jan 1;9(1):21-29. doi: 10.1093/advances/nmx009. PMID: 29438462; PMCID: PMC6333934.
Przewłócka K, Folwarski M, Kaźmierczak-Siedlecka K, Skonieczna-Żydecka K, Kaczor JJ. Gut-Muscle AxisExists and May Affect Skeletal Muscle Adaptation to Training. Nutrients. 2020 May 18;12(5):1451. doi: 10.3390/nu12051451. PMID: 32443396; PMCID: PMC7285193.
Patel BK, Patel KH, Lee CN, Moochhala S. Intestinal Microbiota Interventions to Enhance Athletic Performance-A Review. Int J Mol Sci. 2024 Sep 19;25(18):10076. doi: 10.3390/ijms251810076. PMID: 39337561; PMCID: PMC11432184.
Dohnalová L, Lundgren P, Carty JRE, Goldstein N, Wenski SL, Nanudorn P, Thiengmag S, Huang KP, Litichevskiy L, Descamps HC, Chellappa K, Glassman A, Kessler S, Kim J, Cox TO, Dmitrieva-Posocco O, Wong AC, Allman EL, Ghosh S, Sharma N, Sengupta K, Cornes B, Dean N, Churchill GA, Khurana TS, Sellmyer MA, FitzGerald GA, Patterson AD, Baur JA, Alhadeff AL, Helfrich EJN, Levy M, Betley JN, Thaiss CA. A microbiome-dependent gut-brain pathway regulates motivation for exercise. Nature. 2022 Dec;612(7941):739-747. doi: 10.1038/s41586-022-05525-z. Epub 2022 Dec 14. PMID: 36517598; PMCID: PMC11162758.
Souza PB, de Araujo Borba L, Castro de Jesus L, Valverde AP, Gil-Mohapel J, Rodrigues ALS. Major Depressive Disorder and Gut Microbiota: Role of Physical Exercise. Int J Mol Sci. 2023 Nov 28;24(23):16870. doi: 10.3390/ijms242316870. PMID: 38069198; PMCID: PMC10706777.
Hughes RL, Holscher HD. Fueling Gut Microbes: A Review of the Interaction between Diet, Exercise, and the Gut Microbiota in Athletes. Adv Nutr. 2021 Dec 1;12(6):2190-2215. doi: 10.1093/advances/nmab077. PMID: 34229348; PMCID: PMC8634498.
Murtaza N, Burke LM, Vlahovich N, Charlesson B, O' Neill H, Ross ML, Campbell KL, Krause L, Morrison M. The Effects of Dietary Pattern during Intensified Training on Stool Microbiota of Elite Race Walkers. Nutrients. 2019 Jan 24;11(2):261. doi: 10.3390/nu11020261. PMID: 30682843; PMCID: PMC6413084.
Cronin P, Joyce SA, O'Toole PW, O'Connor EM. Dietary Fibre Modulates the Gut Microbiota. Nutrients. 2021 May 13;13(5):1655. doi: 10.3390/nu13051655. PMID: 34068353; PMCID: PMC8153313.
Kristensen NB, Bryrup T, Allin KH, Nielsen T, Hansen TH, Pedersen O. Alterations in fecal microbiota composition by probiotic supplementation in healthy adults: a systematic review of randomized controlled trials. Genome Med. 2016 May 10;8(1):52. doi: 10.1186/s13073-016-0300-5. PMID: 27159972; PMCID: PMC4862129.
Son J, Jang LG, Kim BY, Lee S, Park H. The Effect of Athletes' Probiotic Intake May Depend on Protein and Dietary Fiber Intake. Nutrients. 2020 Sep 25;12(10):2947. doi: 10.3390/nu12102947. PMID: 32992898; PMCID: PMC7650591.
Oniszczuk A, Oniszczuk T, Gancarz M, Szymańska J. Role of Gut Microbiota, Probiotics and Prebiotics in the Cardiovascular Diseases. Molecules. 2021 Feb 22;26(4):1172. doi: 10.3390/molecules26041172. PMID: 33671813; PMCID: PMC7926819.
Holscher HD. Dietary fiber and prebiotics and the gastrointestinal microbiota. Gut Microbes. 2017 Mar 4;8(2):172-184. doi: 10.1080/19490976.2017.1290756. Epub 2017 Feb 6. PMID: 28165863; PMCID: PMC5390821.
Markowiak P, Śliżewska K. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients. 2017 Sep 15;9(9):1021. doi: 10.3390/nu9091021. PMID: 28914794; PMCID: PMC5622781.
Jäger R, Mohr AE, Carpenter KC, Kerksick CM, Purpura M, Moussa A, Townsend JR, Lamprecht M, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Smith-Ryan AE, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ter Haar JA, Antonio J. International Society of Sports Nutrition Position Stand: Probiotics. J Int Soc Sports Nutr. 2019 Dec 21;16(1):62. doi: 10.1186/s12970-019-0329-0. PMID: 31864419; PMCID: PMC6925426.
Yang K, Chen Y, Wang M, Zhang Y, Yuan Y, Hou H, Mao YH. The Improvement and Related Mechanism of Microecologics on the Sports Performance and Post-Exercise Recovery of Athletes: A Narrative Review. Nutrients. 2024 May 24;16(11):1602. doi: 10.3390/nu16111602. PMID: 38892536; PMCID: PMC11174581.
Donati Zeppa S, Agostini D, Gervasi M, Annibalini G, Amatori S, Ferrini F, Sisti D, Piccoli G, Barbieri E, Sestili P, Stocchi V. Mutual Interactions among Exercise, Sport Supplements and Microbiota. Nutrients. 2019 Dec 20;12(1):17. doi: 10.3390/nu12010017. PMID: 31861755; PMCID: PMC7019274.
Genton L, Pruijm M, Teta D, Bassi I, Cani PD, Gaïa N, Herrmann FR, Marangon N, Mareschal J, Muccioli GG, Stoermann C, Suriano F, Wurzner-Ghajarzadeh A, Lazarevic V, Schrenzel J. Gut barrier and microbiota changes with glycine and branched-chain amino acid supplementation in chronic haemodialysis patients. J Cachexia Sarcopenia Muscle. 2021 Dec;12(6):1527-1539. doi: 10.1002/jcsm.12781. Epub 2021 Sep 18. PMID: 34535959; PMCID: PMC8718035.
Day-Walsh P, Shehata E, Saha S, Savva GM, Nemeckova B, Speranza J, Kellingray L, Narbad A, Kroon PA. The use of an in-vitro batch fermentation (human colon) model for investigating mechanisms of TMA production from choline, L-carnitine and related precursors by the human gut microbiota. Eur J Nutr. 2021 Oct;60(7):3987-3999. doi: 10.1007/s00394-021-02572-6. Epub 2021 May 2. PMID: 33934200; PMCID: PMC8437865.
Maughan RJ, Burke LM, Dvorak J, Larson-Meyer DE, Peeling P, Phillips SM, Rawson ES, Walsh NP, Garthe I, Geyer H, Meeusen R, van Loon LJC, Shirreffs SM, Spriet LL, Stuart M, Vernec A, Currell K, Ali VM, Budgett RG, Ljungqvist A, Mountjoy M, Pitsiladis YP, Soligard T, Erdener U, Engebretsen L. IOC consensus statement: dietary supplements and the high-performance athlete. Br J Sports Med. 2018 Apr;52(7):439-455. doi: 10.1136/bjsports-2018-099027. Epub 2018 Mar 14. PMID: 29540367; PMCID: PMC5867441.
Brancaccio M, Mennitti C, Cesaro A, Fimiani F, Vano M, Gargiulo B, Caiazza M, Amodio F, Coto I, D'Alicandro G, Mazzaccara C, Lombardo B, Pero R, Terracciano D, Limongelli G, Calabrò P, D'Argenio V, Frisso G, Scudiero O. The Biological Role of Vitamins in Athletes' Muscle, Heart and Microbiota. Int J Environ Res Public Health. 2022 Jan 23;19(3):1249. doi: 10.3390/ijerph19031249. PMID: 35162272; PMCID: PMC8834970.
Mohr AE, Jäger R, Carpenter KC, Kerksick CM, Purpura M, Townsend JR, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ortega-Santos CP, Ter Haar JA, Arciero PJ, Antonio J. The athletic gut microbiota. J Int Soc Sports Nutr. 2020 May 12;17(1):24. doi: 10.1186/s12970-020-00353-w. PMID: 32398103; PMCID: PMC7218537.
Varghese S, Rao S, Khattak A, Zamir F, Chaari A. Physical Exercise and the Gut Microbiome: A Bidirectional Relationship Influencing Health and Performance. Nutrients. 2024 Oct 28;16(21):3663. doi: 10.3390/nu16213663. PMID: 39519496; PMCID: PMC11547208.
Mirzaei R, Dehkhodaie E, Bouzari B, Rahimi M, Gholestani A, Hosseini-Fard SR, Keyvani H, Teimoori A, Karampoor S. Dual role of microbiota-derived short-chain fatty acids on host and pathogen. Biomed Pharmacother. 2022 Jan;145:112352. doi: 10.1016/j.biopha.2021.112352. Epub 2021 Nov 26. PMID: 34840032.
Li Y, Cheng M, Zha Y, Yang K, Tong Y, Wang S, Lu Q, Ning K. Gut microbiota and inflammation patterns for specialized athletes: a multi-cohort study across different types of sports. mSystems. 2023 Aug 31;8(4):e0025923. doi: 10.1128/msystems.00259-23. Epub 2023 Jul 27. PMID: 37498086; PMCID: PMC10470055.
Bycura D, Santos AC, Shiffer A, Kyman S, Winfree K, Sutliffe J, Pearson T, Sonderegger D, Cope E, Caporaso JG. Impact of Different Exercise Modalities on the Human Gut Microbiome. Sports (Basel). 2021 Jan 21;9(2):14. doi: 10.3390/sports9020014. PMID: 33494210; PMCID: PMC7909775.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Natalia Kucharczyk, Katarzyna Półtorak, Wiktor Filarski, Zofia Markowicz, Dagmara Gaweł-Dąbrowska

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 43
Number of citations: 0