Сondition of pro-oxidant and antioxidant systems in guinea pigs’ lungs under the condition of immobilization stress.
DOI:
https://doi.org/10.12775/JEHS.2023.50.01.013Keywords
immobilization stress, peroxide lipid oxidation, antioxidant systemAbstract
We have investigated the results of alterations in indices of pro-oxidant (conjugated diene and malondialdehyde ) and antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems in guinea pigs’ lungs under the conditions of immobilization stress. The experiment was conducted on 40 female guinea pigs weighing 0.18-0.20 kg. The animals were divided into 4 groups, each contained 10 guinea pigs: I – intact guinea pigs ( control), II–guinea pigs with model of IS on1st day of experiment;Ш–animals on 2nd day of experiment;IV- group of animals on 34th day of experimental model of IS.
The results of our experimental work showed a significant accumulation of lipid peroxidation products in the lung`s tissure in different periods ( on 1st, 2nd and 34th days) of immobilization stress. The state of antioxidant defence was characterized by moderate decrease of inzymes activity (superoxide dismutase, catalase and ceruloplasmin). disorders of balance between pro-oxidant and antioxidant systems couse oxidative stress development.
References
Stress cardiomyopathy after intravenous administration of catecholamines and beta-receptor agonists / J. Abraham, J. O. Mudd, N. K. Kapur [et al.] // J. Am. Coll. Cardiol. – 2009. – Vol. 53, № 15. – P. 1320–1325.
Post-traumatic stress. The mechanisms of trauma / D. Guerreiro, B. Brito, J. L. Bartista et al // Acta Med. Port. – 2007. – Vol. 20, № 4. – P. 347–354.
Lu L. Oxidative stress in the infarcted heart : role of de novo angiotensin II production / L. Lu, M. T. Quinn, Y. Sun // Biochemical and Biophysical Research Communications. – 2004. – V. 325, № 3. – P. 943–951.
Khasky A.D., Smith J.C. Stress, relaxation states, and creativity. Percept. Mot. Skills. 2000,88 (22), 409416.
Karanth J., Jeevaratnam K. Oxidative stress and antioxidant status in rat blood, muscle: effect of dietary lipid, carnitine and exercise //bit. J. Vitam. Nutr. Res. - 2005. -V. 75, No5.-P 333-339.
Gorizontov P. D. Stress i sistema krovi / P. D. Gorizontov, O. I. Belousova, M. I. Fedotov // M.: Meditsina, 1983. – 338 s. [in Rusian]
Gavrilov A.B., Myshkorudnaya M.I. Spektrofotometricheskoye opredeleniye soderzhaniya gidroperekisey lipidov v plazme. Laboratornaya diagnostika ishemicheskoy bolezni serdtsa, - K.: Zdorov'ya, 1989,170-171[in Rusian]
Korobeynikov E.N. Modifikatsiya opredeleniya produktov POL v reaktsii s tiobarbiturovoy kislotoy Lab. delo 1989,7,8-10[in Rusian]
Fried, R. (1975). Enzymatic and non-enzymatic assay of super oxide dismutase. Biochemie, (57), 65, 657-660.
Holmes, R. & Masters, C. (1970). Epigenetic interconversions of the multiple forms of mouse liver catalase. FEBS Lett.,(11), 1, 45-48.
Kolb V. G. Opredeleniye aktivnosti tseruloplazmina v krovi / V. G. Kolb, V. S. Kamyshnikov // Spravochnik po klinicheskoy khimii.– Minsk: Bela rus', 1982.– S. 290–291. [in Rusian]
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Mariana Baida, Iryna Hot, Yuliia Antonenko
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 198
Number of citations: 0
