Experimental research of nephroprotective activity benophylline in myoglobinurical acute kidney injury in rats

Abstract

The results in this article demonstrate of the dosage of the nephroprotective activity of the newly 7,8-substituted of theophylline are shown: 7-n-methylbenzyl-8-n-bromobenzylidenhydrazinotheophylline (code name benofillin). Nephroprotective activity was supplemented by of acute renal failure, which was called one-time intramuscularly introduced to the thigh 50% water solution of glycerol. Blowing a benofillin on the function of kidneys in rats were given to the minds of the water load. Evaluation of excretory function of kidneys held for the showers of diuresis, glomerular filtration rate, creatinine concentrations, relative reabsorption of water, excretion of potassium and calcium ions, protein content in the urine and it excretion, filtration charge and reabsorption of sodium ions.

The result of the dosage was shown that when therapeutic and preventive application of benofillin the quantity of diuresis increased on 1/9 times in comparison with the pathologies. Under the action of benofillin a decrease in the level of urea in blood serum in 1/5 times. The level of creatinin in blood serum slightly increased (1.1 times compared with pathology). The level of contents of urea and creatinine in urine increased in 2.3 and 2.2 times respectively, excretion of these substances by the kidneys has grown 4.6 times that 5 times respectively, which indicates about improvement of the excretory function of the kidneys. The analysis of changes in biochemical indicators in blood and urine in rats demonstrate, hat in the treatment-and-prophylactic use of benofillin the basic parameters of the visible function of kidneys are normalized.

On the background of the treatment-and-prophylactic use of benofillin glomerular filtration rate and tubular reabsorption were increased in 4.7 and 1.1 times respectively compared with pathology. The level of sodium in blood serum not changes respectively with control pathology and intact control. The level of sodium in urine decreased in 2.4 times compared with control pathology. Filtration charge and relative reabsorption increased in 7 times each of one decreased in control pathology, relative reabsorption increased in 1.2 times, sodium excretion was has not changed. When applying benofillin the level of protein in the urine was identical to the control.

The conducted studies showed the presence of nephroprotektive activity in benofillin, what is expressed in decreasing of proteinuria, excretion of sodium in the urine and retention hyperaemia, increased glomerular filtration rate and diuresis, normalization of acid-forming function of the kidneys.