The effect of dietary fat supplements on the activity of palmitic and stearic acid desaturases based on the results of a study of the fatty acid composition of neutral lipids in blood serum and liver of rats receiving a fat-free diet
DOI:
https://doi.org/10.12775/JEHS.2022.12.01.016Keywords
fat food, fatty acid desaturase, oleic acid, palmitooleic acidAbstract
Background. Desaturase enzymes are involved in the formation of monoenoic acids from saturated fatty acids. One such enzyme is stearyl-CoA-desaturase (SCD1), which converts stearic acid to oleic acid. The aim of this work was to determine the effect of edible fats with different fatty acid compositions on SCD1 activity.
Methods. High linoleic sunflower oil (HLSO), high oleic sunflower oil (HOSO) and palm oil (PO) were used. The rats were fed for 30 days with a semi-synthetic diet that did not contain any fats (FFD) and fat diets containing 5 % of each of the above oils. In animals, lipids were extracted from serum and liver and divided into 3 fractions: neutral lipids (NL), phospholipids (PL), and free fatty acids (FFA).
The fatty acid composition of each fraction was determined by gas chromatography. The SCD18 activity was determined by the C18:1 n-9/C18:0 ‒ ratio, and the SCD16 activity was determined by the C16:1 n-7/C16:0 ratio.
Results. A higher activity of SCD16 and SCD18 was found in the NL fraction, and the activity of SCD18 significantly exceeds that of SCD16. A decrease in the content of C16:0, C16:1 and C18:0 in the NL fraction of the liver and blood serum was shown. The activity of SCD16 in blood serum and liver decreases in rats fed fat diets, while the activity of SCD18 does not decrease, and even increases with the consumption of HOSO.
Conclusions. To determine the SCD1 activity, it is advisable to use the C18:1/C18:0 ratio in terms of the level of fatty acids in the NL fraction. Fatty diet inhibits SCD16 activity, and consumption of HOSO increases SCD18 activity.
References
Titov VN, Lisitsyn DM. Fat acids. Physical chemistry, biology and medicine. Tver, Triada, 2006:672. (in Russian)
Tvrzická E, Žák A, Vecka M, Staňková B. Fatty acids in human metabolism. Physiology and maintenance. 2009;II:274-302.
Svendsen K, Olsen T, Nordstrand Rusvik TC, Ulven SM, Holven KB, Retterstøl K, Telle-Hansen VH. Fatty acid profile and estimated desaturase activities in whole blood are associated with metabolic health. Lipids in Health and Disease 2020;19:102.
Titov VN. Stearyl-coenzyme A-desaturase isozymes and the action of insulin in the light of the phylogenetic theory of pathology. Oleic fatty acid in the implementation of biological functions. Clinical laboratory diagnosis. 2013;11:16-28. (in Russian)
Solinas G, Naugler W, Galimi F, Lee MS, Karin M. Saturated fatty acids inhibit induction of insulin gene transcription by JNK-mediated phosphorylation of insulin-receptor substrates. Proc Natl Acad Sci U S A. 2006;103(44):16454-16459. doi: 10.1073/pnas.0607626103.
Lambertucci 1 RH, Hirabara SM, Silveira LDR, Levada-Pires AC, Curi R, Pithon-Curi TC. Palmitate increases superoxide production through mitochondrial electron transport chain and NADPH oxidase activity in skeletal muscle cells. J Cell Physiol. 2008;216(3):796-804. doi: 10.1002/jcp.21463.
Yuzefovych LV, Solodushko VA, Wilson GL, Rachek LI. Protection from Palmitate-Induced Mitochondrial DNA Damage Prevents from Mitochondrial Oxidative Stress, Mitochondrial Dysfunction, Apoptosis, and Impaired Insulin Signaling in Rat L6 Skeletal Muscle Cells. Endocrinology. 2012;153(1):92-100. https://doi.org/10.1210/en.2011-1442.
Levitsky AP, Markov AV, Pupin TI. Development of dysbiosis in the organism of rats receiving a high-fat diet. Journal of Education, Health and Sport. 2020;10(4):199-208. eISSN 2391-8306.
Ameliushkina VA, Arinovskii AV, Titov VN, Kaba SI, Kotkina TI, Parkhamovich R. M. Fatty acids in blood plasma and erythrocytes in the glucose tolerance test.Clinical laboratory diagnosis. 2014;4:4-11. (in Russian)
Levitsky AP, Makarenko OA, Khodakov IV. Methods to investigate fats and oils. Odessa. 2015:32. (in Russian)
Levitsky AP, Makarenko OA, Demyanenko SA. Methods of experimental dentistry. Simferopol, Tarpan, 2018:78. (in Russian)
Keyts M. Metods of lipidology. Recciving, analise and identification of lipids. Мoskva, Mir, 1975:322. (in Russian)
Khodakov IV, Tkachuk VV, Velichko VI, Levitsky AP. The fatty acids composition of liver lipids of rats which received the palm oil and lincomycin. Marine Medicine Bulletin. 2017;1(74):145-152. (in Russian)
Khodakov IV. Methods for identification and determination of the content of components of the fat mixture. Identification of falsification of fats and oils: methodological recommendations. Odessa, 2019:92. (in Russian)
Levitsky A. P., Gozhenko A. I., Velichko V. V., Selivanskaya I. A., Markov A. V., Badiuk N. S. Therapeutic and preventive effectiveness of antidisbiotic agents in rats with lipid intoxication / PharmacologyOnLine; Archives - 2021 - vol. 3 –1010-1014
Levitsky A. P., Selivanskaya I. A., Lapinskaya A. P., Pupin T. I., Badiuk N. S. Influence of fat-free, fat and sucrose diets on the indicators of lipid metabolism in rats / PharmacologyOnLine; Archives - 2021 - vol. 2 – 361-365.
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