Similar neuroendocrine and metabolic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets’ spa in healthy female rats
DOI:
https://doi.org/10.12775/JEHS.2021.11.06.035Keywords
sulfate-chloride sodium-magnesium drinking mineral waters, Truskavets’ spa, neuroendocrine and metabolic parameters, female ratsAbstract
Background. In order to expand the hydro-mineral base of Truskavets’ spa by diluting brine (130 g/L), two new sulphate-chloride sodium-magnesium mineral waters "Myroslava" (5 g/L) and "Khrystyna" (10 g/L) were created. This report is the first in a series of experimental studies of their physiological activity in line with the concepts of neuroendocrine-immune complex and functional-metabolic continuum. Materials and Methods. Experiment was performed on 50 healthy female Wistar rats 220-300 g divided into 4 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Rats of the control group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. The rats of the main groups received the water "Myroslava" and "Khrystyna". The day after the completion of the drinking course in all rats assessed the state of autonomous regulation by parameters of the HRV. The plasma levels of the hormones of adaptation were determined: corticosterone, triiodothyronine and testosterone (by the ELISA); as well as electrolytes, nitric metabolites, lipid peroxidation products and antioxidant enzymes as well as cholesterol, glucose, amylase and middle mass molecules. Most of the listed parameters of metabolism as well as 17-ketosteroids were determined in daily urine. In the adrenal glands the thickness of glomerular, fascicular, reticular and medullar zones was measured. Results. To identify exactly those parameters, the set of which three groups of animals differ significantly from each other, the information field of the registered parameters was subjected to discriminant analysis. The program included in the model 6 endocrine and 11 metabolic parameters, as well as glomerular filtration. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa have similar neuroendocrine and metabolic effects on healthy old female rats significantly different from daily water.
References
Andreyeva LI, Kozhemyakin LA, Kishkun AA. Modification of the method for determining the lipid peroxide in the test with thiobarbituric acid [in Russian]. Laboratornoye Delo. 1988; 11: 41-43.
Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva. Nauka; 1984: 221.
Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.
Dubinina YY, Yefimova LF, Sofronova LN, Geronimus AL. Comparative analysis of the activity of superoxide dismutase and catalase of erythrocytes and whole blood from newborn children with chronic hypoxia [in Russian]. Laboratornoye Delo. 1988; 8: 16-19.
Flyunt VR, Flyunt I-SS, Fil’ VM, Kovbasnyuk MM, Hryvnak RF, Popel SL, Zukow W. Relationships between caused by drinking of bioactive water Naftussya changes in urine lithogenicity and neuro-humoral-immune factors in humans with their abnormalities. Journal of Education, Health and Sport. 2017; 7(3): 11-30.
Gavrilov VB, Mishkorudnaya MI. Spectrophotometric determination of plasma levels of lipid hydroperoxides [in Russian]. Laboratornoye Delo. 1983; 3: 33-36.
Goryachkovskiy АМ. Clinical Biochemistry [in Russian]. Odesa: Astroprint; 1998: 608.
Gozhenko AI. Functional-metabolic continuum [in Russian]. J of NAMS of Ukraine. 2016; 22 (1): 3-8.
Hrytsak MV, Barylyak LG, Usyns’kyi RS, Mysula IR. Endocrine and metabolic effects of sulfate chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets’ spa in healthy female rats. In: Proceedings of the XII All-Ukrainian scientific-practical conference "Topical issues of pathology under the influence of extraordinary factors on the body". Galician Readings II (Ternopil’, October 29-30, 2020). Ternopil’; 2020: 125-127.
Hrytsan II, Gozhenko AI, Badiuk NS, Zukow W. Variants of the state of electrolyte exchange in female rates. In: Rehabilitation Medicine and Health-Resort Institutions Development. Proceedings of the 19th International Applied Research Conference (Kyїv, 11-12 December 2019). Edited by O. Gozhenko, W. Zukow. Toruń, Kyiv. 2019: 25-26.
Hrytsan II, Gozhenko AI, Badiuk NS, Zukow W. Variants of the state of electrolyte exchange in female rates. Journal of Education, Health and Sport. 2019; 9(10): 262-279.
Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika; 1989: 78-138.
Korolyuk MA, Ivanova MI, Mayorova IG, Tokarev VYe. The method for determining the activity of catalase [in Russian]. Laboratornoye Delo. 1988; 1: 16-19.
Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2015: 349.
Makarenko YeV. A comprehensive definition of the activity of superoxide dismutase and glutathione reductase in red blood cells in patients with chronic liver disease [in Russian]. Laboratornoye Delo. 1988; 11: 48-50.
Polovynko IS. Integrated quantitative estimation of neuro-endocrine manifestations of chronic stress in female rats. Experimental and Clinical Physiology and Biochemistry. 2017; 3(79): 5-10.
Polovynko IS, Zajats LM, Popovych AI, Popovych IL. Integral quantitative evaluation of neuroendocrine and immune reactions to chronic stress in male rats [in Ukrainian]. In: Pathophysiology and Pharmacy: ways of integration: Abstracts of the VII National Congress of Pathophysiologists of Ukraine with International Participation (October 5-7, 2016). Kharkiv: Nat Pharmac Univer: 182.
Polovynko IS, Zayats LM, Zukow W, Popovych IL. Neuro-endocrine-immune relationships by chronic stress at male rats. Journal of Health Sciences. 2013; 3(12): 365-374.
Polovynko IS, Zajats LM, Zukow W, Yanchij RI, Popovych IL. Quantitative evaluation of integrated neuroendocrine and immune responses to chronic stress in rats male. Journal of Education, Health and Sport. 2016; 6(8): 154-166.
Polovynko IS, Zukow W. Variety of immune responses to chronic stress in rats male. Journal of Education, Health and Sport. 2016; 6(12): 843-856.
Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-87.
Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18
Popovych IL, Gozhenko AI, Zukow W, Polovynko IS. Variety of Immune Responses to Chronic Stress and their Neuro-Endocrine Accompaniment. Scholars' Press. Riga; 2020: 172.
Tiselius HS. A biochemical basis for grouping of patients with urolithiasis. Europ Urol. 1978; 4: 241-249.
Zajats LM, Polovynko IS, Zukow W. Features neuro-endocrine support diversity of immune responses to chronic stress in male rats. Journal of Education, Health and Sport. 2017; 7(3): 97-105.
Zajats LM, Polovynko IS, Zukow W, Yanchij RI, Mysakovets’ OG, Mel’nyk OI, Hrytsak YaL. Neuroendocrine-immune relatioships in rats females. Journal of Education, Health and Sport. 2017; 7(10): 59-78.
Zavidnyuk YV, Mysula IR, Klishch IM, Zukow W, Popovych IL, Korda MM. General non-specific metabolic, neuroendocrine and immune reactions to various water-salt loads in female rats. Journal of Education, Health and Sport. 2018; 8(3): 513-524.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Myroslava Hrytsak, Daria Popovych, Nataliya Badiuk, Ivanna Hrytsan; Walery Zukow
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 270
Number of citations: 0