Features of the intestinal microbiota functional status in early-aged children with rotavirus infection
DOI:
https://doi.org/10.12775/JEHS.2021.11.10.014Keywords
rotavirus infection, early-aged children, gut microbiota, short-chain fatty acidsAbstract
The aim is to assess the intestinal microflora functional and structural disorders in early-aged children in the dynamics of rotavirus infection by studying intestinal metabolites in faeces – short-chain fatty acids (SCFA).
Materials and methods. The study included 60 breastfed children aged 1-24 months with rotavirus infection (the study group) and 30 healthy children, representative by age and sex (the comparison group). Determination of SCFA (acetate, propionate, butyrate) in faeces was carried out in all children of the study group three times in the dynamics of the disease (on the 3rd, 5th and 10th day) and in healthy children once.
Results. The total concentration of SCFA in children with rotavirus infection was 3 and 2.2 times lower than in healthy children in the acute period of the disease (p<0.01 on the 3rd and 5th day, respectively), increasing on the 10th day (p<0.05), however, not reaching the normal level (p<0.01). The decrease in the total pool of SCFA occurred due to all volatile acids (C2, C3, C4), the concentrations of which were lower than in healthy children (p<0.01). Violation of the volatile acids ratio in their general pool was observed from the first days of rotavirus gastroenteritis in the form of an increase in the C2 relative concentration (p˂0.05) and a decrease in C3 and C4 profiles (p˂0.05). Correspondingly, a decrease in anaerobic index was noted. It was equal to 0.04 [0.01; 0.11] on the 3rd and 5th day of the disease, constituting only 1/5 of the healthy children values (p˂0,01), increasing on the 10th day to 0,09 [0,02; 0.17], however, remaining twice as lower than in children of the comparison group (p˂0,01).
Conclusions. There is a violation of the intestinal microflora functional condition in early-aged children from the first days of rotavirus infection, which is expressed by depletion of the total pool of SCFA and concentrations of each of them, as well as structural disorders of intestinal microbiocinosis in the form of reducing its anaerobiosis. These changes are most pronounced during the first five days of rotavirus gastroenteritis and last up to 10th day of illness.
References
Mathew, S., Smatti, M. K., Al Ansari, K., Nasrallah, G. K., Al Thani, A. A., & Yassine, H. M. (2019). Mixed Viral-Bacterial Infections and Their Effects on Gut Microbiota and Clinical Illnesses in Children. Scientific reports, 9(1), Article 865. https://doi.org/10.1038/s41598-018-37162-w
Nezhoda, I. I., Naumenko, O. M., & Nikulchenko, O. V. (2019). Letki zhyrni kysloty yak metabolichni markery porushennia mikrobiotsenozu kyshechnyku pry rotavirusnii infektsii u ditei [Volatile fatty acids as metabolic markers of intestinal microbiocenosis in children with rotavirus infection]. Infektsiini khvoroby, (3), 24–32. https://doi.org/10.11603/1681-2727.2019.3.10632 [in Ukrainian].
Meskina, E. R. (2015). Sindrom mal'absorbtsii uglevodov u detei s virusnym gastroenteritom [Carbohydrate malabsorption syndrome in children with viral gastroenteritis]. Al'manakh klinicheskoi meditsiny, (42), 79-86. https://doi.org/10.18786/2072-0505-2015-42-79-86 [in Russian].
Sohail, M. U., Al Khatib, H. A., Al Thani, A. A., Al Ansari, K., Yassine, H. M., & Al-Asmakh, M. (2021). Microbiome profiling of rotavirus infected children suffering from acute gastroenteritis. Gut pathogens, 13(1), Article 21. https://doi.org/10.1186/s13099-021-00411-x.
Engevik, M. A., Banks, L. D., Engevik, K. A., Chang-Graham, A. L., Perry, J. L., Hutchinson, D. S., Ajami, N. J., Petrosino, J. F., & Hyser, J. M. (2020). Rotavirus infection induces glycan availability to promote ileum-specific changes in the microbiome aiding rotavirus virulence. Gut microbes, 11(5), 1324–1347. https://doi.org/10.1080/19490976.2020.1754714.
Kim, A. H., Hogarty, M. P., Harris, V. C., & Baldridge, M. T. (2021). The Complex Interactions Between Rotavirus and the Gut Microbiota. Frontiers in cellular and infection microbiology, 10, Article 586751. https://doi.org/10.3389/fcimb.2020.586751.
Ermolenko K.D., Gonchar N.V., Lobzin Yu.V. (2020). Mehanizmy formirovaniya postinfekcionnoj patologii u detej - rekonvalescentov rotavirusnoj infekcii. [Post-infectious gastroenterological pathology’s mechanisms in children with rotavirus infection]. Zhurnal infektologii. 12(5). 56-61. https://doi.org/10.22625/2072-6732-2020-12-5-56-61 [in Russian].
Nogal, A., Valdes, A. M., & Menni, C. (2021). The role of short-chain fatty acids in the interplay between gut microbiota and diet in cardio-metabolic health. Gut microbes, 13(1), 1–24. https://doi.org/10.1080/19490976.2021.1897212.
Ríos-Covián, D., Ruas-Madiedo, P., Margolles, A., Gueimonde, M., de Los Reyes-Gavilán, C. G., & Salazar, N. (2016). Intestinal Short Chain Fatty Acids and their Link with Diet and Human Health. Frontiers in microbiology, 7, Article 185. https://doi.org/10.3389/fmicb.2016.00185.
Bridgman, S. L., Azad, M. B., Field, C. J., Haqq, A. M., Becker, A. B., Mandhane, P. J., Subbarao, P., Turvey, S. E., Sears, M. R., Scott, J. A., Wishart, D. S., Kozyrskyj, A. L., & CHILD Study Investigators (2017). Fecal Short-Chain Fatty Acid Variations by Breastfeeding Status in Infants at 4 Months: Differences in Relative versus Absolute Concentrations. Frontiers in nutrition, 4, Article 11. https://doi.org/10.3389/fnut.2017.00011
Reichardt, N., Duncan, S. H., Young, P., Belenguer, A., McWilliam Leitch, C., Scott, K. P., Flint, H. J., & Louis, P. (2014). Phylogenetic distribution of three pathways for propionate production within the human gut microbiota. The ISME journal, 8(6), 1323–1335. https://doi.org/10.1038/ismej.2014.14.
Oliphant, K., Allen-Vercoe, E. (2019). Macronutrient metabolism by the human gut microbiome: major fermentation by-products and their impact on host health. Microbiome, 7, Article 91. https://doi.org/10.1186/s40168-019-0704-8.
He, J., Zhang, P., Shen, L., Niu, L., Tan, Y., Chen, L., Zhao, Y., Bai, L., Hao, X., Li, X., Zhang, S., & Zhu, L. (2020). Short-Chain Fatty Acids and Their Association with Signalling Pathways in Inflammation, Glucose and Lipid Metabolism. International journal of molecular sciences, 21(17), Article 6356. https://doi.org/10.3390/ijms21176356.
Azagra-Boronat, I., Massot-Cladera, M., Knipping, K., Van't Land, B., Tims, S., Stahl, B., Knol, J., Garssen, J., Franch, À., Castell, M., Pérez-Cano, F. J., & Rodríguez-Lagunas, M. J. (2019). Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats. Cells, 8(8), Article 876. https://doi.org/10.3390/cells8080876.
Vorobiova N.V., Usachova O.V. (2021). Laboratorni oznaky malabsorbtsii vuhlevodiv u ditei rannoho viku z rotavirusnoiu infektsiieiu [Laboratory signs of carbohydrate malabsorption in early age children with rotavirus infection]. Patolohiia. 18(1). 72-79. https://doi.org/10.14739/2310-1237.2021.1.228925 [in Ukrainian].
Ivanko O. H., Bondarenko V. M. (2021). Klasternyi analiz prychyn hostrykh diarei u ditei rannoho viku, hospitalizovanykh v infektsiine viddilennia [Cluster analysis of the acute diarrhea causes in young children admitted to the infectious diseases unit]. Patolohiia. 18(2). 196-202. https://doi.org/10.14739/2310-1237.2021.2.229500 [in Ukrainian].
He, T., Priebe, M.G., Vonk, R.J., & Welling, G.W. (2005). Identification of bacteria with beta-galactosidase activity in faeces from lactase non-persistent subjects. FEMS microbiology ecology, 54(3), 463-469 .
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