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Journal of Education, Health and Sport

Rat skin inflammation markers change in response to calcium hydroxyapatite and hyaluronic acid administration
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  • Rat skin inflammation markers change in response to calcium hydroxyapatite and hyaluronic acid administration
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Rat skin inflammation markers change in response to calcium hydroxyapatite and hyaluronic acid administration

Authors

  • S. Larkina Odessa National Medical University
  • A. Seletskaya Kings College
  • O. Makarenko I. I. Mechnikov National University
  • R. Vastyanov Odessa National Medical University

DOI:

https://doi.org/10.12775/JEHS.2021.11.08.057

Keywords

calcium hydroxyapatite, hyaluronic acid, skin, markers of skin inflammation, skin cell membrane factors, connective tissue, collagen, involutive skin changes, skin aging, anty-age

Abstract

In the research, the level of some markers of skin inflammation in Wistar rats was examined after injection of calcium hydroxyapatite and hyaluronic acid drugs of various concentrations, where the nature, degree, extent of tissue damage and the general condition of the animals in general were close to humans’. Each drug was evaluated according to several indicators (MDA content, activity of neutrophil elastase, acid phosphatase, and catalase) to understand the measure of the adequacy of the body's response to the intensity of the stimulus.

A biochemical analysis of the skin of laboratory rats after the injection of the studied drugs showed that the safest drugs contain nonstabilized 4% hyaluronic acid. Either in pure form or in a 1: 1 mixture with calcium hydroxyapatite at a concentration of 55.7%, since in 2 months after their injection in the skin of experimental animals, markers of inflammation (elastase, acid phosphatase and MDA levels), catalase and API activity were found at the normal level.

The injection of drugs containing stabilized 2% hyaluronic acid and calcium hydroxyapatite 55.7%, as well as stabilized 2.6% hyaluronic acid and calcium hydroxyapatite at a concentration of 1% caused prolonged LPO activation, increased level of inflammation markers and decreased API.

The injection of calcium hydroxyapatite drugs without hyaluronic acid, even at a 1: 1 dilution with saline, also caused prolonged LPO activation, an increased level of inflammation markers and a decreased API, and, in addition, revealed a significant increase in acid phosphatase activity, which is an indicator of the integrity of cell membranes.

The obtained results made it possible to analyze the features of the molecular response and regulation of inflammation upon injection of calcium hydroxyapatite and hyaluronic acid drugs of different concentrations, which will help in prescribing of treatment.

References

Tatuzyan E.G., Belovol A.N., Tkachenko S.G. Complications of minimally invasive procedures in cosmetology. Dermatology and Venereology. 2015; 2 (64): 79-84 [In Russian].

Karpova E.I., Potekaev N.N., Murakov S.V., Danishchuk OI, Demina O.M. Complications of contouring injection plastic of the face: world and Russian experience. Plastic surgery and aesthetic medicine. 2019; 4: 54-75 [In Russian].

Wismont F.I. Inflammation. Pathophysiological aspects. Minsk, 2006: 48 [In Russian].

Stalnaya I.D, Garishvili T.G. Modern methods in biochemistry. M : Medicine, 1977: 66 - 68. [In Russian].

Karolyuk M.A., Ivanova L.I., Mayorova N.T., Tokarev K.E. Method for determining the activity of catalase. Laboratory work. 1988; 1: 16-18. [In Russian].

Levitsky A.P, Pochtar V.M., Makarenko O.A., Gridina L.I. Antioxidant-prooxidant index of blood serum of rats with experimental stomatitis and its correction with dental elixirs. Odessa Medical Journal. 2006; 1 (93): 22-25 [In Ukrainian].

Lapach S.N., Chubenko A.V., Babich P.N. Statistical methods in biomedical research using Excel. К : Morion, 2000: 320 [In Russian].

Larkina S., Makarenko O., Seletskaya A. Study of the anti-inflammatory activity of hyaluronic acid preparations. News of aesthetic medicine. 2019; 6 (118): 58-63 [In Russian].

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Published

2021-08-31

How to Cite

1.
LARKINA, S., SELETSKAYA, A., MAKARENKO, O. & VASTYANOV, R. Rat skin inflammation markers change in response to calcium hydroxyapatite and hyaluronic acid administration. Journal of Education, Health and Sport [online]. 31 August 2021, T. 11, nr 8, s. 510–519. [accessed 23.3.2023]. DOI 10.12775/JEHS.2021.11.08.057.
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Issue

Vol. 11 No. 8 (2021)

Section

Research Articles

License

Copyright (c) 2021 S. Larkina, A. Seletskaya, O. Makarenko, R. Vastyanov

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0

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