Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis
Keywords
peritonitis, experimental research, von Willebrand factor, erythrocyte intoxication index (EII), leukocyte intoxication index (LII), L-argininAbstract
The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups:
Group 1 - 20 intact animals.
Group 2 - 50 rats with simulated fecal peritonitis.
Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution.
Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor.
Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826).
The following results were obtained.
Endothelial dysfunction is the trigger mechanism of vascular catastrophes after experiencing experimental peritonitis.
Evidence of endothelial functional status impairment during experimental peritonitis is a significant increase in the level of Willebrand factor in the animals blood flow (p <0.001).
It was revealed significant increase in erythrocyte intoxication index (EII).
It has been proved increase in leukocyte intoxication index (LII) in experimental animals during simulated peritonitis.
It has been confirmed effectiveness of nitric oxide donor use in the complex correction of peritonitis and endothelial dysfunction as its complication is confirmed.
References
Kumar S, Kumar S, Kumar S, Gautam S. Spontaneous gallbladder perforation in a patient of situs inversus totalis, misdiagnosed as perforation peritonitis due to gas under the right dome of the diaphragm. BMJ Case Rep [Internet]. 2015 Jun [cited 2017 Sep 22]; 2015:1-3. Available from: https://www.ncbi.nlm.nih.gov/labs/articles/26123454/ doi: 10.1136/bcr-2014-208003
Riché FC, Dray X, Laisné MJ, Matéo J, Raskine L, Sanson-Le Pors MJ, et al. Factors associated with septic shock and mortality in generalized peritonitis: comparison between community-acquired and postoperative peritonitis. Critical Care [Internet]. 2009 [cited 2017 Sep22];13(3):R99. Available from: https://www.ncbi.nlm.nih.gov/pubmed/19552799 doi:10.1186/cc7931
Kim T, Hong SI, Park SY, Jung J, Chong YP, Kim SH, et al. Clinical Features and Outcomes of Spontaneous Bacterial Peritonitis Caused by Streptococcus pneumoniae: A Matched Case-Control Study. Medicine (Baltimore) [Internet]. 2016 May [cited 2017 Sep 22]; 95(22): e3796. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900721/ doi: 10.1097/ MD.0000000000003796
Bektas H, Kleine M, Tamac A, Klempnauer J, Schrem H. Clinical Application of the Hanover Classification for Iatrogenic Bile Duct Lesions. HPB Surg [Internet]. 2011 [cited 2017 Sep 22]; 2011:10p. Available from: https://www.hindawi.com/journals/hpb/2011/612384/ doi: 10.1155/2011/612384
Mazuski JE, Solomkin JS. Intra-abdominal infections. Surg. Clin. N. Am. 2009;89;2:421-437.
Sartelli M. A focus on intra-abdominal infections. World J. Emerg Surg. 2010;19:5-9.
Kupreeva MS, Petrosjan JeA, Suhinin AA i dr. Ocenka sostojanija krasnoj krovi pri zhelchnom peritonite. Bjulleten' Volgogradskogo nauchnogo centra RAMN. 2008;2:49-51.
Savel'ev VS, Petuhov VA, An ES, Semenov ZhS, Mironov AV. Disfunkcija jendotelija pri lipidnom distress–sindrome i dismetabolicheskih posledstvijah peritonita. RMZh. 2009;14:881
Lazarenko V.A., Lipatov V.A., Blinkov Ju.Ju., Skorikov D.V. Jeksperimental'naja model' rasprostranennogo kalovogo peritonita. Kurskij nauchno-prakticheskij vestnik "Chelovek i ego zdorov'e", 2008, № 4.S.128-132.
Pokrovskij M.V., Pokrovskaja T.G., Korchakov V.I. i dr. Jendotelioprotektornye jeffekty L-arginina pri modelirovanii deficita okisi azota. Jeksperim. i klin. farmakol. 2008; 71 (2): 29–31.
Dinamіka pokaznikіv endogennoї іntoksikacії u shhurіv rіznogo vіku za umov gostrogo otruєnnja toksinami blіdoї poganki / І. P. Kuz'mak, І. M. Klіshh, O. Z. Jaremchuk // Naukovij vіsnik Uzhgorods'kogo unіversitetu. Serіja : Bіologіja. – 2012. – Vip. 33. – S. 154–155.
Kal'f-Kalif Ja. Ja. O lejkocitarnom indekse intoksikacii i ego prakticheskom znachenii // Vrachebnoe delo.— 1941.— № 1.— S. 31–33.
Harlamova U.V.,Il'icheva O. E. Diagnosticheskoe znachenie laboratornyh markerov povrezhdenija jendotelija u bol'nyh na programmnom gemodialize// Klinicheskaja laboratornaja diagnostika.- 2012.-№ 7.-S.39-41.
Barkagan Z.S., Momot A.P. Diagnostika i kontroliruemaja terapіja narushenij gemostaza.. M. Izd. «N'judiamed». 2001. 296 s.
Downloads
Published
How to Cite
Issue
Section
License
The periodical offers access to content in the Open Access system under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0
Stats
Number of views and downloads: 162
Number of citations: 0
