Dynamics of endothelial and inducible synthase nitric oxide in experimental osteoarthritis and its correction

Abstract

Study have been carried out on white Wistar line rats (age – 3 months, weight – 180-220 g). According to the tasks the animals were divided into 7 groups:

1st group is intact (n = 20). 2nd group is rats, which were modeled osteoarthritis without further correction and were withdrawn from the experiment in the first stage (7th day) (n=40). 3rd group is rats, which were modeled osteoarthritis without further correction and removed from the experiment in the second stage (21st day) (n=40). 4th group is rats, in which experimental osteoarthritis was corrected with nonsteroidal anti-inflammatory drugs (NSAIDs) (Diclofenac) and aminoguanidine and removed from the experiment in the first stage (7th day) (n=20). 5th group is rats, in which experimental osteoarthritis was corrected with NSAIDs (Diclofenac) and aminoguanidine and withdrawn from the experiment in the second stage (21st day) (n=20). 6th group is rats, where experimental osteoarthritis was corrected using NSAIDs and a 7% L-arginine solution and withdrawn from the experiment in the first stage (7th day) (n=20)

7th group is rats, in which experimental osteoarthritis was corrected with NSAIDs and 7% L-arginine solution and withdrawn from the experiment in the second stage (21st day) (n=20)

Animals were withdrawn from the experiment for the 7th day and the 21st day after the simulation of the pathological condition. NSAIDs (Diclofenac), aminoguanidine and L-arginine were administered from the beginning of the study.

During the experiment was found nitric oxide hyperproduction by increasing the activity of inducible NO synthase. It was found decreased endothelial NO synthase activity against the background of experimental osteoarthritis development and the induced inducible NO synthase activation. It has been proven aminoguadine correction effectiveness (inducible NO-synthase inhibitor) of endothelial dysfunction in osteoarthritis. It has been established the feasibility of using L-arginine as a corrective agent for endothelial dysfunction in experimental osteoarthritis. Correction agents comparative characteristics showed that the use of nitric oxide donor is more effective compared to  inducible NO synthase inhibition.