Лечебно-профилактическое действие мукозо-адгезивных пленок с лизоцимом при токсическом стоматите = Therapeutic-prophylactic action of mucoadhesive films with lysozyme on toxic stomatite

Abstract

Dvulit I. P. Лечебно-профилактическое действие мукозо-адгезивных пленок с лизоцимом при токсическом стоматите = Therapeutic-prophylactic action of mucoadhesive films with lysozyme on toxic stomatitis. Journal of Education, Health and Sport. 2015;5(4):169-176. ISSN 2391-8306. DOI: 10.5281/zenodo.16698

http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%284%29%3A169-176

https://pbn.nauka.gov.pl/works/553807

http://dx.doi.org/10.5281/zenodo.16698

Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011 – 2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive

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Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.

The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).

© The Author (s) 2015;

This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland

Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,

provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License

(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.

This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial

use, distribution and reproduction in any medium, provided the work is properly cited.

The authors declare that there is no conflict of interests regarding the publication of this paper.

Received: 20.02.2015. Revised 27.03.2015. Accepted: 05.04.2015.

УДК 577.15 (088.8)

ЛЕЧЕБНО-ПРОФИЛАКТИЧЕСКОЕ ДЕЙСТВИЕ
МУКОЗО-АДГЕЗИВНЫХ ПЛЕНОК С ЛИЗОЦИМОМ ПРИ ТОКСИЧЕСКОМ СТОМАТИТЕ

THERAPEUTIC-PROPHYLACTIC ACTION OF MUCOADHESIVE FILMS WITH LYSOZYME ON TOXIC STOMATITIS

И. П. Двулит

I. P. Dvulit

Львовский национальный медицинский университет им. Данилы Галицкого (Украина)

 Lviv national medical university

e-mail: flavan@mail.ru

Abstract

Introduction. Mucoadhesive films (MAF), containing some biologically active substance, have some advantaged over the other dosage forms, because they provides long-term local action of medicinal means with usage of minimal quantity of acting substance. Formerly we had shown the effectiveness of MAF with immobilized lysozyme usage, possessing the anti-inflammatory and antibacterial effect on the rats oral cavity mucous membrane (OCMM) after application of bee venom.

Materials and methods. Experiments were conducted on Wister strain rats. In the homogenates of OCMM the elastase activity, malonic dialdehyde, urease, lysozyme and catalase activity were measured; the disbiosis degree and antioxidative were determined.

Results and conclusions. The medocobiologic properties of MAF based on polyvinyl alcohol cryogel with immobilized lysozyme and preservatives, of complex action, were investigated. It was shown, the application of lipopolysaccharide on oral cavity mucous membranes of Wister strain rats induced inflammation development disbiosis, decreasing of antioxidant defence. It was established, that MAF with entraped lysozyme, chlorhexidine bigluconate and ethylenediamine letraacetate, promotes 50 % decrease of urease level-marker of microbial dissemination of rats cheek mucous membrane. Application on rats oral cavity mucous membranes of lysozyme-containing films, decreases both disbiosis and inflammation degree.

Key words: LPS, stomatitis, mucoadhesive films, lysozyme, enzymes.

         Реферат

Аппликация на слизистую полости рта липополисахарида вызывает развитие воспаления, дисбиоза и снижение уровня антиоксидантной защиты. Наложение пленок, содержащих лизоцим, снижает степень дисбиоза и воспаления.

Ключевые слова: ЛПС, стоматит, мукозо-адгезивные пленки, лизоцим, ферменты.