Analysis of preparations containing caAeine for use by athletes
DOI:
https://doi.org/10.12775/HERB.2025.009Keywords
caffeine, supplements for athletes, antioxidant propertiesAbstract
Caffeine is a popular and common ingredient in the human diet. A recent study has revealed that 90% of the global adult population consumes caffeine on a daily basis. In European countries, the daily intake of caffeine ranges from 280 to 490 milligrams. The predominant source of caffeine in the global diet is coffee. However, there has been a signicant expansion in the range of products containing caffeine, including medicines and dietary supplements, which has led to widespread and uncontrolled consumption. A salient issue pertains to the regulation of active ingredient content in dietary supplements, a pivotal aspect in ensuring their safe usage. The objective of this study was to ascertain the caffeine and total polyphenol (TP) content in widely consumed products aimed at athletes, with a view to demonstrating adherence to the composition declared by the manufacturer. A selection of five products
with dierent formulations was analysed: a drink, shots and gels. The analysis of these preparations demonstrated that the choice of method of composition testing is significantly impacted by the dieffrent formulations of the products. Compliance with the content of the active substance (caffeine) as stated by the manufacturer was obtained in three out of the five preparations analysed. The preparations also differed in their total polyphenol content, which correlated with the composition of preparations with additional ingredients such as guarana seed extract.
References
[1] Frankowski M., Kowalski A., Ociepa A., Siepak J., Niedzielski P., Kofeina w kawach i ekstraktach kofeinowych i odkofeinowanych dostępnych na polskim rynku, Bromatologia i Chemia Toksykologiczna, 2008, 1(41), s. 21–27.
[2] Bojarowicz H., Przygoda M., Caffeine. Part I. Common use of caffeine and its effect on human organism, Problemy Higieny i Epidemiologii, 2017, 98(3), s. 285–289.
[3] Loftifield E., Freedman N.D., Dodd K.W., Vogtmann E., Xiao Q., Sinha R., Graybeard B., Coffee Drinking Is Widespread in the United States, but Usual Intake Varies by Key Demographic and Lifestyle Factors, The Journal of Nutrition, 2016, 146(9),
s. 1762–1768.
[4] Fanarioti V., Europe vs the US: Breaking down differences in coffee consumption, 2023, https://perfectdailygrind.com/2023/06/europe-vs-us-coffee-consumption-trends/
[5] Rejestr produktów objętych powiadomieniem o pierwszym wprowadzeniu do obrotu. Państwowa Inspekcja Sanitarna, https://www.gov.pl/web/gis/elektroniczny-system-powiadomien-powiadomienie-o-wprowadzeniu-po-raz-pierwszy-do-obrotu-suplementy-diety-zywnosc-wzbogacana-zywnosc-dla-okreslonych-grup.
[6] Dworzański W., Burdan F., Szumiło M., Jaskółka A., Anielska E., Kawa i kofeina – wrogowie czy sprzymierzeńcy kardiologa?, Kardiologia Polska, 2011, 69(2), s. 173–176.
[7] Bilondi H.T., Valipour H., Khoshro S., Jamilian P., Ostadrahimi. A., Zarezadeh M., The effect of caffeine supplementation on muscular strength and endurance: A meta-analysis of meta-analyses, Heliyon 2024, 10(15), s. 35025.
[8] Ruiz-Moreno C., Gutiérrez-Hellin J., Lara B., Del Cost J., Effect of caffeine on muscle oxygen saturation during short-term all-out exercise: a double-blind randomized crossover study, European Journal of Nutrition, 2022, 61, s. 3109–3117.
[9] Ferré S., Role of the Central Ascending Neurotransmitter Systems in the Psychostimu-lant Effects of Caffeine, Journal of Alzheimer’s Disease, 2010, 20, s. 35–49.
[10] Tarnopolsky M.A., Caffeine and Creatine Use in Sport, Annals of Nutrition & Metabolism, 2011, 57 (Suppl. 2), s. 1–8.
[11] Nakabayashi H., Hashimoto T., Ashida H., Nishiumi S., Kanazawa K., Inhibitory effects of caffeine and its metabolites on intracellular lipid accumulation in murine 3T3-L1 adipocytes, BioFactors, 2009, 34(4), s. 293–302.
[12] EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) (2011) Scientific Opinion on the substantiation of health claims related to caffeine and increased fat oxidation leading to a reduction in body fat mass (ID 735, 1484), increased energy expenditure leading to a reduction in body weight (ID 1487), increased alertness (ID 736, 1101, 1187, 1485, 1491, 2063, 2103) and increased attention (ID 736, 1485, 1491, 2375) pursuant to Article 13(1) of Regulation (EC) No 1924/20061, EFSA Journal, 2011, 9(4), s. 2054.
[13] Wierzejska R., Kofeina – powszechny składnik diety i jej wpływ na zdrowie, Roczniki Państwowego Zakłady Higieny, 2012, 63(2), s. 141–147.
[14] Addicott M.A., Laurienti P.J., A comparison of the effects of caffeine following abstinence and normal caffeine use, Psychopharmacology, 2009, 207(3), s. 423–431.
[15] Antonio J., Newmire D.E., Stout J.R., Antonio B., Gibbons M., Lowery L.M., Harper J., Willoughby D., Evans C., Anderson D., Goldstein E., Rojas J., Monsalves-Álvarez M., Forbes S.C., Lopez J.G., Ziegenfuss T., Moulding B.D., Candow D., Sagner M., Arent S.M., Common questions and misconceptions about caffeine supplementation: what does the scientific evidence really show?, Journal of the International Society of Sports Nutrition, 2024, 21(1), s. 2323919.
[16] Anikiel S., Zastosowanie spektrofotometrii UV-Vis oraz chromatografii cienkowarstwowej w analizie wybranych związków fenolowych i alkaloidów purynowych o właściwościach redukujących, 2007, praca daktorska.
[17] Takayanagi T., Nishiuchi M., Ousaka M., Oshima M., Motomizu S., Monitoring of vitamin C species in aqueous solution by flow injection analysis coupled with an on-line separation with reversed-phase column, Talanta, 2009, 79(4), s. 1055–1060.
[18] Macrae R., Nitrogenous Components, [w:] Coffee, vol 1, Chemistry, (red.) R.J. Clarke, R. Macare, wyd. Elsevier Applled Science, London, New York 1985, s. 115–152.
[19] Andrews K.W., Schweitzer A., Zhao C., Holden J.M., Roseland J.M., Brandt M., Dwyer J.T., Picciano F., Saldanha L.G., Fisher K.D., Yetley E., Betz J.M., Douglass L., The caffeine contents of dietary supplements commonly purchased in the US: analysis of 53 products with caffeine-containing ingredients, Analytical and Bioanalytical Chemistry, 2007, 389, s. 231–239.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Alicja Chrzanowska, Julia Makulec, Katarzyna Paradowska

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Stats
Number of views and downloads: 124
Number of citations: 0