Preliminary experimental research of phytotea “ATINE”

Abstract

Background and aim. Earlier we shown that phytotea “ATINE” enhances the immunomodulatory effect of adaptogenic factors of the Truskavets’ Spa in patients after radical treatment of oncological pathology as well as has physiologically beneficial effects on the neuro-endocrine-immune complex and metabolism of patients with maladaptation. The purpose of this study is to find out fundamental effects of “ATINE” in controlled experiments on rats. 

Material and methods. The experiments were conducted on 42 outbred white female rats weighing 170 - 250 g. In the first experiment, the object of the study was the weight of the body and individual organs, the activity of AlT and AsT as markers of cytolysis, the levels of erythrocytes, hemoglobin and leukocytes in the blood as well as leukocytogram at intact rats ant treated by daily water or “ATINE” infusion. In the second experiment with a similar design, an open field test and immune test was performed. In the third experiment, the subjects of the study were plasma levels of lipid peroxidation products and the weight of the adrenal glands, thymus, and spleen in intact rats and rats exposed to acute heat stress while consuming daily water or “ATINE”.

Results. We first of all stated the absence of general toxic effects of ATINE during 28 days of use. Judging by the significant Cohen’s effect sizes d_s of the registered variables, the antioxidant effect of “ATINE” is 3.37±0.13; stress-limiting 2.97±0.75; erythropoietic 2.67±0.43; neurostimulating 1.95±0.32 and immunostimulating 1.78±0.30.

Conclusion. Phytotea “ATINE” exhibits significantly pronounced classic adaptogen properties.