Effectiveness of selected strains of entomopathogenic nematodes in peat substrate in container breeding

Joanna Matuska-Łyżwa

DOI: http://dx.doi.org/10.12775/ecoq-2013-0007


Pest control in urban green areas is particularly difficult due to the limitation of pesticide use. Using efficient methods for biological control of herbivores there may be a good solution. Planting plants growing in the forest container breeding have a better growth and better root system comparing to the seedlings produced in traditional forest nurseries. Such plants are not subject to significant shock when replanted, however, they are still exposed to pests living near plantings. Our research assessed using entomopathogenic nematodes to be introduced into the soil used for plantings in order early protect seedlings against their pests. Nematodes isolated from the natural environment as well as, for control samples, from biopreparations were used in the study. The research was conducted on the soil substrate in containers with seedlings. During the experiment were taken the following parameters: insect mortality, nematode invasion extensity, migration time of invasive larvae, number of migrating larvae, and their survival. The results showed that entomopathogenic nematodes isolated from various provinces in Poland were effective, but characterized by a diverse insecticidal activity in a peat substrate.


entomopathogenic nematodes, Steinernema feltiae, Steinernema kraussei, Galleria mellonella, Owinema, Nemasys L.

Full Text:



Adams B. J., Fodor A., Koppenhofer H. S., Stackebrandt E., Stock S. P. & Klein M. G., 2006, Biodiversity and systematics of nematode-bacterium entomopathogens, Biological Control: Theory and Applications in Pest Management 37(1): 32-49. DOI 10.1016/j.biocontrol.2005.11.008

Bedding R. A. & Akhurst R. J., 1975, A simple technique for the detection of insect parasitic rhabditid nematodes in soil, Nematologica 21: 109-110.

Brzeski M. & Sandner H., 1974, Zarys nematologii [Outline of nematology], PWN Warszawa: 18-44, 255-256.

Burnell A. M. & Stock S. P., 2000, Heterorhabdits, Steinernema and their bacterial symbionts – lethal pathogens of insect, Nematology 2: 31-42. DOI: 10.1163/156854100508872

Campos-Herrera R., Escuer M., Robertson L. & Gutiérrez C., 2006, Morphological and Ecological Characterization of Steinernema feltiae (Rhabditida: Steinernematidae) Rioja Strain Isolated from Bibio hortulanus (Diptera: Bibionidae) in Spain, The Journal of Nematology 38(1): 68-75.

Dreistadt S. H., 2004, Pests of Landscape Trees and Shrubs: An Integrated Pest Management Guide, Agriculture & Natural Resources, Oakland, California, USA.

Fan X. & Hominick W. M., 1991, Efficiency of Galleria (Wax moth) baiting technique for recovering infective stages of entomopathogenic rhabdatids (Steinernematidae and Heterorhabtidae) from sand and soil, Review of Nematology 14: 381-387.

Grant J. A. & Villani M. G., 2003, Soil moisture effects on entomopathogenic nematodes, Environmental Entomology 32(1): 80-87. DOI 10.1603/0046-225X-32.1.80

Kaya H. K., 1990, Soil Ecology, [in:] R. Gaugler, H. K. Kaya (eds) Entomopathogenic Nematodes in Biological Control, Boca Raton, FL, CRC Press: 93-115.

Koppenhofer A. M. & Fuzy E. M., 2006, Effect of soil type on infectivity and persistence of the entomopathogenic nematodes Steinernema scarabaei, Steinernema glaseri, Heterorhabditis zealandica and Heterorhabditis bacteriophora, Journal of Invertebrate Pathology 92(1):11-22. DOI 10.1016/j.jip.2006.02.003

Lacey L. A. & Georgis R., 2012, Entomopathogenic Nematodes for Control of Insect Pests Above and Below Ground with Comments on Commercial Production, Journal of Nematology 44(2): 218-225.

Lacey L. A., Arthurs S. P., Unruh T. R., Headrick H. & Fritts Jr. R., 2006, Entomopathogenic nematodes for control of codling moth (Lepidoptera: Tortricidae) in apple and pear orchards: Effect of nematode species and seasonal temperatures, adjuvants, application equipment, and post-application irrigation, Biological Control 37: 214-223. DOI 10.1016/j.biocontrol.2005.09.015

Marko-Worłowska M. & Chrzan A., 2010, Wpływ antropopresji na entomofaunę łąki i lasu w Parku Krajobrazowym w Krakowie Zakrzówku [The impact of anthropopressure on meadow and forest entomofauna in the Landscape Park of Kraków Zakrzówek], Proceedings of ECOpole 4(1): 161-165.

Mason J. M. & Hominick W. M., 1995, The effect of temperature on infection, development, and reproduction of Heterorhabditids, Journal of Helminthology 69(4): 337-345. DOI 10.1017/S0022149X00014929

Nguyen K. B. & Hunt D. J., 2007, Entomopathogenic Nematodes: Systematics, Phylogeny and Bacterial Symbionts, Brill. Leiden-Boston 5: 63-369. DOI: 10.1163/ej.9789004152939.i-816

Shapiro-Ilan D. I., Cottrell T. E., Mizell III R. F., Horton D. L. & Davis J., 2009, A novel approach to biological control with entomopathogenic nematodes: Prophylactic control of the peachtree borer, Synanthedon exitiosa, Biological Control 48: 259-263. DOI 10.1016/j.biocontrol.2008.10.008

Strong D. R., Kaya H. K., Whipple A. V., Child A. L., Kraig S., Bondonno M., Dyer K. & Maron J. L., 1996, Entomopathogenic nematodes: Natural enemies of root-feeding caterpillars on bush lupine, Oecologia 108(1): 167-173. DOI 10.1007/BF00333228

Szabla K. & Pabian R., 2003, Szkółkarstwo kontenerowe. Nowe technologie i techniki w szkółkarstwie leśnym [Container plant nursery. New Technologies and techniques in forest nurseries], Centrum Informacyjne Lasów Państwowych, Warszawa.

Yadav A. K. & Lalramliana, 2012, Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India, Journal of Parasitic Diseases 36(1): 94-98. DOI 10.1007/s12639-011-0076-x

Partnerzy platformy czasopism