Waste water treatment by exoelectrogenic bacteria isolated from technogenically transformed lands

Svitlana Нnatush, Olha Maslovska, Tetyana Sehin, Oresta Vasyliv, Myroslav Kovalchuk, Myroslav Malovanyy

DOI: http://dx.doi.org/10.12775/28168

Abstract


The capacity of sulfur-reducing bacteria Desulfuromonas acetoxidans IMV B-7384, Geobacter sp. CB35 and Desulfuromusa sp. CB30 and green photosynthesizing bacteria Chlorobium limicola IMV K-8 for exoelectrogenesis was investigated during their growth in wastewater of industrial and municipal origin. The strains of exoelectrogens, which are characterized by resistance to heavy metal ions, were isolated from the man-made Yavorivske lake located in the Lviv Oblast in Ukraine (D. acetoxidans IMV-7384, Ch. limicola IMV K-8) and mine waste heaps of the Chervonohrad mining industry region (Geobacter sp. CB 30 and Desulfuromusa sp. CB 35). Bacteria D. acetoxidans IMВ B-7384 proved to be the most effective exoelectrogens. The power density of a microbial fuel cell (MFC) with the application of D. acetoxidans IMV B-7384 and the infiltrate of the Lviv solid waste landfill was 2.0 ± 0.05 W/m2 and the reduction of chemical oxygen demand of wastewater was 99%. The new approach to improving the MFC performance was investigated. It includes a combination of phototrophic microorganisms Ch. limicola and heterotrophic microorganisms, which reduce the content of nitrates, nitrites, ammonia, sulfates, sulfites, hydrogen sulfide, while simultaneously generating electric current.

Keywords


wastewater; bioremediation; microbial fuel cell; bacteria-exoelectrogens

Full Text:

PDF

References


Badalamenti J., 2013, Coupling dark metabolism to electricity generation using photosynthetic cocultures. Biotechnology and Bioengineering 111(2): 223-31.

Chaturvedi V. & Verma P., 2016, Microbial fuel cell: a green approach for the utilization of waste for the generation of bioelectricity. Bioresources and Bioprocessing 3: 38. (https://doi.org/10.1186/s40643-016-0116-6).

Do M., Ngo H., Guo W., Liu Y., Chang S.W., Nguyen D.D., Nghiem L.D. & Ni B.J., 2018, Challenges in the application of microbial fuel cells to wastewater treatment and energy production: a mini review. Science of the Total Environment 639: 910-920.

Dowdy F., Kawakita R., Lange M. & Simmons C., 2017, Meta-analysis of microbial fuel cells using waste substrates. Applied Biochemistry and Biotechnology 185(1): 221-232.

Diakiv S., Hnatush S. & Moroz O., 2016, Sulfur reducing bacteria from coal pits waste heaps of Chervonograd minig region. Studia Biologica 10(2): 63-76.

Gajda I., Greenman J. & Ieropoulos I., 2018, Review article recent advancements in real-world microbial fuel cell applications. Current Opinion in Electrochemistry 11: 78-83.

Gudz S., Hnatush S., Moroz O. Peretiatko, T. & Vasyliv, O., 2013, Certificate of deposition of strain of bacteria Desulfuromonas acetoxidans Ya-2006 in the Depository of Institute of Microbiology and Virology, NAS of Ukraine on granting registration number IMV B-7384 of 10 April 2013.

He L., Du P., Chen Y., Lu H., Cheng X., Chang B. & Wang Z., 2017, Advances in microbial fuel cells for wastewater treatment. Renewable and Sustainable Energy Reviews 71: 388-403.

ISO 17381:2003 Water quality – Selection and application of ready-to-use test kit methods in water analysis.

Kharayat Y., 2012, Distillery wastewater: bioremediation approaches. Journal of Integrative Environmental Sciences 9(2): 69-91.

Logan B., 2009, Exoelectrogenic bacteria that power microbial fuel cells. Nature Reviews Microbiology 7: 375–381.

Maslovscka O., 2017, Antioxidant defense and fatty acid composition of Desulfuromonas acetoxidans IMV B-7384 under the influence of ferric (III) citrate. Ph.D. Thesis, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv.

Methods for chemical analysis of water and wastes, 1983, U.S. Environmental Protection Agency, Office of Research and Development, Washington, DC 20460, 491 pp.

Moroz O. & Rusyn I., 2012, Usage of nitrogen сompounds by sulfur cycle bacteria of Yavorivske Lake. Microbiology and Biotechnology 2: 96-108.

Qi X., Ren Y., Liang P. & Wang X., 2018, New insights in photosynthetic microbial fuel cell using anoxygenic phototrophic bacteria. Bioresource Technology 258: 310–317.

Patent of Ukraine, 2011, MPK N01M8/00; N01M 8/16; N01M 8/22. Consortium of Chlorobium limicola Ya-2002 and Pseudomonas sp. – producer of glycogen / Gorishnyi M.B., Gudz S.P., Moroz O. M., Hnatush S. O., Levytska O.; applicant and owner is Ivan Franko National University of Lviv. − No. 63723 ; stated on 25.11.2011.

Protocol for the Sampling and Analysis Of Industrial/Municipal Wastewater. Version: 2.0, 2016, Ontario Ministry of the Environment and Climate Change, Laboratory Services Branch, Ontario, 195 pp.

Santoro C., Arbizzani C. & Erable B., 2017, Microbial fuel cells: From fundamentals to applications. A review. Journal of Power Sources 356: 225–244.

Sereda A., 2018, Two-stage purification of landfill infiltrates in aerobic lagoons and municipal wastewater treatment facilities. Ph.D. Thesis, Lviv Polytechnic National University Ministry of Education and Science of Ukraine, Lviv.

Shrivastava S. & Bundela H., 2013, Power generation through double chamber MFC operation by slurry mixed with different substrates. International Journal Engineering Trends and Technology 4: 4201-4205.

Tharali A., Sain N. & Osborne W., 2016, Microbial fuel cells in bioelectricity production. Frontiers in Life Science 9(4): 252-266.

Vasyliv O., 2013, The influence of 3d-type transition metals on physiological and biochemical properties of Desulfuromonas acetoxidans bacteria. Ph.D. Thesis, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv.

Wang J.-P. & Dong Q.-H., 2018, Analysis and discussion on the calculation formula of the classical monitoring method of the permanganates index (IMn). Journal of Education and Practice 9(22): 1-4.

Włodarczyk B. & Włodarczyk P., 2017, Microbial fuel cell with Cu-B cathode powering with wastewater from yeast production. Journal of Ecological Engineering 18(4): 224-230.

https://www.genome.jp/kegg-bin/show_pathway?pvi00910.




Partnerzy platformy czasopism