Microorganisms from harsh and extreme environments: a collection of living strains at ACUF (Naples, Italy)

Luigi D’Elia, Angelo Del Mondo, Mariano Santoro, Antonino De Natale, Gabriele Pinto, Antonino Pollio

DOI: http://dx.doi.org/10.12775/EQ.2018.023

Abstract


The Algal Collection at the University Federico II (ACUF) is a bioresource center where over 800 live microalgal strains are maintained, mainly belonging to Cyanobacteria, Chlorophyta, Rhodophyta, and Bacillariophyceae. The extremophilic algae maintained at ACUF include thermo-acidophilic and acidotolerant strains, mostly belonging to the Cyanidiophyceae isolated from European and extra-European sites, and also terrestrial isolates from bare rocks and monuments. The main target of the ACUF Center is the study and preservation of the diversity of extremophylic microalgae. This collection is used as a resource for studies about biochemical and evolutionary strategies as well as mechanisms involved in cell functioning under harsh environmental conditions. These organisms can be also useful sources for the production of chemical compounds or other biological products with potential biotechnological applications.


Keywords


Culture collection; extreme environments; microalgae; biodiversity; biotechnology

Full Text:

PDF

References


Albertano P., Pinto G., Pollio A., 1994, Ecophysiology and ultrastructure of an acidophilic species of Ochromonas (Chrysophyceae, Ochromonadales), Archiv Für Protistenkunde 144 : 75-82.

Albertano P., Pinto G., Pollio A., Taddei R., 1991, Physiological, biochemical, and ultrastructural characters of some strains of Viridiella fridericiana (Chlorophyta, Chlorococcales), Archiv Für Protistenkunde 139 : 117-23.

Albertano P., Pinto G., Pollio A., Taddei R., 1990, Morphology, ultrastructure and ecology of an acidophilic alga, Pseudococcomyxa simplex (Mainx) Fott (Chlorococcales), Algological Studies/Archiv Für Hydrobiologie S59 : 81-95.

Albertano P., Taddei R., 1984, Chlorella protothecoides Krüger var. acidicola, a new variety from very low pH environments, Algological Studies/Archiv Für Hydrobiologie 37 : 401-408.

Allen M. B., 1959, Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte, Archiv fur Mikrobiologie 32 : 270-277.

Aminot A., Rey F., 2000, Standard procedure for the determination of chlorophyll a by spectroscopic methods international council for the exploration of the sea, Conseil International Pour l’Exploration de La Mer, Copenhagen, Denmark.

Bischoff H. W., Bold H. C., 1963, Some soil algae from enchanted rock and related algal species, Phycological Studies IV, Austin Texas Publication 6318 : 1-95.

Bold H. C., 1949, The morphology of Chlamydomonas chlamydogama, sp. nov. Bulletin of the Torrey Botanical Club 76(2) : 101-108.

Borderie F., Denis M., Barani A., Alaoui-Sossé B., Aleya L., 2016, Microbial composition and ecological features of phototrophic biofilms proliferating in the Moidons Caves (France): investigation at the single-cell level, Environmental Science and Pollution Research 23(12) : 12039-12049.

Caisová L., Marin B., Melkonian M., 2013, A consensus secondary structure of ITS2 in the chlorophyta identified by phylogenetic reconstruction, Protist 164 (4) : 482-96.

Carfagna S., Landi V., Coraggio F., Salbitani G., Vona V., Pinto G., Pollio A., Ciniglia C., 2018, Different characteristics of C-phycocyanin (C-PC) in two strains of the extremophilic Galdieria phlegrea, Algal Research 31: 406-12.

Ciniglia C., Yang E. C., Pollio A., Pinto G., Iovinella M., Vitale L., Yoon H. S., 2014, Cyanidiophyceae in Iceland: plastid rbcL gene elucidates origin and dispersal of extremophilic Galdieria sulphuraria and G. maxima (Galdieriaceae, Rhodophyta). Phycologia 53(6) : 542-51.

Ciniglia C., Cennamo P., De Stefano M., Pinto G., Caputo P., Pollio A., 2007, Pinnularia obscura Krasske (Bacillariophyceae, Bacillariophyta) from acidic environments: characterization and comparison with other acid-tolerant Pinnularia species, Fundamental and Applied Limnology / Archiv Für Hydrobiologie 170(1) : 29-47.

Ciniglia C., Cascone C., Lo Giudice R., Pinto G., Pollio A., 2005, Application of methods for assessing the geno- and cytotoxicity of Triclosan to C. ehrenbergi, Journal of Hazardous Materials 122(3) : 227-32.

Ciniglia C., Yoon H. S., Pollio A., Pinto G., Bhattacharya D., 2004, Hidden biodiversity of the extremophilic Cyanidiales red algae, Molecular Ecology 13(7) : 1827-38.

Cozzolino S., Pinto G., Pollio A., De Luca P., 1999, Lack of trnL intron in the thermoacidophilic red alga Galdieria sulphuraria (Galdieri) Merola, Plant Biosystems 133(3) : 303-5.

De Luca P., Musacchio A., Taddei R., 1981, Acidophilic algae from the fumeroles of Mount Lawu (Java), locus classicus of Cyanidium caldarium Geitler, Giornale Botanico Italiano 115: 1 -9.

De Luca P., Moretti A., Taddei R., 1976-77, Presenza di Cyanidioschyzon merolae De Luca, Taddei, Varano in ambienti acidi extraeuropei (U.S.A. e Indonesia), Delpinoa n.s. 18-19 : 69 -76.

De Luca P., Taddei R., Varano L., 1978, Cyanidioschyzon merolae: a new alga of thermal acidic environments, Webbia 33(1) : 37-44.

De Luca P., Moretti A., Taddei R., 1972-73, Nuove stazioni di Cyanidium caldarium nell’Italia meridionale ed in Sicilia, Delpinoa n.s. 14-15 : 49-60.

Del Mondo A., Pinto G., De Natale A., Pollio A., 2017, In vitro colonization experiments for the assestmentas mycelial growth on a tuff substratum by a Fusarium solani strain isolated from the Oplontis (Naples, Italy) archeological site, International Journal of Conservations Science 8(4) : 651-62.

Doyle J. J., Doyle J. L., 1990, Isolation of plant DNA from fresh tissue, Focus 12 : 13-15.

Dubelaar G. B. J., Jonker R. R., Jonker R. R., 2000, Flow cytometry as a tool for the study of phytoplankton, Scientia Marina 64(2) : 135-56.

Fama P., Wysor B., Kooistra W. H. C. F., Zuccarello G. C., 2002, Molecular phylogeny of the genus Caulerpa (Caulerpales, Chlorophyta) inferred from chloplast tufA gene, Journal of Phycology 38(5) : 1040-50.

Ferrari B., Paxéus N., Lo Giudice R., Pollio A., Garric J., 2003, Ecotoxicological impact of pharmaceuticals found in treated wastewaters: study of carbamazepine, clofibric acid, and diclofenac. Ecotoxicology and Environmental Safety 55(3) : 359-70.

Fuíková K., Flechtner V. R., Lewis L. A., 2013, Revision of the genus Bracteacoccus Tereg (Chlorophyceae, Chlorophyta) based on a phylogenetic approach, Nova Hedwigia 96(1-2) : 15-59.

Gambardella R., Moretti A., Musacchio A., 1979-80, Autoecologia ed ultrastruttura di alghe termoacidofile del nord e centro America, Delpinoa n.s. 21-22 : 35-45.

Gifuni I., Olivieri G., Pollio A., Franco T. T., Marzocchella A., 2017, Autotrophic starch production by Chlamydomonas species. Journal of Applied Phycology 29(1) : 105-14.

Gifuni I., Olivieri G., Pollio A., Marzocchella A., 2018, Identification of an industrial microalgal strain for starch production in biorefinery context: the effect of nitrogen and carbon concentration on starch accumulation. New Biotechnology 41 : 46-54.

Graziani G., Schiavo S., Nicolai M. A., Buono S., Fogliano V., Pinto G., Pollio A., 2013, Microalgae as human food: chemical and nutritional characteristics of the thermo-acidophilic microalga Galdieria sulphuraria, Food Function 4(1) : 144-52.

Hall J. D., Fuωíková K., Lo C., Lewis A L., Karol K. G., 2010, An assessment of proposed DNA barcodes in freshwater green algae, Cryptogamie, Algologie 31(4) : 529-55.

Hirose H., 1958, Rearrangement of the systematic position of a thermal alga, Cyanidium caldarium, Botanical Magazine, Tokyo 71 : 347 -352.

Holzinger A., Karsten U. 2013, Desiccation stress and tolerance in green algae: consequences for ultrastructure, physiological and molecular mechanisms, Frontiers in Plant Science 4 : 327.

Hyka P., Lickova S., Přibyl P., Melzoch K., Kovar K., 2013, Flow Cytometry for the Development of Biotechnological Processes with Microalgae, Biotechnology Advances 31 : 2-16.

Izabela R., Katarzyna G., Borys K., Agnieszka M., Ignacy G.W., Maciej G., 2008, 3D chloroplast structure. In: Allen J.F., Gantt E., Golbeck J.H., Osmond B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht.

Janda J. M., Abbott S. L., 2007, 16S rRNA Gene Sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls, Journal of Clinical Microbiology 45 : 2761-64.

Manen J. F., Jacques F., 2002, The cpcB -cpcA Locus as a tool for the genetic characterization of the genus Arthrospira (Cyanobacteria): evidence for horizontal transfer, International Journal of Systematic and Evolutionary Microbiology 52 : 861-67.

Marasco A., Nocerino S., Pinto G., Pollio A., Trojsi G., De Natale A., 2016, Weathering of a Roman mosaic—A biological and quantitative study on in vitro colonization of calcareous tesserae by phototrophic microorganisms, Plos One 11.

Meneghini G., 1839, Nuova specie di alga descritta dal sig. Dott. Giuseppe Meneghini di Padova, Nuovo Giornale De’ Letterati 39 : 67-68.

Merola A., Castaldo R., De Luca P., Gambardella R., Musacchio A., Taddei R., 1981, Revision of Cyanidium caldarium. Three species of acidophilic algae, Giornale Botanico Italiano 115 : 189-95.

Müller K. M., Oliveira M. C., Sheath R. G., Bhattacharya D., 2001, Ribosomal DNA phylogeny of the Bangiophycidae (Rhodophyta) and the origin of secondary plastids, American Journal of Botany 88 : 1390-400.

Olivieri G., Marzocchella A., Salatino P., Andreozzi R., Pinto G., Pollio A., 2010, Bio -oil production by Stichococcus strains in laboratory scale photobioreactors, Journal of Biotechnology 150 : 18.

Organization for Economic Co -operation and development, 1993, OECD guidelines for testing of chemicals 201 -alga, growth inhibition test. OECD guidelines for the testing of chemicals, vol. 1, Paris.

Pinto G., Pollio A., Previtera L., Stanzione M., Temussi F., 2003, Removal of low molecular weight phenols from olive oil mill wastewater using microalgae, Biotechnology Letters 25 : 1657-59.

Pinto G., Taddei R., 1976-77, Le alghe delle acque e dei suoli acidi italiani, Delpinoa n.s. 18-19: 77-106.

Pollio A., Aliotta G., Pinto G., Paterno M., Bevilacqua A., 1997, Ecophysiological characters and biochemical composition of Stichococcus bacillaris Naegeli strains from low pH environments, Algological Studies/Archiv Für Hydrobiologie S84 : 129-43.

Pollio A., Cennamo P., Ciniglia C., De Stefano M., Pinto G., Huss V. A., 2005, Chlamydomonas pitschmannii Ettl, a little known species from thermoacidic environments, Protist 156 : 287-302.

Rampelotto P., 2013, Extremophiles and extreme environments, Life 3 : 482-85.

Rippka R., Deruelles J., Waterbury J., Herdman M., Stanier R., 1979, Generic assignments, strain histories and properties of pure cultures of cyanobacteria, Journal of General Microbiology, 111 : 1-61.

Rippka R., Waterbury J. B., Stanier R. Y,. 1981, Isolation and purification of cyanobacteria: some general principles, In: Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. (eds) The Prokaryotes. Springer, Berlin, Heidelberg, 212-20.

Ruiz G.J., Olivieri G., de Vree J., Bosma R., Willems P., Reith H., Eppink M., Kleinegris D., Wijffels R., Barbosa M.J., 2016, Towards industrial products from microalgae, Energy and Environmental Science, 9(10):3036-3043.

Trask B. J., van den Engh G. J., Elgershuizen J. H., 1982, analysis of phytoplankton by flow cytometry, Cytometry 2(4) : 258-64.

U.S. EPA, 1989, Algal (Selenastrum capricornutum) growth test. Short - term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms, Environmental Monitoring Systems Laboratory, Environmental Protection Agency, Cincinnati, Ohio.

Vieira H.H., Bagatini I. L., Guinart C. M., Vieira H. A. A., 2016, tufA Gene as molecular marker for freshwater Chlorophyceae, Algale 31 : 155-65.

Walker J. J., Pace N. R., 2007, Endolithic microbial ecosystems, Annual Review of Microbiology 61 : 331-47.

Wang X. C., Chang L., Liang H., Bengtsson-Palme J., Chen H., Zhang J. H., Cai D., Li J. Q., 2015, ITS1 a DNA barcode better than ITS2 in eukaryotes? Molecular Ecology Resources 15(3) : 573 -86.

Wellburn A. R., 1994, The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution, Journal of Plant Physiology 144 : 307-13.

WWAP (United Nations World Water Assessment Programme), 2015, The United Nations World Water Development Report 2015: Water for a sustainable world. Paris, UNESCO.

Yoon H. S., Hackett J. D., Pinto G., Bhattacharya D., 2002 Nonlinear partial differential equations and applications: from the cover: the single, ancient origin of chromist plastids, Proceedings of the National Academy of Sciences 99 : 15507-12.

Yoon H. S., Ciniglia C., Wu M., Comeron J. M., Pinto G., Pollio A., Bhattacharya D., 2006, Establishment of endolithic populations of extremophilic Cyanidiales (Rhodophyta), BMC Evolutionary Biology 6 : 78.

Zehr J. P., Mellon M. T., Hiorns W. D., 1997, Phylogeny of cyanobacterial nifH genes: evolutionary implications and potential applications to natural assemblages, Microbiology 143 : 1443-50.

Zhu L., 2015, Biorefinery as a promising approach to promote microalgae industry: an innovative framework, Renewable and Sustainable Energy Reviews 41: 1376-84.

Zou S., Fei C., Wang C., Gao Z., Bao Y., He M., Wang C., 2016, How DNA barcoding can be more effective in microalgae identification: a case of cryptic diversity revelation in Scenedesmus (Chlorophyceae), Scientific Reports 6 : 36822.




Partnerzy platformy czasopism