Association of bovine CXCL8 polymorphism with clinical mastitis and fertility trait in Polish HF cattle
DOI:
https://doi.org/10.12775/TRVS.2020.006Keywords
trait-associated, breeding values, interleukin-8, CXCL8, somatic cell count, clinical mastitis, fertility trait, Polish HFAbstract
Background: The aim of this study was to investigate the relationship between CXCL8 (CXCL8c.105A>G and CXCL8c.210C>T) SNP polymorphism and the clinical mastitis and production trait in Polish HF cattle.
Methods: The trait-associated CXCL8 SNPs polymorphism study was carried out in the Polish HF bulls (n=151) by PCR-RFLP methods as described in the previous issue of this journal. The phenotypic records (assessment year 2017) of clinical mastitis, functional and fertility trait were collected from the Research Institute of Animal Production, Balice, Poland (http://www.izookrakow.pl).
Results: Overall, the trait-associated study revealed no relationship between the CXCL8c.210C>T SNP polymorphism and the breeding value of selected clinical mastitis and fertility trait of breeding bulls. However, TT genotypes showed low levels of significance of differences for the breeding values of somatic cells count (p = 0.27) and stillborn calving of heifers (p=0.27). Similarly, the trait-associated study showed no correlation between the CXCL8c.105A>G polymorphism and the breeding value of selected clinical mastitis and fertility trait of breeding bulls. However, AA genotypes showed low levels of significance of differences for the somatic cell count (p = 0.12), ease calving of heifers (p = 0.14), the difficulty calving of heifers (p = 0.17), live-born calves of heifers (p = 0.21), and AG genotypes showed the low levels of significance of differences for the stillborn calving in heifers (p = 0.21).
Conclusions: Study concludes that trait-associated studies of CXCL8 polymorphism did not identify highly significant effects on clinical mastitis and fertility trait in the investigated breeding bulls of Polish HF cattle.
References
Kumar N., Manimaran A., Kumaresan A., Jeyakumar S., Sreela L., Mooventhan P., et al. Mastitis effects on reproductive performance in dairy cattle: a review. Trop Anim Health Prod. 2017;49:663–73.
Peters M.D., Silveira I.D., Fischer V. Impact of subclinical and clinical mastitis on sensitivity to pain of dairy cows. Animal, 2015;9:2024–8.
Heikkila A.M., Nousiainen J.I., Pyorala S. Costs of clinical mastitis with special reference to premature culling. J Dairy Sci. 2012;95:139–50.
Ahmadzadeh A., Frago F., Shafii B., Dalton J.C., Price W.J., McGuire M.A. Effect of clinical mastitis and other diseases on reproductive performance of Holstein cows. Anim Reprod Sci. 2009;112:273–82.
Kirsanova E., Heringstad B., Lewandowska-Sabat A., Olsaker I. Identification of candidate genes affecting chronic subclinical mastitis in Norwegian Red cattle: combining genome-wide association study, topologically associated domains and pathway enrichment analysis. Anim Genet. 2020;51:22-31.
Kirsanova E., Boysen P., Johansen G.M., Heringstad B., Lewandowska-Sabat A., Olsaker I. Expression analysis of candidate genes for chronic subclinical mastitis in Norwegian Red cattle. J Dairy Sci. 2020;103:9142-9149.
Christopher D. Russell, Stephanie Widdison, James A. Leigh, Tracey J. Coffey Identification of single nucleotide polymorphisms in the bovine Toll-like receptor 1 gene and association with health traits in cattle, Vet Res. 2012;43:17.
Farhat K., Sauter K.S., Brcic M., Frey J., Ulmer A.J., Jungi T.W. The response of HEK293 cells transfected with bovine TLR2 to established pathogen-associated molecular patterns and to bacteria causing mastitis in cattle. Vet Immunol Immunopathol. 2008;125:326-36.
Lee J.W., Bannerman D.D., Paape M.J., Huang M.K., Zhao X Characterization of cytokine expression in milk somatic cells during intramammary infections with Escherichia coli or Staphylococcus aureus by real-time PCR. Vet Res. 2006;37:219-29.
Czarnik U., Kreft K., Sachajko M., Herudzińska M., Żukowski K., Pareek C.S. Identification of CXCL8c.105A>G and CXCL8c.210C>T polymorphism in Polish HF cattle. Transl. Res Vet Sci. 2020;3:32-39.
Bhattarai D., Chen X., Ur Rehman Z., Hao X., Ullah F., Dad R., Talpur H.S., Kadariya I., Cui L., Fan M., Zhang S. Association of MAP4K4 gene single nucleotide polymorphism with mastitis and milk traits in Chinese Holstein cattle. J Dairy Res. 2017;84:76-79.
Pant S.D., Schenkel F.S., Leyva-Baca I., Sharma B.S., Karrow N.A. Identification of single nucleotide polymorphisms in bovine CARD15 and their associations with health and production traits in Canadian Holsteins. BMC Genomics. 2007;8:421.
Sharma B.S., Leyva I., Schenkel F., Karrow N.A. Association of toll-like receptor 4 polymorphisms with somatic cell score and lactation persistency in Holstein bulls. J Dairy Sci. 2006;89:3626-35.
Usman T., Wang Y., Liu C., Wang X., Zhang Y., Yu Y. Association study of single nucleotide polymorphisms in JAK2 and STAT5B genes and their differential mRNA expression with mastitis susceptibility in Chinese Holstein cattle. Anim Genet. 2015;46:371-80.
Alain K., Karrow N.A., Thibault C., St-Pierre J., Lessard M., Bissonnette N. Osteopontin: an early innate immune marker of Escherichia coli mastitis harbors genetic polymorphisms with possible links with resistance to mastitis. BMC Genomics. 2009;10:444.
Russell C.D., Widdison S., Leigh J.A., Coffey T.J. Identification of single nucleotide polymorphisms in the bovine Toll-like receptor 1 gene and association with health traits in cattle. Vet Res. 2012;43:17.
Downloads
Published
How to Cite
Issue
Section
License
Title, logo and layout of TR in VS are reserved trademarks of TR in VR.
Stats
Number of views and downloads: 352
Number of citations: 0
