https://apcz.umk.pl/TRVS/issue/feed Translational Research in Veterinary Science 2021-01-25T10:50:09+01:00 Chandra S. Pareek pareekcs@umk.pl Open Journal Systems <p>Translational Research in Veterinary Science is an open access, peer reviewed journal that publishes all aspects of -omics research in the field of veterinary science. Translational Research in veterinary science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of multi- and inter- disciplinary veterinary and biomedical research.</p><p>Index Copernicus Value (ICV) 2019:<br /> ICV 2019 = 72.73</p> https://apcz.umk.pl/TRVS/article/view/32900 Preface 2021-01-25T10:47:45+01:00 Chandra Shekhar Pareek pareekcs@umk.pl <p>Third volume and the second issue (Year 2020) of the scholarly open-access journal Translational Research in Veterinary Science (TRVS), Nicolaus Copernicus University (NCU) publishers is publishing four research papers devoted in the field of veterinary science. In this published issue of TRVS, three manuscripts based on the original research works and a case report. The first paper of <strong>Czarnik </strong><strong><em>et al. </em></strong>(2020) published the original research paper based on the trait-associated study conducted on Polish HF cattle to find out the genetic association between bovine CXCL8 gene polymorphism and clinical mastitis and fertility trait; the second paper of <strong>Kwiatkowska </strong><strong><em>et al</em></strong><em>. </em>(2020) published the case report study on cat suffering from gastrointestinal lymphoma to examine the association of toxic encephalopathy with low dose metronidazole therapy; the third paper of <strong>Yemets </strong>(2020) published the original research paper based on the animal nutrition studies describing the dietary effects of drone larves homogenate on the homeostatic constants and the reproductive capacity of Large White gilts in Ukraine; and lastly the <strong>Bak </strong><strong><em>et al. </em></strong>(2020) published the original research paper based on the use of algorithms for multiple DNA sequence alignment across livestock species. During this difficult period of COVID-19 pandemic year, I would like to thank all authors for their contribution to this issue. Thanks are also extended to the editorial board members, manuscripts reviewers, TRVS editorial technical staff working at NCU publisher office (<strong>Mirosława Buczyńska, Elżbieta Kossarzecka, Grzegorz Kopcewicz, and Dariusz Żulewski</strong>), who supported at every stage of the project. Most importantly, this volume of TRVS is greatly acknowledged the supported of NCU funds from newly elect JM Rector <strong>Prof. dr hab. Andrzej Sokala</strong>.</p> 2021-01-20T00:00:00+01:00 Copyright (c) 2021 Translational Research in Veterinary Science https://apcz.umk.pl/TRVS/article/view/TRVS.2020.006 Association of bovine CXCL8 polymorphism with clinical mastitis and fertility trait in Polish HF cattle 2021-01-24T02:41:46+01:00 Urszula Czarnik czar@uwm.edu.pl Marta Barcewicz barcewiczmarta@gmail.com Mateusz Sachajko mateuszsachajko@gmail.com magdalena Herudzińska mherudzinska@umk.pl Kacper Żukowski kzukowski@umk.pl Chandra Shekhar Pareek pareekcs@umk.pl <p><strong>Background: </strong>The aim of this study was to investigate the relationship between <em>CXCL8</em> (<em>CXCL8</em>c.105A&gt;G and <em>CXCL8</em>c.210C&gt;T) SNP polymorphism and the clinical mastitis and production trait in Polish HF cattle.</p><p><strong>Methods: </strong>The trait-associated <em>CXCL8</em> SNPs polymorphism study was carried out in the Polish HF bulls (n=151) by PCR-RFLP methods as described in the previous issue of this journal. The phenotypic records (assessment year 2017) of clinical mastitis, functional and fertility trait were collected from the Research Institute of Animal Production, Balice, Poland (<a href="http://www.izookrakow.pl/">http://www.izookrakow.pl</a>).</p><p><strong>Results: </strong>Overall,<strong> </strong>the trait-associated study revealed no relationship between the <em>CXCL8</em>c.210C&gt;T SNP polymorphism and the breeding value of selected clinical mastitis and fertility trait of breeding bulls. However, TT genotypes showed low levels of significance of differences for the breeding values of somatic cells count (p = 0.27) and stillborn calving of heifers (p=0.27). Similarly, the trait-associated study showed no correlation between the <em>CXCL8</em>c.105A&gt;G polymorphism and the breeding value of selected clinical mastitis and fertility trait of breeding bulls. However, AA genotypes showed low levels of significance of differences for the somatic cell count (p = 0.12), ease calving of heifers (p = 0.14), the difficulty calving of heifers (p = 0.17), live-born calves of heifers (p = 0.21), and AG genotypes showed the low levels of significance of differences for the stillborn calving in heifers (p = 0.21).</p><p><strong>Conclusions:</strong> Study concludes that trait-associated studies of <em>CXCL8</em> polymorphism did not identify highly significant effects on clinical mastitis and fertility trait in the investigated breeding bulls of Polish HF cattle.</p> 2021-01-24T00:00:00+01:00 Copyright (c) 2021 Translational Research in Veterinary Science https://apcz.umk.pl/TRVS/article/view/TRVS.2020.007 Toxic encephalopathy associated with low dose metronidazole therapy in a cat suffering from gastrointestinal lymphoma: A case report 2021-01-25T10:48:52+01:00 Miłosława Kwiatkowska kwiatkowska.miloslawa@umk.pl Urszula Pasławska urszula.paslawska@umk.pl Jedrzej M. Jaśkowski jmjaskowski@umk.pl <p>This case report describes an episode of acute encephalopathy in a cat treated with metronidazole due to chronic gastrointestinal disease (giardiasis). Metronidazole had been administered at a dose of 28 mg/ kg/day for 7 days. Cat showed signs of head pressing, reduced consciousness, ataxia, seizures, lack of menace response and progressive weakness. Complete blood cell count revealed haemolytic anaemia, serum biochemistry, coagulation profile, blood pressure measurement, urinalysis, were unremarkable. Metronidazole administration was immediately discontinued; supportive care consisted of fluid therapy, blood transfusion was also performed. The neurological status of the patient improved rapidly within 72 h. Anaemia improved in next 7 days, however transfusion and steroid treatment was necessary. Two weeks post intoxication abdominal ultrasound revealed abnormal hepatic pattern, enlarged mesenteric lymph nodes and lack of normal intestinal layering. A biopsy had been taken during explorative laparotomy and histopathologic examination revealed gastrointestinal and hepatic lymphoma. The aim of this case report is to present that cats suffering from multiple conditions may show metronidazole intoxication even when recommended dosage and treatment length is used. We cannot exclude that metronidazole was responsible for autoimmune haemolytic anaemia.</p> 2021-01-24T00:00:00+01:00 Copyright (c) 2021 Translational Research in Veterinary Science https://apcz.umk.pl/TRVS/article/view/TRVS.2020.008 Dietary effects of drone larves homogenate on the homeostatic constants and the reproductive capacity of Large White gilts 2021-01-25T10:49:30+01:00 Yaroslav Mykolaiovych Yemets yiemets21@gmail.com <strong>Background:</strong> With modern requirements for housing agricultural animals particularly pigs, the manifestation of normal physiological functions significantly changes, especially reproductive one, which is manifested in an insufficiently expressed sexual cycle, reduced resistance due to technological stress. There is a need to develop effective reproductive biotechnologies in pig using organic stimulants of entomological origin, among which a significant place is given to the homogenate of drone larvae (HDL).The aim of the study was to determine the dietary effects of HDL on the formation of prooxidant-antioxidant homeostasis in Large White (LW) gilts,as well as to optimize reproductive function during experimental feedingof HDL.<br /><br /><strong>Methods:</strong> In the study, gilts representing Large White breed were investigated. A total of 20 gilts representing two groups. The first feeding group (n=10) of LW gilts was fed with the control normal diet, and the second feeding group (n=10) of LW gilts wasfed with the supplement diet i.e., the biological additive of homogenate drone larvae in quantity of 0.5g daily. To assess prooxidant-antioxidant homeostasis in pigs, blood samples were taken from the anterior vena cava into the diestrus phase i.e., 11 days from the beginning of the second cycle after the determination of the immobility reflex and the estrus phase i.e., 24 days after the start of estrus after the determination of the immobility reflex. Intracervical artificial insemination of gilts was performed during 24 and 36 hours after the determination of the immobility reflex, and depending on the manifestation of signs of estrus.<br /><br /><strong>Results:</strong>Study found an intensification of peroxidation processes during the period of puberty in LW gilts , which wasmanifested in an increase in the content of diene conjugates on the 180th and 210th days of development by 63.6% and 44.1%, respectively.This is confirmed by a significant increase in the number of secondary products of peroxidation - TBA-active complexes in these periods of their development. Studyrevealed that during puberty of gilts the antioxidant defense system wasthe most labile from 6 to 8 months of age, where the variability of PAG components wassignificant in terms of increased activity of superoxide dismutase (p &lt;0.01) and catalase (p &lt;0.05), as well as the amount of reduced glutathione. Moreover, additional feeding of HDLcontributedto the probable slowing down of peroxidation processes in blood, which was confirmed by the lower content of diene conjugates (p &lt;0.05-0.01) and TBA-active complexes (p &lt;0.01-0.001).<br /><br /><strong>Conclusions:</strong> Study concluded that experimental supplementary feeding ofHDLdiet to LWgiltsstimulatedthe reproductive function during their puberty by reducing the time to the first estrous cycle for artificial insemination, thus improved the fertility in LW gilts. 2021-01-24T00:00:00+01:00 Copyright (c) 2021 Translational Research in Veterinary Science https://apcz.umk.pl/TRVS/article/view/TRVS.2020.009 Benchmark of algorithms for multiple DNA sequence alignment across livestock species 2021-01-25T10:50:09+01:00 Artur Bąk artur.bak@izoo.krakow.pl Grzegorz Migdałek grzegorz.migdalek@up.krakow.pl Chandra Shekhar Pareek pareekcs@umk.pl Kacper Żukowski kzukowski@umk.pl <strong>Background:</strong> Due to the growing amount of biological data, it is often necessary to select the most optimal estimation method for DNA sequence alignment across livestock species. One of the most important benches of genomics is to modelling homology between considered DNA sequences. A multiple sequence alignment is a potent tool for molecular and evolutionary biology, and there are several programs and algorithms applicable for this purpose. The purpose of this paper was to study the most commonly used DNA alignment algorithms to select the optimal tool dedicated for short sequences.<br /><br /><strong>Methods:</strong> Four steps of bioinformatics pipelines were considered to benchmark the algorithms for multiple DNA sequence alignment across livestock species: 1) selection of reference genome sequences of ARS1.2 for cattle, EquCab3.0 for horse and vicPac2 for alpaca with a low E-value using TBLASTn 2) removing gaps for these sequences 3) alignment of obtained sequences using examined algorithms 4) matching the quality of aligned sequences with sequences of reference genomes by more software. The time of computation was archived for the whole analysis. The seven programs were utilized, each based on different alignment algorithms, namely: ClustalO, ClustalW, Kalign, MAFFT, MUSCLE, Probcons and T-Coffee.<br /><br /><strong>Results:</strong> The result obtained in this study showed that the fastest is progressive algorithms such as Kalign or MUSCLE-FAST. Moreover, the iterative algorithms like MAFFT and MUSCLE revealed a higher quality of the alignment. The T-Coffee and Probcons programs were computational cost-effective; simultaneously, they were generating a medium-quality calculation in a relatively long time. The best quality of alignment was shown by iterative variants of the MAFFT program; however, the speed of the calculations was relatively low. The fastest algorithm was Kalign, making alignment much faster than the competitors, but achieving average results in the quality of the alignment. The average speed ratio concerning the quality of the analyzed algorithms was obtained by the progressive version of MAFFT, NS1.<br /><br /><strong>Conclusions:</strong> We conclude that the results of this study can be used to re-alignment of variant primers in new livestock genome releases. 2021-01-24T00:00:00+01:00 Copyright (c) 2021 Translational Research in Veterinary Science