Analysis of ultrastructural morphometric changes of pigeon kidneys affected by colibacteriosis
Keywordsmorphometry, patho-morphology, renal, kidney, nephrocyte, avian, pigeons, colibacteriosis, histo-morphology
Abstract. The article provides data on the morphometric changes in the kidneys of pigeons with colibacteriosis. Patho-morphological examination of the kidneys revealed focal hyperemia of the kidney cortical area and edema of the loose fibrous tissue. The convoluted renal tubule epitheliocytes were enlarged in size. In this case, the tubules get narrow and become slit-shaped. The convoluted tubules epithelium showed signs of granular dystrophy. In the chronic course of colibacteriosis, granuloma formation and signs of chronic interstitial nephritis were observed, in which the loose connective tissue of the cortical area is replaced by fibrous tissue. Morphometric analysis revealed that in the experimental group there was a decrease in the nephrocyte diameter (p= <0.05) to 98.06±0.46µm and the nephrocyte area to 6319.38±9.62µm. This results in a significant reduction (p= <0.05) of the sub-capsular space to 11.14±0.16µm compared to the control group. The distal and proximal convoluted tubules diameter also decreased to 127.43±20.78μm and 39.50±1.63μm, respectively. Accordingly, the area of the distal and proximal convoluted tubules decreased significantly (p= <0.05) to 10164.10±0.43µm and 2974.43±4.01µm, respectively. The patho-genetic effect of E. coli toxins revealed the renal tubular obstruction and slowing of the reabsorption and urinary excretion processes resulting in a reduction of glomerular filtration capacity of the kidney. Due to the prolonged course of salmonellosis occurring during the latent course, the area of renal filtration was also reduced. Accumulation of nitrogen metabolism products that enter the bloodstream caused impaired water-salt metabolism and acid-base equilibrium.
Nedosekov V. Infectious animal pathology: problems and prospects. Earth Bioresources and Life Quality. 2012;1. http://gchera-ejournal.nubip.edu.ua/index.php/ebql/article/view/14
Nedosekov V., Gomzykov O. Biological properties of salmonella isolated from chickens in Ukraine farms. Earth Bioresources and Life Quality. 2013;3. http://gchera-ejournal.nubip.edu.ua/index.php/ebql/article/view/108/pdf.
Konicek C, Vodrážka P, Barták P, Knotek Z, Hess C, Račka K, Hess M, Troxler S. Detection of zoonotic pathogens in wild birds in the cross-border region Austria, Czech Republic. J Wild Dis. 2016;52: 850-861.
Toro, H., Saucedo, C., Borie, C., Gough, R. E. and Alcaino, H. Health status of free-living pigeons in the city of Santiago. Avian Pathol. 1999;28: 619-623.
Muzyka D, Pantin-Jackwood M, Stegniy B, Rula O, Bolotin V, Stegniy A, Gerilovych A, Shutchenko P, Stegniy M, Koshelev V, Maiorova K, Tkachenko S, Muzyka N, Usova L, Afonso CL. Wild bird surveillance for avian paramyxoviruses in the Azov-black sea region of Ukraine (2006 to 2011) reveals epidemiological connections with Europe and Africa. Appl Environ Microbiol. 2014;80: 5427-5438.
Gargiulo A, Fioretti A, Russo TP, Varriale L, Rampa L, Paone S, De Luca Bossa LM, Raia P, Dipineto L. Occurrence of enteropathogenic bacteria in birds of prey in Italy. Lett Appl Microbiol. 2018;66:202-206.
Belozerov E, Kiseleva L, Midlenko V, Kuzmina A, Romanenko S, Reznikova E. Infectious diseases and biological safety problems. Ulyanovsk Medical Biological Journal. 2016;3: 8-15.
Carver S, Kilpatrick M, Kuenzi A, Douglass R, Ostfeldd R, Weinsteine P. Environmental monitoring to enhance comprehension and control of infectious diseases. The Royal Society of Chemistry. 2010;12: 2048-2055.
Kumar A, Bhalerao D, Gupta RP, Kumari M. Pathological studies on natural cases of avian colibacillosis in Haryana state. Haryana Vet. 2013;52: 118-120.
Rahman MA., Samad MA., Rahman MB., Kabir SML. Bacterio-pathological Studies on Salmonellosis, Colibacillosis and Pasteurellosis in Natural and Experimental Infections in Chickens. Avian Medicine. 2004;1:1-8.
Wernicki A, Nowaczek A, Urban-Chmiel R. Bacteriophage therapy to combat bacterial infections in poultry. Virol J. 2017; 14: 179.
Kupczyński R, Szumny A, Bednarski M, Piasecki T, Śpitalniak-Bajerska K, Roman A. Application of Pontentilla Anserine, Polygonum aviculare and Rumex Crispus Mixture Extracts in A Rabbit Model with Experimentally Induced E. coli Infection. Animals (Basel) 2019; 9: 774.
Zakari OF, Ayo JO, Rekwot PI, Kawu MU. Effect of age, sex, physical activity and meteorological factors on haematological parameters of donkeys (Equus asinus). Comp Clin Path. 2016; 25: 1265–1272.
Sharma V, Jakhar KK, Nehra V, Kumar S. Biochemical studies in experimentally Escherichia coli infected broiler chicken supplemented with neem (Azadirachta indica) leaf extract. Vet World. 2015; 8: 1340–1345.
Kolenda R, Burdukiewicz M, Schierack P. A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front Cell Infect Microbiol. 2015; 5: 23.
Abalaka SE, Sani NA, Idoko IS, Tenuche OZ, Oyelowo FO, Ejeh SA, Enem SI. Pathological changes associated with an outbreak of colibacillosis in a commercial broiler flock. Sokoto Journal of Veterinary Sciences. 2017;15: 95-102.
Mitsyk AV. Using ImageJ for automatic morphometry in histological examinations. Omsk Scientific Journal of Information Technologies. 2011;100: 187-189.
Malish NG, Chemich MD, Kovalenko OI. Modern features of the epidemic process of salmonellosis. Infectious diseases. 2013;4: 30-35.
Shestakov VA, Lysko SB. Histostructural changes in the liver and kidneys are induced by esherihiosis. Veterinary Journal. 2015;73: 19-22.
How to Cite
Title, logo and layout of TR in VS are reserved trademarks of TR in VR.
Number of views and downloads: 189
Number of citations: 0