INFLUENCE OF HUMILID DRUG ON THE QUALITY OF SPERM PRODUCTION IN BREEDING BOARS UNDER DIFFERENT REGIMES OF USE
DOI:
https://doi.org/10.12775/TRVS.2021.003Keywords
breeding boars, regimes of use, semen, sperm production, humin compoundsAbstract
Background: Studies have shown the influence of the Red and White Belted meat breeding boars’ usage regimes on the quality of sperm products under the effect of “Humilid” biologically active feed supplement. The data obtained in the control group of breeding boars are explained by their intensive use and significant consumption of body resources to maintain sexual activity. Due to the adaptogenic properties of the drug, which had a positive effect on the quality of sperm production in the experimental group of animals on the 30th day of the experiment, the body’s resources were mobilized. It is established that consumption of biologically and ecologically safe feed supplements of humin nature permits to obtain high-quality and complete sperm products under different regimes of using breeding boars.
Methods: For the experiment, 10 animals of similar age and live weight were selected, 5 of which were the control group that did not receive the 'Humilid’ drug and 5 animals that received a biologically active feed supplement with food. During the experiment, different regimes of boar usage were applied (once, twice and three times a week) for 60 days. The experiment was divided into three stages (preparatory, main, and final), during which sperm quality indices were monitored: ejaculate volume, sperm concentration, total number, and number of live sperm, their motility, and survival during incubation.
Results: In the previous period, the following dynamics in the effect of the effect of the regimens on sperm quality was observed: the twofold regime showed a decrease in ejaculate volume in the control group by 18.7 % and in the experimental group by 7.2 % compared to a single semen collection regimen. In the main period, the decrease in ejaculate volume was 17.6 % (p<0.001) and 5.4 % (p<0.001), respectively, and at the final stage – by 15.8 % (p<0.001) and 9.1 % (p<0.001). At that time, sperm motility and survival rates were the highest in animals that received “Humilid” biologically active feed supplement and had a sexual load twice a week. Sperm motility in the preparatory period in the control group was 90.4 % and in the experimental group – 95.0 %, survival – 84.0 % and 90.8 %, respectively. During the main phase of the experiment, sperm motility was within the range of 85.9 % and 98.8 % and survival – 78.5 % and 95.3 %, respectively. In contrast, in the final stage, sperm motility was 89.6 % and 92.0 % and survival was 82.0 % and 88.9 %, respectively.
References
[1] Oberlender, G., Murgas, L. D. S., Zangeronimo, M. G., da Silva, A. C., Pereira, L. J. Influence of ejaculation time on sperm quality parameters in high performance boars. Journal of Animal Science Advance., 2012; 2 (5): 499-509.
[2] Schulze, M., Buder, S., Rüdiger, K., Beyerbach, M., Waberski, D. Influences on semen traits used for selection of young AI boars. Animal Reproduction Science., 2014; 148 (3-4): 164-170. DOI: 10.1016/j.anireprosci.2014.06.008
[3] Robinson, J. A. B., Buhr, M. M. Impact of genetic selection on management of boar replacement. Theriogenology., 2005; 63 (2): 668–678. DOI: 10.1016/j.theriogenology.2004.09.040
[4] Okere C., Joseph A., Ezekwe M. Seasonal and genotype variations in libido, semen production and quality in artificial insemination boars. Journal of Animal and Veterinary Advances., 2005; 4, 10: 885-888.
[5] Wolf J., Smital J. Quantification of factors affecting semen traits in artificial insemination boars from animal model analyses. Journal of Animal Science., 2009b; 87: 1620-1627.
[6] Oh, S. H., See, M. T., Long, T. E., Galvin, J. M. Estimates of genetic correlations between production and semen traits in boar. Asian–Australasian Journal of Animal Sciences., 2006; 19 (2): 160–164. DOI: http://dx.doi.org/10.5713/ajas.2006.160
[7] Rutten, S. C., Morrison, R. B., Reicks, D., Boar stud production analysis. Journal of Swine Health and Production., 2000; 8 (1): 11-14.
[8] Smital, J. Effects influencing boar semen. Animal Reproduction Science, 2009; 110 (3-4): 335-346. DOI: 10.1016/j.anireprosci.2008.01.024
[9] Clark, S. G., Schaeffer, D. J., Althouse, G. C. B-Mode ultrasonographic evaluation of paired testicular diameter of mature boars in relation to average total sperm numbers. Theriogenology., 2003; 60 (6): 1011–1023. DOI: 10.1016/S0093-691X(03)00127-4.
[10] Colenbrander, B., Feitsma, H., Grooten, H. J. Optimizing semen production for artificial insemination in swine. Journal of Reproduction and Fertility, Supplement., 1993; 48: 207-215.
[11] Colenbrander, B., Kemp, B. Factors influencing semen quality in pigs. Journal of Reproduction and Fertility, Supplement., 1990; 40: 105–115.
[12] Kondracki S., Wysokińska A., Kowalewski D., Muczyńska E., Adamiak A. Season’s influence on the properties of male domestic pig semen. Rozprawy naukowePope John Paul II State School of Higher Vocational Education in Biała Podlaska, 2009; Vol. III; 177-187.
[13] Kunowska-Slósarz M., Makowska A. Effect of breed and season on the boar’s semen characteristics. Annals of Warsaw University of life sciences – SGGW, Animal Science, 2011; 49: 77-86.
[14] Ciereszko, A., Ottobre, J. S., Glogowski, J. Effects of season and breed on sperm acrosin activity and semen quality of boars. Animal Reproduction Science., 2000; 64: (1-2), 89–96. DOI: 10.1016/S0378-4320(00)00194-9.
[15] Wysokińska, A., Kondracki, S., Kowalewski, D., Adamiak, A., Muczyńska, E. Effect of seasonal factors on the ejaculate properties of crossbred Duroc x Pietrain and Pietrain x Duroc boars as well as purebred Duroc and Pietrain boars. Bulletin of the Veterinary Institute in Pulawy., 2009; 53: (4), 677-685. 271
[16] Rivera, M. M., Quintero-Moreno, A., Barrera, X., Palomo, M. J., Rigau, T., Rodriguez-Gil, J. E. Natural Mediterranean photoperiod does not affect the main parameters of boar-semen quality analysis. Theriogenology., 2005; 64: (4), 934-946. DOI: 10.1016/j.theriogenology.2005.01.001
[17] Kemp, B., den Hartog, L. A. The influence of energy and protein intake on the reproductive performance of the breeding boar: a review. Animal Reproduction of Science., 1989; 20: (2), 103–115. DOI: 10.1016/0378-4320(89)90068-7
[18] Frangež R., Gider T., Kosec M. Frequency of boar ejaculate collection and its influence on semen quality, pregnancy rate and litter size. Acta Vet. Brno., 2005; 74, 265-273.
[19] Bonet, S., Briz, M., Fradera, A. The sperm quality and fertility of boars after two different ejaculation frequencies. Scientia Gerundensis., 1991; 17, 77- 84.
[20] Julianni Dornelles, Carlos Augusto Rigon Rossi, Luara Medianeira de Lima Schlösser, Rodrigo Dalmina Rech, Cristian Guilherme Gräf, Cínthia Melazzo de Andrade, Marcelo Soares, Marcos Speroni Ceron & Jovani Patias. Intervalos de coletas de sêmen em reprodutores suínos no início da vida reprodutiva: avaliação dos parâmetros seminais. Acta Scientiae Veterinariae., 2019; 47, 1714.
[21] Umesiobi, D. O. Boar effects and their relations to fertility and liter size in sows. South African Journal of Animal Science., 2010; 40 (5, Supplement 1), 471-475. ISSN 2221-4062
[22] Kondracki, S., Wysokińska, A., Iwanina, M., Banaszewska, D., Sitarz, D. Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars. Polish Journal of Veterinary Sciences., 2011; 14: (1), 35-40. DOI: 10.2478/v10181-011-0005-z
[23] Kondracki, S., Banaszewska, D., Bajena, M., Komorowska, K., Kowalewski, D. Correlation of frequency of spermatozoa morphological alterations with sperm concentration in ejaculates of Polish Landrace boars. Acta Veterinaria., 2013a; 63: (5-6), 513-524. DOI: 10.2298/AVB1306513K
[24] Rijsselaere, T., Van Soom, A., Hoflack, G., Maes, D., Kruif, A. Automated sperm morphometry and morphology analysis of canine semen by the Hamilton-Thorne analyser. Theriogenology., 2004; 62, 1292-1306. DOI: 10.1016/j.theriogenology.2004.01.005
[25] de Paz, P., Mata-Campuzano, M., Tizado, E. J., Álvarez, M., Álvarez-Rodríguez, M., Herraez, P., Anel, L. The relationship between ram sperm head morphometry and fertility depends on the procedures of acquisition and analysis used. Theriogenology., 2011; 76: (7), 1313-1325. DOI: 10.1016/j.theriogenology.2011.05.038
[26] Hirai, M., Boersma, A., Hoeflich, A., Wolf, E., Föll, J., Aumüller, T. R., Braun, J. Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): relation to fertility and seminal plasma growth factors. Journal of Andrology., 2001; 22: (1), 104-110.
[27] Ostermeier, G. C., Sargeant, G. A., Yandell, B. S., Evenson, D. P., Parrish, J. J. Relationship of bull fertility to sperm nuclear shape. Journal of Andrology., 2001; 22: (4), 595-603.
[28] Kondracki, S., Iwanina, M., Kowalewski, D., Bajena, M., Wysokińska, A., Dependence of physical characteristics of ejaculate of Polish Landrace boars on the time interval between successive ejaculate collections. Scientific Annals of Polish Society of Animal Production., 2018; Vol. 14: (2), 21-34.
[29] Kommisrud, E., Paulenz, H., Sehested, E., Grevle, I. S. Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta Veterinaria Scandinavica., 2002; 43, 49-55. DOI: 10.1186/1751-0147-43-49
[30] Strzeżek, J., Korda, W., Glogowski, J., Wysocki, P., Borkowski, K. Influence of semen-collection frequency on sperm quality in boars, with special reference to biochemical markers. Reproduction in Domestic Animals., 1995; 30: 2, 85-94. DOI: 10.1111/j.1439-0531.1995.tb00609.x 270
[31] Kondracki, S., Górski, K., Wysokińska, A., Jóźwik, I. Correlation of ejaculate parameters and sperm morphology with the ejaculate volume of Pietrain boars. Bulgarian Journal of Agricultural Science., 2014; 20: 3, 721-727.
[32] Miclea, V., Záhan, M., Miclea, I., Vajda, I. Influence of harvest frequency on the quality of boar semen. Bulletin University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal science and biotechnologies., 2007; 64: (1-2).
[33] MacCarthy P. The principles of humic substances. Soil Sci., 2001; 166, 738–751.
[34] Lenk V.T., Benda A. Torfpaste-ein huminsa¨urehaltiges tiergesundheitspflegemittel zur vorbeuge und behandlung von durchfallerkrankungen der ka¨lber. Monatshefte Vet., 1989; 44, 563–565.
[35] Griban V.G., Baranahenko V.A., Kasyan S.S., Verlos S.V. Use of Hydrohumate (sodium salt of humic acid) for enhancing the natural resistance of cows with subclinical nutritional disorders. Vet. Moskova., 1991; 12, 54–56.
[36] Ku¨hnert V.M., Bartels K.P., Kro¨ll S., Lange N. Huminsa¨urehaltige tierarzneimittel in therapie and prophylaxes bei gastrointestinalen erkrankungen von hund undkatze. Monatshefte Vet., 1991; 46, 4–8.
[37] EMEA. Humic acids and their sodium salts, summary report. Committee for veterinary medicinal products. Eur. Agency Eval. Med. Prod., 1999. http://www.emea.eu.int/pdfs/vet/mrls/055499en.
[38] Klocking R., Helbig B., Schotz G., Schacke M., Wutzler P. Anti-HSV-1 activity of synthetic humic acid-like polymers derived from p-diphenolic starting compounds. Antivi. Chem. Chemother., 2002; 13, 241–249.
[39] Van Rensburg C.E., Dekker J., Weis R., Smith T.L., Van Rensburg E.J., Schneider J. Investigation of the anti- HIV properties of oxihumate. Chemotherapy., 2002; 48:138–143.
[40] Shvetsova, O. M., Vplyv biolohichno aktyvnoi kormovoi dobavky Humilid na fiziolohichnyi status ta produktyvni yakosti. Naukovo-tekhnichnyi biuleten naukovo-doslidnoho tsentru biobezpeky ta ekolohichnoho kontroliu resursiv APK., 2014; 2: 1. http://biosafety-center.com/naukovi_vydannya/pdf/2_12.pdf
[41] Bassols, J., Bussalleu, E., Yeste, M., Bonet, S. Effects of a high semen-collection frequency on the quality of sperm from ejaculates and from six epididymal regions in boars. Theriogenology., 2005; 63: 8, 2219-2232. DOI: 10.1016/j.theriogenology.2004.10.009
[42] Ji, F., McGlone, J.J. & Kim, S.W., Effects of dietary humic substances on pig growth performance, carcass characteristics and ammonia emission. Anim. Sci.. 2006; 84, 2482—2490. DOI:10.2527/jas.2005-206.
[43] Xu, Z.R., Feng, J., Studies on effects of Betain on carcass characteristics in finishing swine. Acta Verterinria et Zootechnica Sinca (in chinese)., 1998; 29, 397 – 405.
[44] Wang, Q., Chen, Y.J., Yoo, J.S., Kim, H.J., Cho, J.H., Kim, I.H., Effects of supplemental humic substances on growth performance, blood characteristics and meat quality in finishing pigs. Livestock Sci,. 2008; Vol. 117: 2—3, 270—274. DOI: http:// dx.doi.org/10.1016/j.livsci.2007.12.024/
[45] Shostia, A. M., Rokotianska, V.O., Dynamika yakosti spermoproduktsii u knuriv-plidnykiv zalezhno vid pory roku ta intensyvnosti yikh vykorystannia. Svynarstvo., 2018; 71, 116-123. http://dspace.pdaa.edu.ua:8080/handle/123456789/5321
[46] Bajena, M., Kondracki, S., Iwanina, M., Wysokińska, A., Adamiak, A., Physical characteristics of ejaculates produced by insemination boars depending on the interval between successive ejaculate collections. Journal of Central European Agriculture., 2016; 17: 2, 260-271.
Corresponding author: Inga Pavlova, Poltava State Agrarian Academy, 1/3 Skovorody str., Poltava, Ukraine, inga17pavlova@gmail.com Phones: +38 (066)4288586,
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