The Activity of Lysosomal Enzymes in The Healthy Men’S Blood After Single Finnish Sauna Procedure – Preliminary Study
DOI:
https://doi.org/10.12775/v10251-012-0052-4Keywords
Finnish sauna, acid phosphatase, cathepsin D, arylsulfatase, α-1-antitrypsinAbstract
Introduction . The aim of the paper was to investigate the effect of single Finnish sauna procedure on the activity of acid phosphatase (AcP), cathepsin D (CTS D), arylsulfatase (ASA) and inhibitor of proteases α-1-antitrypsin (AAT) in healthy men’s blood.
Materialandmethods. Men (n=7) performed sauna procedure (3 entries, each 10 min) in temperature of 90oC and relative air humidity of 10%. Men cooled their whole body (cold shower) after each exit from sauna. The blood was taken from basilic vein before the entry to sauna, 15 and 60 min after the procedure. Obtained results of activity of AcP, AAT and ASA were statistically analyzed by using ANOVA test. Statistically analysis of CTS D activity was performed by Mann-Whitney test. The changes of the level p<0.05 were accepted as statistically significant.
Results. Statistically significant (p<0.01) decrease of the CTS D activity of about 51.4% 60 min after sauna was revealed as compared to the activity of this enzyme before entry to sauna (control). Furthermore, the tendency to decrease of the activity of CTS D and AcP 60 min after sauna was found in comparison to the activity of these hydrolases measured 15 min after the procedure (p>0.05). The tendency was also observed when comparing the activity of AcP, ASA, CTS D and AAT 15 min after sauna to their activity before sauna (p>0.05). In turn, the activity of AAT and ASA insignificantly increased 60 min after Finnish sauna comparing to the activity measured 15 min after procedure (p>0.05).
Conclusion. Single Finnish sauna procedure increases stabilization of lysosomal membranes.
References
Prystupa T et al. The effects of Finish sauna on hemodynamics of the circulatory system in men and women. J Hum Kinet 2009; 22:61-68.
Saunas and your health [tekst redakcyjny]. Harvard Men’s Health Watch. 2005; 6-7.
Biro S et al. Clinical Implications of Thermal Therapy in Lifestyle-Related Diseases. Exp Biol Med 2003; 228:1245-1249.
Hannuksela ML, Ellahham S. Benefits and risks of sauna bathing. Am J Med. 2001; 110(2):118-26. [ http://dx.doi.org/10.1016/S0002-9343(00)00671-9]
Kukkonen-Harjula K, Kauppinen K. Health effects and risks of sauna bathing. Int J Circumpolar Heath 2006; 65(3):195-205.
Pilch W et al. Changes in the lipid profile of blood serum in women taking sauna baths of various duration. Int J Occup Med Environ Health. 2010; 23(2):167-174.
Scoon GSM et al. Effect of post-exercise sauna bathing on the endurance performance of competitive male runners. J Sci Med Sport 2007; 10:259-262. [ http://dx.doi.org/10.1016/j.jsams.2006.06.009]
Imai Y et al. Acute myocardial infarction induced by alternating exposure to heat in a sauna and rapid cooling in cold water. Cardiology 1998; 90:299-301. [ http://dx.doi.org/10.1159/000006862]
Rajewski P, Rajewski P. Lizosomy i enzymy lizosomalne - budowa i funkcje, ze szczególnym uwzględnieniem katepsyny D jako nowego markera chorób nowotworowych. Family Med Prim Care Rev 2007; 9(2):275-278.
Bartosz G. Druga twarz tlenu. PWN. Warszawa 2009.
Mila-Kierzenkowska C et al. The activity of lysosomal enzymes in blood serum of elite kayakers after the whole-body cryostimulation and exercise. Sport vs Welness. 2008; VI: 51-60.
Woźniak A i wsp. Aktywność wybranych enzymów lizosomalnych w surowicy krwi kajakarzy po kriostymulacji ogólnoustrojowej. Med Sport 2001; 17 (8): 322-327.
Colowick SP, Kaplan NC. Methods in enzymology. Vol. 2 Academic Press. New York 1955.
Krawczyński J. Diagnostyka enzymologiczna w medycynie praktycznej. Metodyka badań. PZWL. Warszawa 1972.
Błeszyński W, Działoszyński LM. Purification of soluble arylsulfatase from ox brain. Biochem J 1965; 97:360-364.
Szczeklik E. Enzymologia kliniczna. PZWL. Warszawa 1974.
Szmidt M i wsp. Zachowanie się alfa-1-antytrypsyny oraz jej fenotypy u chorych na astmę z nadwrażliwością na aspirynę. Pneumonol Alergol Pol 1991; 59:114-120.
Sutkowy et al. The effect of Finnish sauna on the activity of selected lysosomal enzymes on healthy subjects. Ovidius University Annals. Series Physical Education and Sport/Science. Movement and health 2011b; 11(2): 286-291.
Sutkowy i wsp. Stężenie substancji reagujących z kwasem tiobarbiturowym (TBARS) oraz witamin A i E we krwi osób amatorsko uprawiających sport po jednorazowej kriostymulacji ogólnoustrojowej. Med Sport 2011a; 27:43-54.
Pall ML. Do sauna therapy and exercise act by raising the availability of tetrahydrobiopterin? Med Hypotheses 2009; 73:610-613.
Babu et al. Lysosomal membrane stabilization and antiinflammatory activity of Clerodendrum phlomidis L.f., a traditional medicinal plant. J Ethnopharmacol 2011; 135(3):779-85.
Woźniak A i wsp. Peroksydacja lipidów we krwi kajakarzy po kriostymulacji ogólnoustrojowej i treningu fizycznym. Med Sport 2007; 23:15-22.
Tsuboi et al. Intralysosomal pH and release of lysosomal enzymes in the rat liver after exhaustive exercise. J Appl Physiol. 1993; 74:1628-1634.
Downloads
Published
How to Cite
Issue
Section
Stats
Number of views and downloads: 458
Number of citations: 0
