Enterobacteriaceae strains with reduced susceptibility to carbapenems

Abstract

The aim of the study was to estimate the prevalence of Enterobacteriaceae with reduced susceptibility to carbapenems. Forty-five strains of Enterobacteriaceae with reduced susceptibility to carbapenems were investigated. The strains were cultured from patients treated at the Dr Antoni Jurasz University Hospital no.1 in Bydgoszcz. Identification of the strains was carried out based on the results of biochemical reactions performed in identification cards GN (bioMérieux). Susceptibility testing to carbapenems and determining Minimal Inhibitory Concentration (MIC) was carried out using E - test (bioMérieux) (for ertapenem and imipenem), and agar dilution method for meropenem. The analysed strains were isolated from: urine – 13 strains (28.9%), blood and bronchoalveolar lavage - six strains from each (13.3% ), 5 – wound swabs (11.2%) and other clinical materials – 15 (33.3%). 53.3% of the strains were identified as Klebsiella pneumoniae. 93.4% of the isolates were susceptible to meropenem. Only 4.5% of the analysed strains were susceptible to ertapenem. None of the analyzed strains produced carbapenemases.